Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,641 G→T 23.5% A319A (GCC→GCA arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,6410GT23.5% 71.5 / 12.2 34A319A (GCC→GCAarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (11/15);  new base T (4/4);  total (15/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGC  >  Exported/2595572‑2595710
                                                                     |                                                                     
cAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCgg                                                                      >  1:4912015/1‑71 (MQ=255)
    aCCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccc                                                                   <  1:3247073/70‑1 (MQ=255)
     ccGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCca                                                                  <  1:2492122/70‑1 (MQ=255)
      cGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcata                                                                <  1:86341/71‑3 (MQ=255)
       gTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcata                                                                >  1:1935347/1‑68 (MQ=255)
       gTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAg                                                               <  1:1573231/71‑1 (MQ=255)
        tCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcataa                                                               >  1:4628111/1‑67 (MQ=255)
          ggCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGcc                                                            >  1:1587922/1‑71 (MQ=255)
           gCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcat                                                                 <  1:3142045/65‑2 (MQ=255)
              tatTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccc                                                                   >  1:2083290/1‑60 (MQ=255)
                ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATc                                                      >  1:4249838/1‑71 (MQ=255)
                   ttCATAATAGAAGAGGGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTa                                                        <  1:2715517/66‑1 (MQ=255)
                        aataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCa                                              >  1:1830576/1‑71 (MQ=255)
                         ataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCAc                                             >  1:1467928/1‑71 (MQ=255)
                             aagaGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGtt                                          <  1:4530913/70‑1 (MQ=255)
                              tgagagaAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCa                                              <  1:580369/64‑1 (MQ=255)
                              tgagagaAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCa                                              <  1:4123460/64‑1 (MQ=255)
                               gagagGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAg                                       <  1:1046953/71‑1 (MQ=255)
                                 gagGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTa                                     >  1:993494/1‑71 (MQ=255)
                                          ggTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGaaa                            <  1:2801304/71‑1 (MQ=255)
                                           gTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAg                           <  1:3897867/71‑1 (MQ=255)
                                           gTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAg                           <  1:3026881/71‑1 (MQ=255)
                                              aagAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCAGATTCAGCACGCCAGACAGgta                        >  1:373104/3‑71 (MQ=255)
                                              aagAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCAGATTCAGCACGCCAGACAGgta                        >  1:3969711/3‑71 (MQ=255)
                                              aGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACg                                            <  1:4723700/51‑1 (MQ=255)
                                                  aaccGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTcc                    <  1:3970417/69‑1 (MQ=255)
                                                  aTCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTcc                    <  1:2700788/71‑1 (MQ=255)
                                                   tCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGaaa                            >  1:4175024/1‑62 (MQ=255)
                                                   tCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGata                        >  1:4961856/1‑66 (MQ=255)
                                                    ccGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATc                                                      >  1:3653529/1‑35 (MQ=255)
                                                    ccGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATc                                                      >  1:3724830/1‑35 (MQ=255)
                                                    ccGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATc                                                      >  1:3994860/1‑35 (MQ=255)
                                                       gTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTaa               <  1:153207/71‑1 (MQ=255)
                                                          ccGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATcc            <  1:5288169/71‑1 (MQ=255)
                                                                 tCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg          <  1:2943725/66‑1 (MQ=255)
                                                                   gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg   >  1:1999490/1‑71 (MQ=255)
                                                                   gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg   >  1:981857/1‑71 (MQ=255)
                                                                   gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg   >  1:3402723/1‑71 (MQ=255)
                                                                    cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGaaa                            <  1:3236981/45‑1 (MQ=255)
                                                                    cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTa                <  1:34383/57‑1 (MQ=255)
                                                                    cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTa                <  1:2347561/57‑1 (MQ=255)
                                                                    cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcgc  <  1:3485629/71‑1 (MQ=255)
                                                                     ggCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg   >  1:3567205/1‑69 (MQ=255)
                                                                     |                                                                     
CAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGC  >  Exported/2595572‑2595710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: