Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,719 A→G 30.0% V293V (GTT→GTC arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,7190AG30.0% 168.6 / 97.2 130V293V (GTT→GTCarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (46/45);  new base G (18/21);  total (64/66)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

CGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCC  >  Exported/2595652‑2595785
                                                                   |                                                                  
cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                                                                 <  1:5526257/71‑1 (MQ=255)
  ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaaa                                                                   <  1:4828598/67‑1 (MQ=255)
   tATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                                               <  1:2842828/70‑1 (MQ=255)
    aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                                               >  1:2871876/1‑69 (MQ=255)
    aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                                               >  1:4305840/1‑69 (MQ=255)
    aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                                                                >  1:2537540/1‑68 (MQ=255)
    aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                                                                >  1:5442086/1‑68 (MQ=255)
    aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                                                                 >  1:2400230/1‑67 (MQ=255)
    aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                                                                 >  1:4164990/1‑67 (MQ=255)
       cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                                           <  1:5487768/70‑1 (MQ=255)
       cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                                           <  1:2578486/70‑1 (MQ=255)
       cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt                                                          <  1:4913772/71‑1 (MQ=255)
       cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt                                                          <  1:394464/71‑1 (MQ=255)
         ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATa                                                         <  1:5692575/70‑1 (MQ=255)
               cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg                                                  <  1:4507846/71‑1 (MQ=255)
               cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg                                                  <  1:2684351/71‑1 (MQ=255)
                  tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc                                               >  1:1370406/1‑71 (MQ=255)
                   gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                                                                  >  1:962069/1‑51 (MQ=255)
                   gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                                                                  >  1:5452169/1‑51 (MQ=255)
                       aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa                                          <  1:3268683/71‑1 (MQ=255)
                       aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa                                          <  1:434414/71‑1 (MQ=255)
                       aCGCCAGAAAGATATTCCTTTAATCCAGCATTGCGCAGGCCATaaa                                                                   >  1:4252219/1‑46 (MQ=255)
                       aCGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaaa                                                                   >  1:3645637/1‑46 (MQ=255)
                       aCGACAGAAAGATATTCCGTTAATCCGGCATTGAGCAGGCCATaaa                                                                   >  1:545180/1‑46 (MQ=39)
                         gCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaaaa                                        <  1:951590/71‑3 (MQ=255)
                          ccAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                                       <  1:328670/71‑1 (MQ=255)
                          ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                                               >  1:3714069/1‑47 (MQ=255)
                          ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                                               >  1:1359151/1‑47 (MQ=255)
                          ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                                       <  1:5096957/71‑1 (MQ=255)
                           cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                                       >  1:4179180/1‑70 (MQ=255)
                           cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                                       <  1:3390875/70‑1 (MQ=255)
                             gAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaa                                                                    <  1:885608/39‑1 (MQ=255)
                              aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                                          >  1:3888093/1‑64 (MQ=255)
                              aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                                          >  1:1902425/1‑64 (MQ=255)
                                aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                 >  1:5290268/1‑71 (MQ=255)
                                  atatTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag                                         <  1:3366957/61‑1 (MQ=255)
                                    atTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                                       >  1:4202109/1‑61 (MQ=255)
                                     ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc                                               <  1:343597/52‑1 (MQ=255)
                                     ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                             <  1:3432388/70‑1 (MQ=255)
                                     ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                             <  1:639752/70‑1 (MQ=255)
                                      tCCGTTAATCCGGCATTGCGCAGGCCATAAaccacc                                                              >  1:1406758/1‑36 (MQ=255)
                                      tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                             >  1:4215380/1‑69 (MQ=255)
                                       ccGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg                          >  1:3361955/1‑71 (MQ=255)
                                       ccGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGc                             >  1:1502745/1‑68 (MQ=255)
                                        cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga                                        >  1:3009797/1‑56 (MQ=255)
                                        cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                           <  1:2437817/69‑1 (MQ=255)
                                           tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                      >  1:2040723/1‑71 (MQ=255)
                                            aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                      <  1:5190734/70‑1 (MQ=255)
                                              tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                            <  1:4721886/62‑1 (MQ=255)
                                              tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                     <  1:553044/69‑1 (MQ=255)
                                              tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                     <  1:177354/69‑1 (MQ=255)
                                                cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCt                               <  1:4706119/57‑1 (MQ=255)
                                                  gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc                                >  1:1661599/1‑54 (MQ=255)
                                                  gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc                                >  1:5226937/1‑54 (MQ=255)
                                                   cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                >  1:5480005/1‑53 (MQ=255)
                                                   cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                >  1:5413957/1‑53 (MQ=255)
                                                   cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg               >  1:311521/1‑70 (MQ=255)
                                                   cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg               >  1:1359999/1‑70 (MQ=255)
                                                   cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggg                        >  1:1810563/1‑61 (MQ=255)
                                                     ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTgaga                                           <  1:2914534/40‑1 (MQ=255)
                                                     ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTgaga                                           <  1:628327/40‑1 (MQ=255)
                                                     ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTgaga                                           <  1:5646928/40‑1 (MQ=255)
                                                     ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCa             >  1:1753101/1‑70 (MQ=255)
                                                     ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag                                         <  1:5193117/42‑1 (MQ=255)
                                                     ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg            <  1:4337317/71‑1 (MQ=255)
                                                      tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg            >  1:4959400/1‑70 (MQ=255)
                                                      tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                   <  1:1414141/47‑1 (MQ=255)
                                                      tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                   <  1:4538676/47‑1 (MQ=255)
                                                      tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                   <  1:4774993/47‑1 (MQ=255)
                                                      tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                   <  1:5031492/47‑1 (MQ=255)
                                                       gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGAt                                 >  1:4017813/1‑48 (MQ=255)
                                                       gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCAtggc                       >  1:4981360/1‑57 (MQ=255)
                                                       gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGa          >  1:2553886/1‑71 (MQ=255)
                                                       gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                  >  1:1478264/1‑47 (MQ=255)
                                                       gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                  >  1:5657266/1‑47 (MQ=255)
                                                        cgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGAc         <  1:3725958/71‑1 (MQ=255)
                                                          cAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt       >  1:117966/1‑71 (MQ=255)
                                                           aGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACttt      <  1:3163220/71‑1 (MQ=255)
                                                             gCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAc    >  1:3936944/1‑71 (MQ=255)
                                                              ccATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtttt     >  1:176605/1‑68 (MQ=255)
                                                              ccATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtttt     >  1:3117455/1‑68 (MQ=255)
                                                              ccATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                   <  1:5705/39‑1 (MQ=255)
                                                              ccATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                   <  1:4000200/39‑1 (MQ=255)
                                                              ccATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                   <  1:5257019/39‑1 (MQ=255)
                                                               cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                         >  1:1163787/1‑48 (MQ=255)
                                                                aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc         <  1:4301593/63‑1 (MQ=255)
                                                                aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc         <  1:1097056/63‑1 (MQ=255)
                                                                aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc         <  1:192852/63‑1 (MQ=255)
                                                                aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc         <  1:786349/63‑1 (MQ=255)
                                                                aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                             <  1:3889169/43‑1 (MQ=255)
                                                                aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                             <  1:880141/43‑1 (MQ=255)
                                                                aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg                          >  1:5338130/1‑46 (MQ=255)
                                                                 tAAACCACCAGATATATGCCGAGCGAGAAGATGACAATCTGCCAggggg                      <  1:4049902/49‑1 (MQ=255)
                                                                 tAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                   <  1:1484990/36‑1 (MQ=255)
                                                                 tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                             <  1:3462397/42‑1 (MQ=255)
                                                                  aaaCCACCAGATACATGCCGAGCGAGAAGATGACAAt                                 >  1:2089697/1‑37 (MQ=255)
                                                                   aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                           >  1:4664768/1‑42 (MQ=255)
                                                                   aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                           >  1:5545859/1‑42 (MQ=255)
                                                                   aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                           >  1:1519448/1‑42 (MQ=255)
                                                                   aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                  >  1:1532783/1‑51 (MQ=255)
                                                                   |                                                                  
CGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCC  >  Exported/2595652‑2595785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: