Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,740 C→T 35.9% S286S (TCG→TCA arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,7400CT35.9% 128.4 / 115.4 131S286S (TCG→TCAarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base C (44/40);  new base T (23/24);  total (67/64)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

TGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGT  >  Exported/2595670‑2595806
                                                                      |                                                                  
tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc                                                                    >  1:1370406/1‑71 (MQ=255)
     aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa                                                               <  1:3268683/71‑1 (MQ=255)
     aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa                                                               <  1:434414/71‑1 (MQ=255)
      cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag                                                                 >  1:3286343/1‑68 (MQ=255)
       gCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaaaa                                                             <  1:951590/71‑3 (MQ=255)
        ccAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                                                            <  1:328670/71‑1 (MQ=255)
        ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                                                            <  1:5096957/71‑1 (MQ=255)
         cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                                                            <  1:3390875/70‑1 (MQ=255)
         cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                                                            >  1:4179180/1‑70 (MQ=255)
          aGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGa                                                          <  1:3853428/71‑1 (MQ=255)
            aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                                                               >  1:3888093/1‑64 (MQ=255)
            aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                                                               >  1:1902425/1‑64 (MQ=255)
            aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                                                               >  1:5593260/1‑64 (MQ=255)
              aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                      >  1:5290268/1‑71 (MQ=255)
                atatTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag                                                              <  1:3366957/61‑1 (MQ=255)
                  atTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                                                            >  1:4202109/1‑61 (MQ=255)
                   ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc                                                                    <  1:343597/52‑1 (MQ=255)
                   ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                 <  1:5425102/71‑1 (MQ=255)
                   ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                  <  1:639752/70‑1 (MQ=255)
                    tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                  >  1:4215380/1‑69 (MQ=255)
                      cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga                                                             >  1:3009797/1‑56 (MQ=255)
                      cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                                <  1:2437817/69‑1 (MQ=255)
                         tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                           >  1:2040723/1‑71 (MQ=255)
                          aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                           <  1:5190734/70‑1 (MQ=255)
                          aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                           <  1:1520409/70‑1 (MQ=255)
                            tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                 <  1:4721886/62‑1 (MQ=255)
                            tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                                          <  1:177354/69‑1 (MQ=255)
                              cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCt                                                    <  1:4706119/57‑1 (MQ=255)
                                gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc                                                     >  1:1661599/1‑54 (MQ=255)
                                gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc                                                     >  1:5226937/1‑54 (MQ=255)
                                 cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                                     >  1:4682640/1‑53 (MQ=255)
                                 cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                                     >  1:5413957/1‑53 (MQ=255)
                                 cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                                     >  1:5480005/1‑53 (MQ=255)
                                 cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg                                    >  1:1359999/1‑70 (MQ=255)
                                 cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgcg                                    >  1:4283970/1‑70 (MQ=255)
                                 cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgcg                                    >  1:5508891/1‑70 (MQ=255)
                                 cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgcg                                    >  1:3923888/1‑70 (MQ=255)
                                   ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTgaga                                                                <  1:4334479/40‑1 (MQ=255)
                                   ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTgaga                                                                <  1:628327/40‑1 (MQ=255)
                                   ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCa                                  >  1:1753101/1‑70 (MQ=255)
                                   ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag                                                              <  1:5193117/42‑1 (MQ=255)
                                    tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg                                 >  1:4959400/1‑70 (MQ=255)
                                    tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg                                 >  1:2682276/1‑70 (MQ=255)
                                    tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                        <  1:4538676/47‑1 (MQ=255)
                                    tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                        <  1:4774993/47‑1 (MQ=255)
                                     gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGAt                                                      >  1:4017813/1‑48 (MQ=255)
                                     gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                                     >  1:5028236/1‑49 (MQ=255)
                                     gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCAtggc                                            >  1:4981360/1‑57 (MQ=255)
                                     gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCAtggc                                            >  1:5300690/1‑57 (MQ=255)
                                     gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAgg                                <  1:1432804/70‑1 (MQ=255)
                                     gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGa                               >  1:2553886/1‑71 (MQ=255)
                                     gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                                                            >  1:4180699/1‑42 (MQ=255)
                                     gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                                       >  1:5657266/1‑47 (MQ=255)
                                     gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                                       >  1:1478264/1‑47 (MQ=255)
                                     gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc                                                     >  1:3537021/1‑49 (MQ=255)
                                     gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc                                                     >  1:838677/1‑49 (MQ=255)
                                     gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                               >  1:3144657/1‑71 (MQ=255)
                                        cAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt                            >  1:117966/1‑71 (MQ=255)
                                         aGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt                            <  1:5676939/70‑1 (MQ=255)
                                          ggCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt                            <  1:2847733/69‑1 (MQ=255)
                                           gCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAc                         >  1:3936944/1‑71 (MQ=255)
                                            ccATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtttt                          >  1:3117455/1‑68 (MQ=255)
                                            ccATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                        <  1:4000200/39‑1 (MQ=255)
                                            ccATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                        <  1:5257019/39‑1 (MQ=255)
                                             cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                                              >  1:1163787/1‑48 (MQ=255)
                                              aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                              <  1:4301593/63‑1 (MQ=255)
                                              aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                              <  1:786349/63‑1 (MQ=255)
                                              aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                              <  1:192852/63‑1 (MQ=255)
                                              aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                              <  1:3387899/63‑1 (MQ=255)
                                              aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                  <  1:880141/43‑1 (MQ=255)
                                              aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                  <  1:3435103/43‑1 (MQ=255)
                                               tAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                        <  1:1484990/36‑1 (MQ=255)
                                               tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                  <  1:3462397/42‑1 (MQ=255)
                                               tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc                       <  1:299542/69‑1 (MQ=255)
                                                aaaCCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                      >  1:2089697/1‑37 (MQ=255)
                                                 aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                                >  1:5545859/1‑42 (MQ=255)
                                                 aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                                >  1:1519448/1‑42 (MQ=255)
                                                   ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc                                                  >  1:945133/1‑38 (MQ=255)
                                                   ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc                                                  >  1:1025090/1‑38 (MQ=255)
                                                   ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc                                                  >  1:457586/1‑38 (MQ=255)
                                                    caccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                <  1:257140/71‑1 (MQ=255)
                                                      ccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATc              <  1:2879642/71‑1 (MQ=255)
                                                       caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg             <  1:3722434/71‑1 (MQ=255)
                                                        aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg                                 >  1:1036405/1‑50 (MQ=255)
                                                        aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg                                 >  1:234510/1‑50 (MQ=255)
                                                          aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                >  1:2385229/1‑65 (MQ=255)
                                                          aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc            <  1:5510319/69‑1 (MQ=255)
                                                          aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCAt          <  1:1668657/71‑1 (MQ=255)
                                                           tACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAAt          <  1:1845789/70‑1 (MQ=255)
                                                            aCATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGa        >  1:5107652/1‑71 (MQ=255)
                                                             cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGtg                    >  1:5689818/1‑57 (MQ=255)
                                                             cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGtg                    >  1:4378702/1‑57 (MQ=255)
                                                             cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAc       >  1:180481/1‑71 (MQ=255)
                                                             cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAc       >  1:1060550/1‑71 (MQ=255)
                                                               tGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                 <  1:2975736/59‑1 (MQ=255)
                                                               tGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACttt                           <  1:4840439/49‑1 (MQ=255)
                                                                gCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                               >  1:4394685/1‑44 (MQ=255)
                                                                gCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                               >  1:3895040/1‑44 (MQ=255)
                                                                  cgagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt  <  1:4160915/71‑1 (MQ=255)
                                                                    agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt  >  1:1319482/1‑69 (MQ=255)
                                                                      |                                                                  
TGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGT  >  Exported/2595670‑2595806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: