Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,575 T→C 23.4% V341V (GTA→GTG arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,5750TC23.4% 162.9 / 50.3 94V341V (GTA→GTGarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base T (40/32);  new base C (8/14);  total (48/46)
Fisher's exact test for biased strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01

GGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGG  >  Exported/2595506‑2595642
                                                                     |                                                                   
ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt                                                                     <  1:4262823/70‑1 (MQ=255)
ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt                                                                     <  1:1822723/70‑1 (MQ=255)
ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt                                                                     <  1:1472548/70‑1 (MQ=255)
 gCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa                                                                    <  1:2396974/70‑1 (MQ=255)
 gCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa                                                                    <  1:3422995/70‑1 (MQ=255)
    tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt                                                                >  1:1124572/1‑71 (MQ=255)
     aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc                                                                   >  1:1088319/1‑67 (MQ=255)
     aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc                                                                   >  1:1249439/1‑67 (MQ=255)
     aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                               >  1:2560031/1‑71 (MQ=255)
      aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                               <  1:4005571/70‑1 (MQ=255)
      aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                              >  1:4762948/1‑71 (MQ=255)
      aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                              >  1:1911825/1‑71 (MQ=255)
      aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                              >  1:190643/1‑71 (MQ=255)
      aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                              >  1:4005558/1‑71 (MQ=255)
      aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                              >  1:4571849/1‑71 (MQ=255)
             gcctcttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCatat                                                        >  1:300896/4‑70 (MQ=255)
             gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                                                       >  1:285528/1‑71 (MQ=255)
             gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                                                       <  1:4176290/71‑1 (MQ=255)
              cctcttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTg                                                      <  1:4234961/69‑1 (MQ=255)
                 cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                                                     <  1:1743118/69‑1 (MQ=255)
                  tttGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGtt                                                    <  1:1311838/69‑1 (MQ=255)
                    tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt                                                                >  1:389031/1‑55 (MQ=255)
                    tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat                                                 <  1:278853/70‑1 (MQ=255)
                     gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                                               >  1:467426/1‑71 (MQ=255)
                     gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                                               >  1:900912/1‑71 (MQ=255)
                      aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                                                     >  1:5610185/1‑64 (MQ=255)
                       cgaCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTc                                                   <  1:2852098/63‑1 (MQ=255)
                         aCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat                                                 <  1:5301797/65‑1 (MQ=255)
                         aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATaga                                           <  1:1448564/71‑1 (MQ=255)
                         aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATaga                                           <  1:1441744/71‑1 (MQ=255)
                         aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                            <  1:4980414/70‑1 (MQ=255)
                         aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                            <  1:364533/70‑1 (MQ=255)
                         aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                            <  1:1061604/70‑1 (MQ=255)
                         aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                            <  1:5685742/70‑1 (MQ=255)
                          cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa                                          <  1:464555/71‑1 (MQ=255)
                          cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa                                          <  1:3890566/71‑1 (MQ=255)
                           gCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCatca                                               >  1:1893540/1‑63 (MQ=255)
                           gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaag                                         <  1:1003764/71‑1 (MQ=255)
                            ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc                                                                  <  1:2483012/45‑1 (MQ=255)
                            ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                        <  1:1625058/71‑1 (MQ=255)
                               gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag                                     <  1:5283863/71‑1 (MQ=255)
                                  gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                               >  1:1142167/1‑42 (MQ=255)
                                  gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                               >  1:1026562/1‑42 (MQ=255)
                                  gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                               >  1:5313032/1‑42 (MQ=255)
                                  gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                               >  1:5695721/1‑42 (MQ=255)
                                  gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                               >  1:3029323/1‑42 (MQ=255)
                                  gCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTg                                                      >  1:3789575/1‑51 (MQ=255)
                                  gCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGt                                                     >  1:2675742/1‑52 (MQ=255)
                                   ccGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGtt                                                    >  1:4158798/1‑52 (MQ=255)
                                   ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat                                                 <  1:4648604/55‑1 (MQ=255)
                                   ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat                                                 <  1:5532385/55‑1 (MQ=255)
                                   ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat                                                 <  1:1134433/55‑1 (MQ=255)
                                    cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg                                >  1:4332174/1‑69 (MQ=255)
                                      tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCgg                                                             <  1:3779870/40‑1 (MQ=255)
                                      tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCgg                                                             <  1:2187126/40‑1 (MQ=255)
                                      tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCg                              <  1:2098620/71‑1 (MQ=255)
                                        ctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCat                                                          >  1:569487/1‑41 (MQ=255)
                                           acATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAAAAGAAGAGAGGAAGGCGGTGAg                         >  1:2566778/2‑71 (MQ=255)
                                            cgtCAATCGACAGCGCACCAATCAGTACCGTCGGCATATTGTTCat                                                 >  1:1992271/3‑46 (MQ=25)
                                            cgtCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat                                                 >  1:566624/3‑46 (MQ=255)
                                            cATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                                               >  1:3032727/1‑48 (MQ=255)
                                              tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                                                      <  1:1539955/39‑1 (MQ=255)
                                              tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                                                      <  1:3412685/39‑1 (MQ=255)
                                              tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                                                      <  1:2668579/39‑1 (MQ=255)
                                              tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa                      <  1:4671686/71‑1 (MQ=255)
                                               cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt                     >  1:606189/1‑71 (MQ=255)
                                                aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                                >  1:2973408/1‑43 (MQ=255)
                                                aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                                >  1:2090024/1‑43 (MQ=255)
                                                aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                                >  1:1108406/1‑43 (MQ=255)
                                                aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                                >  1:1973001/1‑43 (MQ=255)
                                                aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagag                                       >  1:2789313/1‑52 (MQ=255)
                                                aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc                    <  1:3490046/71‑3 (MQ=255)
                                                aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc                    <  1:4929070/71‑3 (MQ=255)
                                                 aTGGACAACCCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc                    >  1:3660895/1‑70 (MQ=255)
                                                  tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                            <  1:2407353/45‑1 (MQ=255)
                                                  tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                            <  1:3300775/45‑1 (MQ=255)
                                                  tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                            <  1:4363712/45‑1 (MQ=255)
                                                  tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                            <  1:3637119/45‑1 (MQ=255)
                                                  tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt                            <  1:218872/61‑1 (MQ=255)
                                                    gACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt                <  1:3523388/71‑1 (MQ=255)
                                                    gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa                                  >  1:1057806/1‑53 (MQ=255)
                                                      cAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGc              >  1:3578976/1‑71 (MQ=255)
                                                        gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg            <  1:3499369/70‑1 (MQ=255)
                                                        gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg            <  1:4148179/70‑1 (MQ=255)
                                                         cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                        >  1:2020014/1‑42 (MQ=255)
                                                         cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                        >  1:800238/1‑42 (MQ=255)
                                                         cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                        >  1:5598692/1‑42 (MQ=255)
                                                         cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                 >  1:1755250/1‑65 (MQ=255)
                                                            accAATCAGCACCGTCGGCATATTGTTCATGACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGaac         <  1:4954810/69‑3 (MQ=255)
                                                            accAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACg        >  1:5332894/2‑71 (MQ=255)
                                                             ccAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt       >  1:1209201/1‑71 (MQ=255)
                                                              cAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTc      <  1:5586629/71‑1 (MQ=255)
                                                              cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt       <  1:4575829/70‑1 (MQ=255)
                                                              cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTc      >  1:5083682/1‑71 (MQ=255)
                                                               aatcAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTcg     >  1:1122560/4‑71 (MQ=255)
                                                               aaCCAGTACCGTCGGCATATTGTTCATAATagaaga                                        <  1:579353/36‑1 (MQ=255)
                                                                  cAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCgg  >  1:4912015/1‑71 (MQ=255)
                                                                     |                                                                   
GGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGG  >  Exported/2595506‑2595642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: