Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,728 A→G 30.7% Y290Y (TAT→TAC arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,7280AG30.7% 156.3 / 98.4 127Y290Y (TAT→TACarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (44/44);  new base G (18/21);  total (62/65)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 133 total aligned reads displayed.

CGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCAT  >  Exported/2595659‑2595798
                                                                     |                                                                      
cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                                                        <  1:5487768/70‑1 (MQ=255)
cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                                                        <  1:2578486/70‑1 (MQ=255)
cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                                                        <  1:237230/70‑1 (MQ=255)
cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt                                                                       <  1:4913772/71‑1 (MQ=255)
cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt                                                                       <  1:394464/71‑1 (MQ=255)
    aGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACAt                                                                   >  1:619326/1‑71 (MQ=255)
        cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg                                                               <  1:4507846/71‑1 (MQ=255)
        cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg                                                               <  1:2684351/71‑1 (MQ=255)
                aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa                                                       <  1:3268683/71‑1 (MQ=255)
                  gCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaaaa                                                     <  1:951590/71‑3 (MQ=255)
                   ccAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                                                    <  1:328670/71‑1 (MQ=255)
                   ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                                                    <  1:5096957/71‑1 (MQ=255)
                   ccAGAAAGATATTCCGTTAATCCGGAATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga                                                     >  1:4739359/1‑70 (MQ=255)
                    cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                                                    >  1:4179180/1‑70 (MQ=255)
                    cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                                                    <  1:3390875/70‑1 (MQ=255)
                     aGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGa                                                  <  1:3853428/71‑1 (MQ=255)
                       aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                                                       >  1:1902425/1‑64 (MQ=255)
                       aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                                                       >  1:3888093/1‑64 (MQ=255)
                           atatTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag                                                      <  1:3366957/61‑1 (MQ=255)
                             atTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                                                    >  1:4202109/1‑61 (MQ=255)
                              ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                          <  1:3432388/70‑1 (MQ=255)
                               tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                          >  1:4215380/1‑69 (MQ=255)
                                ccGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg                                       >  1:3361955/1‑71 (MQ=255)
                                ccGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGcc                                                                <  1:1361447/46‑1 (MQ=255)
                                ccGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGc                                          >  1:1502745/1‑68 (MQ=255)
                                 cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga                                                     >  1:3009797/1‑56 (MQ=255)
                                 cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                        <  1:2437817/69‑1 (MQ=255)
                                   ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag                                                         <  1:5134430/50‑1 (MQ=255)
                                    tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                   >  1:2040723/1‑71 (MQ=255)
                                     aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                   <  1:5190734/70‑1 (MQ=255)
                                     aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                   <  1:1520409/70‑1 (MQ=255)
                                       tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                                  <  1:553044/69‑1 (MQ=255)
                                       tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                                  <  1:395201/69‑1 (MQ=255)
                                       tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                                  <  1:177354/69‑1 (MQ=255)
                                         cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCt                                            <  1:4706119/57‑1 (MQ=255)
                                           gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc                                             >  1:5226937/1‑54 (MQ=255)
                                           gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc                                             >  1:1661599/1‑54 (MQ=255)
                                            cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                             >  1:4682640/1‑53 (MQ=255)
                                            cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                             >  1:5413957/1‑53 (MQ=255)
                                            cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                             >  1:5480005/1‑53 (MQ=255)
                                            cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg                            >  1:311521/1‑70 (MQ=255)
                                            cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg                            >  1:1359999/1‑70 (MQ=255)
                                            cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg                            <  1:4448805/70‑1 (MQ=255)
                                            cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggg                                     >  1:1810563/1‑61 (MQ=255)
                                              ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTgaga                                                        <  1:5646928/40‑1 (MQ=255)
                                              ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTgaga                                                        <  1:4334479/40‑1 (MQ=255)
                                              ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTgaga                                                        <  1:628327/40‑1 (MQ=255)
                                              ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCa                          >  1:1753101/1‑70 (MQ=255)
                                              ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg                         <  1:4337317/71‑1 (MQ=255)
                                               tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg                         >  1:4959400/1‑70 (MQ=255)
                                               tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                <  1:4774993/47‑1 (MQ=255)
                                               tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                <  1:4538676/47‑1 (MQ=255)
                                               tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                <  1:1414141/47‑1 (MQ=255)
                                                gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGAt                                              >  1:4017813/1‑48 (MQ=255)
                                                gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                             >  1:5028236/1‑49 (MQ=255)
                                                gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCAtggc                                    >  1:4981360/1‑57 (MQ=255)
                                                gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCAtggc                                    >  1:5300690/1‑57 (MQ=255)
                                                gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAgg                        <  1:1432804/70‑1 (MQ=255)
                                                gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGa                       >  1:2553886/1‑71 (MQ=255)
                                                gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                               >  1:1478264/1‑47 (MQ=255)
                                                gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                               >  1:5657266/1‑47 (MQ=255)
                                                gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc                                             >  1:3537021/1‑49 (MQ=255)
                                                gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc                                             >  1:838677/1‑49 (MQ=255)
                                                gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                       >  1:3144657/1‑71 (MQ=255)
                                                gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                       >  1:5474520/1‑71 (MQ=255)
                                                    aGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACttt                   <  1:3163220/71‑1 (MQ=255)
                                                    aGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt                    <  1:5676939/70‑1 (MQ=255)
                                                     ggCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt                    <  1:2847733/69‑1 (MQ=255)
                                                      gCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAc                 >  1:3936944/1‑71 (MQ=255)
                                                       ccATAAACCACCAGATACATGCCGAGCGAGAAGATGa                                                  >  1:1987143/1‑37 (MQ=255)
                                                       ccATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                <  1:5705/39‑1 (MQ=255)
                                                       ccATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                <  1:5257019/39‑1 (MQ=255)
                                                        cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                                      >  1:1163787/1‑48 (MQ=255)
                                                         aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                      <  1:4301593/63‑1 (MQ=255)
                                                         aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                      <  1:786349/63‑1 (MQ=255)
                                                         aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                      <  1:1097056/63‑1 (MQ=255)
                                                         aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTccgg              <  1:906572/71‑3 (MQ=255)
                                                         aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                          <  1:3889169/43‑1 (MQ=255)
                                                         aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                          <  1:3435103/43‑1 (MQ=255)
                                                         aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg                                       >  1:5338130/1‑46 (MQ=255)
                                                          tAAACCACCAGATATATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                   <  1:4049902/49‑1 (MQ=255)
                                                          tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                          <  1:3462397/42‑1 (MQ=255)
                                                          tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc               <  1:299542/69‑1 (MQ=255)
                                                           aaaCCACCAGATACATGCCGAGCGAGAAGATGACAAt                                              >  1:2089697/1‑37 (MQ=255)
                                                            aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                        >  1:4664768/1‑42 (MQ=255)
                                                            aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                        >  1:5545859/1‑42 (MQ=255)
                                                            aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                        >  1:1519448/1‑42 (MQ=255)
                                                            aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                               >  1:5229832/1‑51 (MQ=255)
                                                             accaccAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAtt          <  1:719264/71‑1 (MQ=255)
                                                              ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc                                          >  1:945133/1‑38 (MQ=255)
                                                              ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc                                          >  1:1025090/1‑38 (MQ=255)
                                                              ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc                                          >  1:1349049/1‑38 (MQ=255)
                                                               caccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa        <  1:257140/71‑1 (MQ=255)
                                                                 ccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATc      <  1:2879642/71‑1 (MQ=255)
                                                                  caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg     <  1:3722434/71‑1 (MQ=255)
                                                                   aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg                         >  1:1036405/1‑50 (MQ=255)
                                                                   aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg     >  1:4186020/1‑70 (MQ=255)
                                                                     aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa        >  1:2385229/1‑65 (MQ=255)
                                                                     aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc    <  1:5510319/69‑1 (MQ=255)
                                                                     aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCAt  <  1:1668657/71‑1 (MQ=255)
                                                                     |                                                                      
CGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCAT  >  Exported/2595659‑2595798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: