Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,689 T→C 8.8% E303E (GAA→GAG arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,6890TC 8.8% 249.2 / 11.2 91E303E (GAA→GAGarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base T (50/33);  new base C (3/5);  total (53/38)
Fisher's exact test for biased strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

ATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCC  >  Exported/2595622‑2595759
                                                                   |                                                                      
aaccGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTcc                                                                     <  1:3970417/69‑1 (MQ=255)
aTCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTcc                                                                     <  1:2700788/71‑1 (MQ=255)
     gTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTaa                                                                <  1:153207/71‑1 (MQ=255)
        ccGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATcc                                                             <  1:5288169/71‑1 (MQ=255)
               tCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                           <  1:2943725/66‑1 (MQ=255)
                 gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                    >  1:3402723/1‑71 (MQ=255)
                 gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                    >  1:981857/1‑71 (MQ=255)
                 gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                    >  1:1999490/1‑71 (MQ=255)
                  cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTa                                                                 <  1:2347561/57‑1 (MQ=255)
                  cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTa                                                                 <  1:34383/57‑1 (MQ=255)
                  cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcgc                                                   <  1:3485629/71‑1 (MQ=255)
                   ggCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                    >  1:3567205/1‑69 (MQ=255)
                    gCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCAtt                                                       <  1:2292966/65‑1 (MQ=255)
                      ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGatat                                                                        <  1:1465895/46‑1 (MQ=255)
                      ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgc                                                     >  1:3961938/1‑65 (MQ=255)
                      ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                               >  1:5602778/1‑71 (MQ=255)
                      ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                               >  1:3780043/1‑71 (MQ=255)
                      ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                               >  1:2526325/1‑71 (MQ=255)
                       cacaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                               >  1:4903128/1‑70 (MQ=255)
                        acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTaa                                                                <  1:5096946/52‑1 (MQ=255)
                        acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                             >  1:4126379/1‑71 (MQ=255)
                        acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                             >  1:4029242/1‑71 (MQ=255)
                        acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                             >  1:1896900/1‑71 (MQ=255)
                        acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                             >  1:2875794/1‑71 (MQ=255)
                        acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                             >  1:2682705/1‑71 (MQ=255)
                              cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCg                                                                    >  1:1189166/1‑42 (MQ=255)
                              cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCg                                                                    >  1:4684057/1‑42 (MQ=255)
                              cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCg                                                                    >  1:3618126/1‑42 (MQ=255)
                              cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                                       <  1:5526257/71‑1 (MQ=255)
                                ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                           <  1:2278032/49‑1 (MQ=255)
                                ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                           <  1:5618776/49‑1 (MQ=255)
                                ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                           <  1:5383454/49‑1 (MQ=255)
                                ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                           <  1:127529/49‑1 (MQ=255)
                                ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaaa                                         <  1:4828598/67‑1 (MQ=255)
                                 tATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                     <  1:2842828/70‑1 (MQ=255)
                                  aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCa                                                         >  1:4861178/1‑49 (MQ=255)
                                  aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCa                                                         >  1:1257627/1‑49 (MQ=255)
                                  aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAgg                                                >  1:2411165/1‑58 (MQ=255)
                                  aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                     >  1:4305840/1‑69 (MQ=255)
                                  aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                     >  1:2871876/1‑69 (MQ=255)
                                  aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                     >  1:1762316/1‑69 (MQ=255)
                                  aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                                      >  1:1326877/1‑68 (MQ=255)
                                  aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                                      >  1:2537540/1‑68 (MQ=255)
                                  aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                                      >  1:5442086/1‑68 (MQ=255)
                                  aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                                       >  1:2400230/1‑67 (MQ=255)
                                  aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                                       >  1:4164990/1‑67 (MQ=255)
                                  aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                                        >  1:1798524/1‑66 (MQ=255)
                                    ctgCCAGCAGATTCAGCACGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATa                                           >  1:5314827/3‑61 (MQ=255)
                                    ctgCCAGCAGATTCAGCACGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATa                                           >  1:3648782/3‑61 (MQ=255)
                                     cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                 <  1:237230/70‑1 (MQ=255)
                                     cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                 <  1:5487768/70‑1 (MQ=255)
                                     cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                 <  1:2578486/70‑1 (MQ=255)
                                     cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt                                <  1:4913772/71‑1 (MQ=255)
                                     cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt                                <  1:394464/71‑1 (MQ=255)
                                       ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAAt                                                               <  1:1728665/38‑1 (MQ=255)
                                       ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAAt                                                               <  1:4727417/38‑1 (MQ=255)
                                       ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATa                               <  1:5692575/70‑1 (MQ=255)
                                        cAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATc                                                              >  1:2163570/1‑38 (MQ=255)
                                        cAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATc                                                              >  1:3740996/1‑38 (MQ=255)
                                         aGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACAt                            >  1:619326/1‑71 (MQ=255)
                                             cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg                        <  1:2684351/71‑1 (MQ=255)
                                             cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg                        <  1:4507846/71‑1 (MQ=255)
                                              gTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                           <  1:2980581/35‑1 (MQ=255)
                                              gTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                           <  1:913053/35‑1 (MQ=255)
                                               ttGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATa                                           <  1:2925855/50‑1 (MQ=255)
                                                tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca                                   >  1:1491589/1‑57 (MQ=255)
                                                tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc                     >  1:1370406/1‑71 (MQ=255)
                                                 gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                    >  1:564529/1‑39 (MQ=255)
                                                 gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                    >  1:53938/1‑39 (MQ=255)
                                                 gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                                        >  1:962069/1‑51 (MQ=255)
                                                 gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                                        >  1:5452169/1‑51 (MQ=255)
                                                     aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa                <  1:434414/71‑1 (MQ=255)
                                                     aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa                <  1:3268683/71‑1 (MQ=255)
                                                     aCGCCAGAAAGATATTCCTTTAATCCAGCATTGCGCAGGCCATaaa                                         >  1:4252219/1‑46 (MQ=255)
                                                     aCGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaaa                                         >  1:3645637/1‑46 (MQ=255)
                                                     aCGACAGAAAGATATTCCGTTAATCCGGCATTGAGCAGGCCATaaa                                         >  1:545180/1‑46 (MQ=39)
                                                      cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaa                                          >  1:40940/1‑44 (MQ=255)
                                                      cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag                  >  1:3286343/1‑68 (MQ=255)
                                                       gCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaaaa              <  1:951590/71‑3 (MQ=255)
                                                        ccAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt             <  1:328670/71‑1 (MQ=255)
                                                        ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                     >  1:1359151/1‑47 (MQ=255)
                                                        ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                     >  1:3714069/1‑47 (MQ=255)
                                                        ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt             <  1:5096957/71‑1 (MQ=255)
                                                        ccAGAAAGATATTCCGTTAATCCGGAATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga              >  1:4739359/1‑70 (MQ=255)
                                                         cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt             <  1:3390875/70‑1 (MQ=255)
                                                         cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt             >  1:4179180/1‑70 (MQ=255)
                                                          aGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGa           <  1:3853428/71‑1 (MQ=255)
                                                           gAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaa                                          <  1:885608/39‑1 (MQ=255)
                                                            aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                >  1:1902425/1‑64 (MQ=255)
                                                            aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                >  1:5593260/1‑64 (MQ=255)
                                                            aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                >  1:3888093/1‑64 (MQ=255)
                                                              aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt       >  1:5290268/1‑71 (MQ=255)
                                                                atatTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag               <  1:3366957/61‑1 (MQ=255)
                                                                  atTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt             >  1:4202109/1‑61 (MQ=255)
                                                                   ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc                     <  1:343597/52‑1 (MQ=255)
                                                                   ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc  <  1:5425102/71‑1 (MQ=255)
                                                                   ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc  <  1:2489466/71‑1 (MQ=255)
                                                                   ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc   <  1:639752/70‑1 (MQ=255)
                                                                   ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc   <  1:3432388/70‑1 (MQ=255)
                                                                   |                                                                      
ATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCC  >  Exported/2595622‑2595759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: