Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,596 A→C 25.0% I334M (ATT→ATG) ‡ arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,5960AC25.0% 74.1 / 37.9 59I334M (ATT→ATG) ‡arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (19/25);  new base C (5/9);  total (24/35)
Fisher's exact test for biased strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01

GATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCA  >  Exported/2595527‑2595666
                                                                     |                                                                      
gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                                                                       >  1:900912/1‑71 (MQ=255)
gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                                                                       >  1:467426/1‑71 (MQ=255)
    aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATaga                                                                   <  1:1448564/71‑1 (MQ=255)
    aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATaga                                                                   <  1:1441744/71‑1 (MQ=255)
    aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                                                    <  1:4980414/70‑1 (MQ=255)
    aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                                                    <  1:5685742/70‑1 (MQ=255)
    aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                                                    <  1:364533/70‑1 (MQ=255)
    aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                                                    <  1:1061604/70‑1 (MQ=255)
     cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa                                                                  <  1:3890566/71‑1 (MQ=255)
     cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa                                                                  <  1:464555/71‑1 (MQ=255)
      gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaag                                                                 <  1:1003764/71‑1 (MQ=255)
       ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                                                <  1:1625058/71‑1 (MQ=255)
          gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag                                                             <  1:5283863/71‑1 (MQ=255)
               cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg                                                        >  1:4332174/1‑69 (MQ=255)
                 tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCg                                                      <  1:2098620/71‑1 (MQ=255)
                      acATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAAAAGAAGAGAGGAAGGCGGTGAg                                                 >  1:2566778/2‑71 (MQ=255)
                       cATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                                                                       >  1:3032727/1‑48 (MQ=255)
                         tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa                                              <  1:4671686/71‑1 (MQ=255)
                          cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt                                             >  1:606189/1‑71 (MQ=255)
                           aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                                                        >  1:2090024/1‑43 (MQ=255)
                           aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                                                        >  1:2973408/1‑43 (MQ=255)
                           aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                                                        >  1:1973001/1‑43 (MQ=255)
                           aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                                                        >  1:1108406/1‑43 (MQ=255)
                           aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagag                                                               >  1:2789313/1‑52 (MQ=255)
                           aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc                                            <  1:4929070/71‑3 (MQ=255)
                           aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc                                            <  1:3490046/71‑3 (MQ=255)
                            aTGGACAACCCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc                                            >  1:3660895/1‑70 (MQ=255)
                             tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                                                    <  1:2407353/45‑1 (MQ=255)
                             tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                                                    <  1:4363712/45‑1 (MQ=255)
                             tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                                                    <  1:3300775/45‑1 (MQ=255)
                             tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                                                    <  1:3637119/45‑1 (MQ=255)
                             tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt                                                    <  1:218872/61‑1 (MQ=255)
                               gACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt                                        <  1:3523388/71‑1 (MQ=255)
                               gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa                                                          >  1:1057806/1‑53 (MQ=255)
                                 cAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGc                                      >  1:3578976/1‑71 (MQ=255)
                                   gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg                                    <  1:3499369/70‑1 (MQ=255)
                                   gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg                                    <  1:4148179/70‑1 (MQ=255)
                                    cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                                                >  1:5598692/1‑42 (MQ=255)
                                    cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                                                >  1:800238/1‑42 (MQ=255)
                                    cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                                                >  1:2020014/1‑42 (MQ=255)
                                    cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                         >  1:1755250/1‑65 (MQ=255)
                                       accAATCAGCACCGTCGGCATATTGTTCATGACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGaac                                 <  1:4954810/69‑3 (MQ=255)
                                       accAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACg                                >  1:5332894/2‑71 (MQ=255)
                                        ccAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt                               >  1:1209201/1‑71 (MQ=255)
                                         cAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTc                              <  1:5586629/71‑1 (MQ=255)
                                         cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt                               <  1:4575829/70‑1 (MQ=255)
                                         cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTc                              >  1:5083682/1‑71 (MQ=255)
                                          aatcAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTcg                             >  1:1122560/4‑71 (MQ=255)
                                          aaCCAGTACCGTCGGCATATTGTTCATAATagaaga                                                                <  1:579353/36‑1 (MQ=255)
                                             cAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCgg                          >  1:4912015/1‑71 (MQ=255)
                                                 aCCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccc                       <  1:3247073/70‑1 (MQ=255)
                                                  ccGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCca                      <  1:2492122/70‑1 (MQ=255)
                                                   cGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcata                    <  1:86341/71‑3 (MQ=255)
                                                    gTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcata                    >  1:1935347/1‑68 (MQ=255)
                                                    gTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAg                   <  1:1573231/71‑1 (MQ=255)
                                                     tCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcataa                   >  1:4628111/1‑67 (MQ=255)
                                                      cGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa                                              <  1:1006725/42‑1 (MQ=255)
                                                       ggCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGt                               <  1:836104/56‑1 (MQ=255)
                                                       ggCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGcc                >  1:1587922/1‑71 (MQ=255)
                                                        gCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcat                     <  1:3142045/65‑2 (MQ=255)
                                                           tatTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccc                       >  1:2083290/1‑60 (MQ=255)
                                                             ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                         >  1:5356225/1‑40 (MQ=255)
                                                             ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                         >  1:5231212/1‑40 (MQ=255)
                                                             ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                         >  1:3211892/1‑40 (MQ=255)
                                                             ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATc          >  1:4249838/1‑71 (MQ=255)
                                                                ttCATAATAGAAGAGGGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTa            <  1:2715517/66‑1 (MQ=255)
                                                                     aataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCa  >  1:1830576/1‑71 (MQ=255)
                                                                     |                                                                      
GATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCA  >  Exported/2595527‑2595666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: