Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,683 A→C 10.3% L305L (CTT→CTG arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,6830AC10.3% 226.5 / 21.3 97L305L (CTT→CTGarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (47/40);  new base C (5/5);  total (52/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 103 total aligned reads displayed.

GGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGA  >  Exported/2595614‑2595750
                                                                     |                                                                   
ggTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGaaa                                                                    <  1:2801304/71‑1 (MQ=255)
 gTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAg                                                                   <  1:3897867/71‑1 (MQ=255)
 gTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAg                                                                   <  1:3026881/71‑1 (MQ=255)
    aagAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCAGATTCAGCACGCCAGACAGgta                                                                >  1:3969711/3‑71 (MQ=255)
    aagAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCAGATTCAGCACGCCAGACAGgta                                                                >  1:373104/3‑71 (MQ=255)
        aaccGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTcc                                                            <  1:3970417/69‑1 (MQ=255)
        aTCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTcc                                                            <  1:2700788/71‑1 (MQ=255)
         tCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGaaa                                                                    >  1:4175024/1‑62 (MQ=255)
         tCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGata                                                                >  1:4961856/1‑66 (MQ=255)
             gTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTaa                                                       <  1:153207/71‑1 (MQ=255)
                ccGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATcc                                                    <  1:5288169/71‑1 (MQ=255)
                       tCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                  <  1:2943725/66‑1 (MQ=255)
                         gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                           >  1:3402723/1‑71 (MQ=255)
                         gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                           >  1:1999490/1‑71 (MQ=255)
                          cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGaaa                                                                    <  1:3236981/45‑1 (MQ=255)
                          cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTa                                                        <  1:34383/57‑1 (MQ=255)
                          cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTa                                                        <  1:2347561/57‑1 (MQ=255)
                          cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcgc                                          <  1:3485629/71‑1 (MQ=255)
                           ggCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                           >  1:3567205/1‑69 (MQ=255)
                            gCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCAtt                                              <  1:2292966/65‑1 (MQ=255)
                              ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGatat                                                               <  1:1465895/46‑1 (MQ=255)
                              ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgc                                            >  1:3961938/1‑65 (MQ=255)
                              ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                      >  1:5602778/1‑71 (MQ=255)
                              ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                      >  1:2526325/1‑71 (MQ=255)
                              ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                      >  1:3780043/1‑71 (MQ=255)
                               cacaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                      >  1:4903128/1‑70 (MQ=255)
                                acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTaa                                                       <  1:5096946/52‑1 (MQ=255)
                                acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                    >  1:1896900/1‑71 (MQ=255)
                                acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                    >  1:4126379/1‑71 (MQ=255)
                                acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                    >  1:4029242/1‑71 (MQ=255)
                                acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                    >  1:2682705/1‑71 (MQ=255)
                                      cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGa                                                                  <  1:1618024/35‑1 (MQ=255)
                                      cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCg                                                           >  1:4684057/1‑42 (MQ=255)
                                      cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCg                                                           >  1:1189166/1‑42 (MQ=255)
                                      cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCg                                                           >  1:3618126/1‑42 (MQ=255)
                                      cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                              <  1:5526257/71‑1 (MQ=255)
                                        ttATCCGCCAGCACGTTGAGTACGCCAGAAAGata                                                                <  1:3502368/35‑1 (MQ=255)
                                        ttATCCGCCAGCACGTTGAGTACGCCAGAAAGata                                                                <  1:2678556/35‑1 (MQ=255)
                                        ttATCCGCCAGCACGTTGAGTACGCCAGAAAGata                                                                <  1:1857714/35‑1 (MQ=255)
                                        ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                  <  1:5383454/49‑1 (MQ=255)
                                        ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                  <  1:5618776/49‑1 (MQ=255)
                                        ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                  <  1:2278032/49‑1 (MQ=255)
                                        ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                  <  1:127529/49‑1 (MQ=255)
                                        ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaaa                                <  1:4828598/67‑1 (MQ=255)
                                         tATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                            <  1:2842828/70‑1 (MQ=255)
                                          aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCa                                                >  1:4861178/1‑49 (MQ=255)
                                          aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCa                                                >  1:1257627/1‑49 (MQ=255)
                                          aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAgg                                       >  1:2411165/1‑58 (MQ=255)
                                          aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                            >  1:1762316/1‑69 (MQ=255)
                                          aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                            >  1:4305840/1‑69 (MQ=255)
                                          aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                            >  1:2871876/1‑69 (MQ=255)
                                          aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                             >  1:5442086/1‑68 (MQ=255)
                                          aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                             >  1:1326877/1‑68 (MQ=255)
                                          aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                             >  1:2537540/1‑68 (MQ=255)
                                          aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                              >  1:4164990/1‑67 (MQ=255)
                                          aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                              >  1:2400230/1‑67 (MQ=255)
                                          aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                               >  1:1798524/1‑66 (MQ=255)
                                            ctgCCAGCAGATTCAGCACGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATa                                  >  1:5314827/3‑61 (MQ=255)
                                            ctgCCAGCAGATTCAGCACGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATa                                  >  1:3648782/3‑61 (MQ=255)
                                             cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                        <  1:237230/70‑1 (MQ=255)
                                             cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                        <  1:2578486/70‑1 (MQ=255)
                                             cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                        <  1:5487768/70‑1 (MQ=255)
                                             cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt                       <  1:394464/71‑1 (MQ=255)
                                             cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt                       <  1:4913772/71‑1 (MQ=255)
                                               ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAAt                                                      <  1:1728665/38‑1 (MQ=255)
                                               ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAAt                                                      <  1:4727417/38‑1 (MQ=255)
                                               ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATa                      <  1:5692575/70‑1 (MQ=255)
                                                cAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATc                                                     >  1:2163570/1‑38 (MQ=255)
                                                cAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATc                                                     >  1:3740996/1‑38 (MQ=255)
                                                 aGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACAt                   >  1:619326/1‑71 (MQ=255)
                                                     cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg               <  1:4507846/71‑1 (MQ=255)
                                                     cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg               <  1:2684351/71‑1 (MQ=255)
                                                      gTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                  <  1:913053/35‑1 (MQ=255)
                                                      gTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                  <  1:2980581/35‑1 (MQ=255)
                                                       ttGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATa                                  <  1:2925855/50‑1 (MQ=255)
                                                        tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca                          >  1:1491589/1‑57 (MQ=255)
                                                        tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc            >  1:1370406/1‑71 (MQ=255)
                                                         gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                           >  1:564529/1‑39 (MQ=255)
                                                         gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                           >  1:53938/1‑39 (MQ=255)
                                                         gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                               >  1:5452169/1‑51 (MQ=255)
                                                         gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                               >  1:962069/1‑51 (MQ=255)
                                                             aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa       <  1:3268683/71‑1 (MQ=255)
                                                             aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa       <  1:434414/71‑1 (MQ=255)
                                                             aCGCCAGAAAGATATTCCTTTAATCCAGCATTGCGCAGGCCATaaa                                >  1:4252219/1‑46 (MQ=255)
                                                             aCGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaaa                                >  1:3645637/1‑46 (MQ=255)
                                                             aCGACAGAAAGATATTCCGTTAATCCGGCATTGAGCAGGCCATaaa                                >  1:545180/1‑46 (MQ=39)
                                                              cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaa                                 >  1:40940/1‑44 (MQ=255)
                                                              cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag         >  1:3286343/1‑68 (MQ=255)
                                                               gCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaaaa     <  1:951590/71‑3 (MQ=255)
                                                                ccAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt    <  1:328670/71‑1 (MQ=255)
                                                                ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                            >  1:1359151/1‑47 (MQ=255)
                                                                ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                            >  1:3714069/1‑47 (MQ=255)
                                                                ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt    <  1:5096957/71‑1 (MQ=255)
                                                                ccAGAAAGATATTCCGTTAATCCGGAATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga     >  1:4739359/1‑70 (MQ=255)
                                                                 cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt    <  1:3390875/70‑1 (MQ=255)
                                                                  aGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGa  <  1:3853428/71‑1 (MQ=255)
                                                                   gAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaa                                 <  1:885608/39‑1 (MQ=255)
                                                                    aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa       >  1:3888093/1‑64 (MQ=255)
                                                                    aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa       >  1:5593260/1‑64 (MQ=255)
                                                                    aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa       >  1:1902425/1‑64 (MQ=255)
                                                                     |                                                                   
GGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGA  >  Exported/2595614‑2595750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: