Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,602 A→T 36.6% S332S (TCT→TCA arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,6020AT36.6% 34.8 / 40.5 41S332S (TCT→TCAarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (17/9);  new base T (5/10);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 5.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

CGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTT  >  Exported/2595532‑2595670
                                                                      |                                                                    
cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa                                                                      <  1:464555/71‑1 (MQ=255)
cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa                                                                      <  1:3890566/71‑1 (MQ=255)
 gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaag                                                                     <  1:1003764/71‑1 (MQ=255)
  ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                                                    <  1:1625058/71‑1 (MQ=255)
     gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag                                                                 <  1:5283863/71‑1 (MQ=255)
          cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg                                                            >  1:4332174/1‑69 (MQ=255)
            tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCg                                                          <  1:2098620/71‑1 (MQ=255)
                 acATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAAAAGAAGAGAGGAAGGCGGTGAg                                                     >  1:2566778/2‑71 (MQ=255)
                    tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa                                                  <  1:4671686/71‑1 (MQ=255)
                     cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt                                                 >  1:606189/1‑71 (MQ=255)
                      aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagag                                                                   >  1:2789313/1‑52 (MQ=255)
                      aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc                                                <  1:3490046/71‑3 (MQ=255)
                      aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc                                                <  1:4929070/71‑3 (MQ=255)
                       aTGGACAACCCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc                                                >  1:3660895/1‑70 (MQ=255)
                        tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt                                                        <  1:218872/61‑1 (MQ=255)
                          gACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt                                            <  1:3523388/71‑1 (MQ=255)
                          gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa                                                              >  1:1057806/1‑53 (MQ=255)
                            cAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGc                                          >  1:3578976/1‑71 (MQ=255)
                              gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg                                        <  1:4148179/70‑1 (MQ=255)
                              gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg                                        <  1:3499369/70‑1 (MQ=255)
                               cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                                                    >  1:2020014/1‑42 (MQ=255)
                               cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                                                    >  1:5598692/1‑42 (MQ=255)
                               cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                                                                    >  1:800238/1‑42 (MQ=255)
                               cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                             >  1:1755250/1‑65 (MQ=255)
                                  accAATCAGCACCGTCGGCATATTGTTCATGACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGaac                                     <  1:4954810/69‑3 (MQ=255)
                                  accAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACg                                    >  1:5332894/2‑71 (MQ=255)
                                   ccAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt                                   >  1:1209201/1‑71 (MQ=255)
                                    cAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTc                                  <  1:5586629/71‑1 (MQ=255)
                                    cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt                                   <  1:4575829/70‑1 (MQ=255)
                                    cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTc                                  >  1:5083682/1‑71 (MQ=255)
                                     aatcAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTcg                                 >  1:1122560/4‑71 (MQ=255)
                                     aaCCAGTACCGTCGGCATATTGTTCATAATagaaga                                                                    <  1:579353/36‑1 (MQ=255)
                                        cAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCgg                              >  1:4912015/1‑71 (MQ=255)
                                            aCCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccc                           <  1:3247073/70‑1 (MQ=255)
                                             ccGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCca                          <  1:2492122/70‑1 (MQ=255)
                                              cGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcata                        <  1:86341/71‑3 (MQ=255)
                                               gTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcata                        >  1:1935347/1‑68 (MQ=255)
                                               gTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAg                       <  1:1573231/71‑1 (MQ=255)
                                                tCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcataa                       >  1:4628111/1‑67 (MQ=255)
                                                 cGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa                                                  <  1:1006725/42‑1 (MQ=255)
                                                  ggCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGt                                   <  1:836104/56‑1 (MQ=255)
                                                  ggCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGcc                    >  1:1587922/1‑71 (MQ=255)
                                                   gCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcat                         <  1:3142045/65‑2 (MQ=255)
                                                      tatTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccc                           >  1:2083290/1‑60 (MQ=255)
                                                        ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                             >  1:3211892/1‑40 (MQ=255)
                                                        ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                             >  1:5231212/1‑40 (MQ=255)
                                                        ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                             >  1:5356225/1‑40 (MQ=255)
                                                        ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATc              >  1:4249838/1‑71 (MQ=255)
                                                           ttCATAATAGAAGAGGGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTa                <  1:2715517/66‑1 (MQ=255)
                                                                aataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCa      >  1:1830576/1‑71 (MQ=255)
                                                                 ataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCAc     >  1:1467928/1‑71 (MQ=255)
                                                                     aagaGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGtt  <  1:4530913/70‑1 (MQ=255)
                                                                      |                                                                    
CGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTT  >  Exported/2595532‑2595670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: