Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,695 T→G 10.0% L301F (TTA→TTC arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,6950TG10.0% 236.0 / 21.1 100L301F (TTA→TTCarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base T (52/38);  new base G (4/6);  total (56/44)
Fisher's exact test for biased strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 103 total aligned reads displayed.

GTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGG  >  Exported/2595627‑2595765
                                                                    |                                                                      
gTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTaa                                                                      <  1:153207/71‑1 (MQ=255)
   ccGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATcc                                                                   <  1:5288169/71‑1 (MQ=255)
          tCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                                 <  1:2943725/66‑1 (MQ=255)
            gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                          >  1:981857/1‑71 (MQ=255)
            gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                          >  1:3402723/1‑71 (MQ=255)
            gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                          >  1:1999490/1‑71 (MQ=255)
             cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTa                                                                       <  1:2347561/57‑1 (MQ=255)
             cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTa                                                                       <  1:34383/57‑1 (MQ=255)
             cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcgc                                                         <  1:3485629/71‑1 (MQ=255)
              ggCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                          >  1:3567205/1‑69 (MQ=255)
               gCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCAtt                                                             <  1:2292966/65‑1 (MQ=255)
                 ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgc                                                           >  1:3961938/1‑65 (MQ=255)
                 ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                                     >  1:2526325/1‑71 (MQ=255)
                 ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                                     >  1:3780043/1‑71 (MQ=255)
                 ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                                     >  1:5602778/1‑71 (MQ=255)
                  cacaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                                     >  1:4903128/1‑70 (MQ=255)
                   acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTaa                                                                      <  1:5096946/52‑1 (MQ=255)
                   acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                                   >  1:1896900/1‑71 (MQ=255)
                   acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                                   >  1:4029242/1‑71 (MQ=255)
                   acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                                   >  1:4126379/1‑71 (MQ=255)
                   acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                                   >  1:2875794/1‑71 (MQ=255)
                   acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                                   >  1:2682705/1‑71 (MQ=255)
                         cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                                             <  1:5526257/71‑1 (MQ=255)
                           ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                                 <  1:5383454/49‑1 (MQ=255)
                           ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                                 <  1:5618776/49‑1 (MQ=255)
                           ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                                 <  1:127529/49‑1 (MQ=255)
                           ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                                 <  1:2278032/49‑1 (MQ=255)
                           ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaaa                                               <  1:4828598/67‑1 (MQ=255)
                            tATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                           <  1:2842828/70‑1 (MQ=255)
                             aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCa                                                               >  1:1257627/1‑49 (MQ=255)
                             aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCa                                                               >  1:4861178/1‑49 (MQ=255)
                             aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAgg                                                      >  1:2411165/1‑58 (MQ=255)
                             aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                           >  1:2871876/1‑69 (MQ=255)
                             aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                           >  1:4305840/1‑69 (MQ=255)
                             aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                           >  1:1762316/1‑69 (MQ=255)
                             aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                                            >  1:2537540/1‑68 (MQ=255)
                             aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                                            >  1:1326877/1‑68 (MQ=255)
                             aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                                            >  1:5442086/1‑68 (MQ=255)
                             aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                                             >  1:2400230/1‑67 (MQ=255)
                             aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                                             >  1:4164990/1‑67 (MQ=255)
                             aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                                              >  1:1798524/1‑66 (MQ=255)
                               ctgCCAGCAGATTCAGCACGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATa                                                 >  1:5314827/3‑61 (MQ=255)
                               ctgCCAGCAGATTCAGCACGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATa                                                 >  1:3648782/3‑61 (MQ=255)
                                cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                       <  1:5487768/70‑1 (MQ=255)
                                cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                       <  1:2578486/70‑1 (MQ=255)
                                cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                       <  1:237230/70‑1 (MQ=255)
                                cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt                                      <  1:394464/71‑1 (MQ=255)
                                cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt                                      <  1:4913772/71‑1 (MQ=255)
                                  ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAAt                                                                     <  1:1728665/38‑1 (MQ=255)
                                  ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAAt                                                                     <  1:4727417/38‑1 (MQ=255)
                                  ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATa                                     <  1:5692575/70‑1 (MQ=255)
                                   cAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATc                                                                    >  1:2163570/1‑38 (MQ=255)
                                   cAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATc                                                                    >  1:3740996/1‑38 (MQ=255)
                                    aGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACAt                                  >  1:619326/1‑71 (MQ=255)
                                        cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg                              <  1:4507846/71‑1 (MQ=255)
                                        cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg                              <  1:2684351/71‑1 (MQ=255)
                                         gTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                                 <  1:913053/35‑1 (MQ=255)
                                         gTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                                 <  1:2980581/35‑1 (MQ=255)
                                           tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca                                         >  1:1491589/1‑57 (MQ=255)
                                           tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc                           >  1:1370406/1‑71 (MQ=255)
                                            gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                          >  1:564529/1‑39 (MQ=255)
                                            gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                          >  1:53938/1‑39 (MQ=255)
                                            gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                                              >  1:5452169/1‑51 (MQ=255)
                                            gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                                              >  1:962069/1‑51 (MQ=255)
                                                aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa                      <  1:3268683/71‑1 (MQ=255)
                                                aCGCCAGAAAGATATTCCTTTAATCCAGCATTGCGCAGGCCATaaa                                               >  1:4252219/1‑46 (MQ=255)
                                                aCGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaaa                                               >  1:3645637/1‑46 (MQ=255)
                                                aCGACAGAAAGATATTCCGTTAATCCGGCATTGAGCAGGCCATaaa                                               >  1:545180/1‑46 (MQ=39)
                                                 cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaa                                                >  1:40940/1‑44 (MQ=255)
                                                 cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag                        >  1:3286343/1‑68 (MQ=255)
                                                  gCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaaaa                    <  1:951590/71‑3 (MQ=255)
                                                   ccAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                   <  1:328670/71‑1 (MQ=255)
                                                   ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                           >  1:1359151/1‑47 (MQ=255)
                                                   ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                           >  1:3714069/1‑47 (MQ=255)
                                                   ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                   <  1:5096957/71‑1 (MQ=255)
                                                   ccAGAAAGATATTCCGTTAATCCGGAATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga                    >  1:4739359/1‑70 (MQ=255)
                                                    cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                   <  1:3390875/70‑1 (MQ=255)
                                                    cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                   >  1:4179180/1‑70 (MQ=255)
                                                     aGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGa                 <  1:3853428/71‑1 (MQ=255)
                                                      gAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaa                                                <  1:885608/39‑1 (MQ=255)
                                                       aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                      >  1:1902425/1‑64 (MQ=255)
                                                       aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                      >  1:3888093/1‑64 (MQ=255)
                                                       aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                      >  1:5593260/1‑64 (MQ=255)
                                                         aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt             >  1:5290268/1‑71 (MQ=255)
                                                           atatTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag                     <  1:3366957/61‑1 (MQ=255)
                                                             atTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                   >  1:4202109/1‑61 (MQ=255)
                                                              ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc                           <  1:343597/52‑1 (MQ=255)
                                                              ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc        <  1:2489466/71‑1 (MQ=255)
                                                              ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc        <  1:5425102/71‑1 (MQ=255)
                                                              ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc         <  1:3432388/70‑1 (MQ=255)
                                                              ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc         <  1:639752/70‑1 (MQ=255)
                                                               tCCGTTAATCCGGCATTGCGCAGGCCATAAaccacc                                          >  1:1406758/1‑36 (MQ=255)
                                                               tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc         >  1:4215380/1‑69 (MQ=255)
                                                                ccGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg      >  1:3361955/1‑71 (MQ=255)
                                                                ccGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGcc                               <  1:1361447/46‑1 (MQ=255)
                                                                ccGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGc         >  1:1502745/1‑68 (MQ=255)
                                                                 cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga                    >  1:3009797/1‑56 (MQ=255)
                                                                 cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa       <  1:2437817/69‑1 (MQ=255)
                                                                   ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag                        <  1:5134430/50‑1 (MQ=255)
                                                                    tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg  >  1:2040723/1‑71 (MQ=255)
                                                                    |                                                                      
GTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGG  >  Exported/2595627‑2595765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: