Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,617 G→C 35.6% L327L (CTC→CTG) ‡ arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,6170GC35.6% 38.2 / 39.2 45L327L (CTC→CTG) ‡arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (17/12);  new base C (4/12);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 5.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

CCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAG  >  Exported/2595549‑2595685
                                                                    |                                                                    
acATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAAAAGAAGAGAGGAAGGCGGTGAg                                                                    >  1:2566778/2‑71 (MQ=255)
   tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa                                                                 <  1:4671686/71‑1 (MQ=255)
    cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt                                                                >  1:606189/1‑71 (MQ=255)
     aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc                                                               <  1:4929070/71‑3 (MQ=255)
     aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc                                                               <  1:3490046/71‑3 (MQ=255)
      aTGGACAACCCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc                                                               >  1:3660895/1‑70 (MQ=255)
         gACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt                                                           <  1:3523388/71‑1 (MQ=255)
           cAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGc                                                         >  1:3578976/1‑71 (MQ=255)
             gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg                                                       <  1:4148179/70‑1 (MQ=255)
             gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg                                                       <  1:3499369/70‑1 (MQ=255)
              cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                                            >  1:1755250/1‑65 (MQ=255)
                 accAATCAGCACCGTCGGCATATTGTTCATGACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGaac                                                    <  1:4954810/69‑3 (MQ=255)
                 accAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACg                                                   >  1:5332894/2‑71 (MQ=255)
                  ccAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt                                                  >  1:1209201/1‑71 (MQ=255)
                   cAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTc                                                 <  1:5586629/71‑1 (MQ=255)
                   cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt                                                  <  1:4575829/70‑1 (MQ=255)
                   cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTc                                                 >  1:5083682/1‑71 (MQ=255)
                    aatcAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTcg                                                >  1:1122560/4‑71 (MQ=255)
                       cAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCgg                                             >  1:4912015/1‑71 (MQ=255)
                           aCCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccc                                          <  1:3247073/70‑1 (MQ=255)
                            ccGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCca                                         <  1:2492122/70‑1 (MQ=255)
                             cGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcata                                       <  1:86341/71‑3 (MQ=255)
                              gTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcata                                       >  1:1935347/1‑68 (MQ=255)
                              gTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAg                                      <  1:1573231/71‑1 (MQ=255)
                               tCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcataa                                      >  1:4628111/1‑67 (MQ=255)
                                cGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa                                                                 <  1:1006725/42‑1 (MQ=255)
                                 ggCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGt                                                  <  1:836104/56‑1 (MQ=255)
                                 ggCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGcc                                   >  1:1587922/1‑71 (MQ=255)
                                  gCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcat                                        <  1:3142045/65‑2 (MQ=255)
                                     tatTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccc                                          >  1:2083290/1‑60 (MQ=255)
                                       ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                                            >  1:3211892/1‑40 (MQ=255)
                                       ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                                            >  1:5231212/1‑40 (MQ=255)
                                       ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                                            >  1:5356225/1‑40 (MQ=255)
                                       ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATc                             >  1:4249838/1‑71 (MQ=255)
                                          ttCATAATAGAAGAGGGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTa                               <  1:2715517/66‑1 (MQ=255)
                                               aataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCa                     >  1:1830576/1‑71 (MQ=255)
                                                ataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCAc                    >  1:1467928/1‑71 (MQ=255)
                                                    aagaGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGtt                 <  1:4530913/70‑1 (MQ=255)
                                                     tgagagaAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCa                     <  1:4123460/64‑1 (MQ=255)
                                                     tgagagaAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCa                     <  1:580369/64‑1 (MQ=255)
                                                      gagagGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAg              <  1:1046953/71‑1 (MQ=255)
                                                        gagGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTa            >  1:993494/1‑71 (MQ=255)
                                                                 ggTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGaaa   <  1:2801304/71‑1 (MQ=255)
                                                                  gTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAg  <  1:3897867/71‑1 (MQ=255)
                                                                  gTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAg  <  1:3026881/71‑1 (MQ=255)
                                                                    |                                                                    
CCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAG  >  Exported/2595549‑2595685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: