Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,686 A→G 8.8% Y304Y (TAT→TAC arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,6860AG 8.8% 249.9 / 11.2 91Y304Y (TAT→TACarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (50/33);  new base G (3/5);  total (53/38)
Fisher's exact test for biased strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

AGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAT  >  Exported/2595618‑2595754
                                                                    |                                                                    
aagAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCAGATTCAGCACGCCAGACAGgta                                                                    >  1:3969711/3‑71 (MQ=255)
aagAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCAGATTCAGCACGCCAGACAGgta                                                                    >  1:373104/3‑71 (MQ=255)
    aaccGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTcc                                                                <  1:3970417/69‑1 (MQ=255)
    aTCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTcc                                                                <  1:2700788/71‑1 (MQ=255)
     tCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGata                                                                    >  1:4961856/1‑66 (MQ=255)
         gTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTaa                                                           <  1:153207/71‑1 (MQ=255)
            ccGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATcc                                                        <  1:5288169/71‑1 (MQ=255)
                   tCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                      <  1:2943725/66‑1 (MQ=255)
                     gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                               >  1:3402723/1‑71 (MQ=255)
                     gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                               >  1:981857/1‑71 (MQ=255)
                     gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                               >  1:1999490/1‑71 (MQ=255)
                      cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTa                                                            <  1:34383/57‑1 (MQ=255)
                      cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTa                                                            <  1:2347561/57‑1 (MQ=255)
                      cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcgc                                              <  1:3485629/71‑1 (MQ=255)
                       ggCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                               >  1:3567205/1‑69 (MQ=255)
                        gCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCAtt                                                  <  1:2292966/65‑1 (MQ=255)
                          ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGatat                                                                   <  1:1465895/46‑1 (MQ=255)
                          ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgc                                                >  1:3961938/1‑65 (MQ=255)
                          ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                          >  1:3780043/1‑71 (MQ=255)
                          ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                          >  1:2526325/1‑71 (MQ=255)
                          ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                          >  1:5602778/1‑71 (MQ=255)
                           cacaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                          >  1:4903128/1‑70 (MQ=255)
                            acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTaa                                                           <  1:5096946/52‑1 (MQ=255)
                            acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                        >  1:1896900/1‑71 (MQ=255)
                            acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                        >  1:2875794/1‑71 (MQ=255)
                            acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                        >  1:4126379/1‑71 (MQ=255)
                            acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                        >  1:4029242/1‑71 (MQ=255)
                            acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                        >  1:2682705/1‑71 (MQ=255)
                                  cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGa                                                                      <  1:1618024/35‑1 (MQ=255)
                                  cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCg                                                               >  1:1189166/1‑42 (MQ=255)
                                  cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCg                                                               >  1:3618126/1‑42 (MQ=255)
                                  cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCg                                                               >  1:4684057/1‑42 (MQ=255)
                                  cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                                  <  1:5526257/71‑1 (MQ=255)
                                    ttATCCGCCAGCACGTTGAGTACGCCAGAAAGata                                                                    <  1:2678556/35‑1 (MQ=255)
                                    ttATCCGCCAGCACGTTGAGTACGCCAGAAAGata                                                                    <  1:1857714/35‑1 (MQ=255)
                                    ttATCCGCCAGCACGTTGAGTACGCCAGAAAGata                                                                    <  1:3502368/35‑1 (MQ=255)
                                    ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                      <  1:2278032/49‑1 (MQ=255)
                                    ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                      <  1:5383454/49‑1 (MQ=255)
                                    ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                      <  1:5618776/49‑1 (MQ=255)
                                    ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                      <  1:127529/49‑1 (MQ=255)
                                    ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaaa                                    <  1:4828598/67‑1 (MQ=255)
                                     tATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                <  1:2842828/70‑1 (MQ=255)
                                      aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCa                                                    >  1:4861178/1‑49 (MQ=255)
                                      aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCa                                                    >  1:1257627/1‑49 (MQ=255)
                                      aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAgg                                           >  1:2411165/1‑58 (MQ=255)
                                      aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                >  1:1762316/1‑69 (MQ=255)
                                      aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                >  1:4305840/1‑69 (MQ=255)
                                      aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                >  1:2871876/1‑69 (MQ=255)
                                      aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                                 >  1:1326877/1‑68 (MQ=255)
                                      aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                                 >  1:5442086/1‑68 (MQ=255)
                                      aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                                 >  1:2537540/1‑68 (MQ=255)
                                      aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                                  >  1:2400230/1‑67 (MQ=255)
                                      aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                                  >  1:4164990/1‑67 (MQ=255)
                                      aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                                   >  1:1798524/1‑66 (MQ=255)
                                        ctgCCAGCAGATTCAGCACGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATa                                      >  1:5314827/3‑61 (MQ=255)
                                        ctgCCAGCAGATTCAGCACGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATa                                      >  1:3648782/3‑61 (MQ=255)
                                         cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                            <  1:5487768/70‑1 (MQ=255)
                                         cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                            <  1:2578486/70‑1 (MQ=255)
                                         cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                            <  1:237230/70‑1 (MQ=255)
                                         cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt                           <  1:394464/71‑1 (MQ=255)
                                         cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt                           <  1:4913772/71‑1 (MQ=255)
                                           ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAAt                                                          <  1:1728665/38‑1 (MQ=255)
                                           ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAAt                                                          <  1:4727417/38‑1 (MQ=255)
                                           ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATa                          <  1:5692575/70‑1 (MQ=255)
                                            cAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATc                                                         >  1:3740996/1‑38 (MQ=255)
                                            cAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATc                                                         >  1:2163570/1‑38 (MQ=255)
                                             aGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACAt                       >  1:619326/1‑71 (MQ=255)
                                                 cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg                   <  1:4507846/71‑1 (MQ=255)
                                                 cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg                   <  1:2684351/71‑1 (MQ=255)
                                                  gTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                      <  1:2980581/35‑1 (MQ=255)
                                                  gTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                      <  1:913053/35‑1 (MQ=255)
                                                   ttGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATa                                      <  1:2925855/50‑1 (MQ=255)
                                                    tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca                              >  1:1491589/1‑57 (MQ=255)
                                                    tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc                >  1:1370406/1‑71 (MQ=255)
                                                     gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                               >  1:53938/1‑39 (MQ=255)
                                                     gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                               >  1:564529/1‑39 (MQ=255)
                                                     gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                                   >  1:962069/1‑51 (MQ=255)
                                                     gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                                   >  1:5452169/1‑51 (MQ=255)
                                                         aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa           <  1:3268683/71‑1 (MQ=255)
                                                         aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa           <  1:434414/71‑1 (MQ=255)
                                                         aCGCCAGAAAGATATTCCTTTAATCCAGCATTGCGCAGGCCATaaa                                    >  1:4252219/1‑46 (MQ=255)
                                                         aCGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaaa                                    >  1:3645637/1‑46 (MQ=255)
                                                         aCGACAGAAAGATATTCCGTTAATCCGGCATTGAGCAGGCCATaaa                                    >  1:545180/1‑46 (MQ=39)
                                                          cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaa                                     >  1:40940/1‑44 (MQ=255)
                                                          cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag             >  1:3286343/1‑68 (MQ=255)
                                                           gCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaaaa         <  1:951590/71‑3 (MQ=255)
                                                            ccAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt        <  1:328670/71‑1 (MQ=255)
                                                            ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                >  1:1359151/1‑47 (MQ=255)
                                                            ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                >  1:3714069/1‑47 (MQ=255)
                                                            ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt        <  1:5096957/71‑1 (MQ=255)
                                                            ccAGAAAGATATTCCGTTAATCCGGAATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga         >  1:4739359/1‑70 (MQ=255)
                                                             cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt        >  1:4179180/1‑70 (MQ=255)
                                                             cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt        <  1:3390875/70‑1 (MQ=255)
                                                              aGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGa      <  1:3853428/71‑1 (MQ=255)
                                                               gAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaa                                     <  1:885608/39‑1 (MQ=255)
                                                                aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa           >  1:1902425/1‑64 (MQ=255)
                                                                aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa           >  1:5593260/1‑64 (MQ=255)
                                                                aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa           >  1:3888093/1‑64 (MQ=255)
                                                                  aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt  >  1:5290268/1‑71 (MQ=255)
                                                                    atatTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag          <  1:3366957/61‑1 (MQ=255)
                                                                    |                                                                    
AGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAT  >  Exported/2595618‑2595754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: