Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,752 A→G 31.4% I282I (ATT→ATC arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,7520AG31.4% 149.8 / 98.9 124I282I (ATT→ATCarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (49/36);  new base G (24/15);  total (73/51)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 134 total aligned reads displayed.

AGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAAT  >  Exported/2595684‑2595821
                                                                    |                                                                     
aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                                     >  1:5290268/1‑71 (MQ=255)
     ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                                 <  1:639752/70‑1 (MQ=255)
     ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                                 <  1:3432388/70‑1 (MQ=255)
       ccGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGc                                                                 >  1:1502745/1‑68 (MQ=255)
        cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                                               <  1:2437817/69‑1 (MQ=255)
           tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                                          >  1:2040723/1‑71 (MQ=255)
            aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                                          <  1:1520409/70‑1 (MQ=255)
            aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                                          <  1:5190734/70‑1 (MQ=255)
              tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                                <  1:4721886/62‑1 (MQ=255)
              tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                                                         <  1:395201/69‑1 (MQ=255)
              tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                                                         <  1:553044/69‑1 (MQ=255)
                   cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                                                    >  1:5413957/1‑53 (MQ=255)
                   cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                                                    >  1:4682640/1‑53 (MQ=255)
                   cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc                                                                    >  1:5480005/1‑53 (MQ=255)
                   cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg                                                   >  1:1359999/1‑70 (MQ=255)
                   cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg                                                   <  1:4448805/70‑1 (MQ=255)
                   cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg                                                   >  1:311521/1‑70 (MQ=255)
                   cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggg                                                            >  1:1810563/1‑61 (MQ=255)
                   cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgcg                                                   >  1:5508891/1‑70 (MQ=255)
                   cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgcg                                                   >  1:4283970/1‑70 (MQ=255)
                   cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgcg                                                   >  1:3923888/1‑70 (MQ=255)
                     ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCa                                                 >  1:1753101/1‑70 (MQ=255)
                      tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg                                                >  1:4959400/1‑70 (MQ=255)
                      tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg                                                >  1:2682276/1‑70 (MQ=255)
                      tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                                       <  1:4774993/47‑1 (MQ=255)
                      tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                                       <  1:5031492/47‑1 (MQ=255)
                      tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                                       <  1:1414141/47‑1 (MQ=255)
                       gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGAt                                                                     >  1:4017813/1‑48 (MQ=255)
                       gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGAt                                                                     >  1:495566/1‑48 (MQ=255)
                       gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCAtggc                                                           >  1:4981360/1‑57 (MQ=255)
                       gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGa                                              >  1:2553886/1‑71 (MQ=255)
                       gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                                                      >  1:5657266/1‑47 (MQ=255)
                       gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                                                      >  1:1478264/1‑47 (MQ=255)
                       gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc                                                                    >  1:3537021/1‑49 (MQ=255)
                       gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                                              >  1:3144657/1‑71 (MQ=255)
                       gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                                              >  1:5474520/1‑71 (MQ=255)
                        cgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGAc                                             <  1:3725958/71‑1 (MQ=255)
                          cAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt                                           >  1:117966/1‑71 (MQ=255)
                           aGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACttt                                          <  1:3163220/71‑1 (MQ=255)
                            ggCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt                                           <  1:2847733/69‑1 (MQ=255)
                             gCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAc                                        >  1:3936944/1‑71 (MQ=255)
                              ccATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtttt                                         >  1:176605/1‑68 (MQ=255)
                              ccATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                                       <  1:5257019/39‑1 (MQ=255)
                              ccATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                                       <  1:5705/39‑1 (MQ=255)
                               cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                                                             >  1:1163787/1‑48 (MQ=255)
                                aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                                             <  1:3387899/63‑1 (MQ=255)
                                aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                                             <  1:786349/63‑1 (MQ=255)
                                aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                                             <  1:4301593/63‑1 (MQ=255)
                                aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                                             <  1:192852/63‑1 (MQ=255)
                                aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTccgg                                     <  1:906572/71‑3 (MQ=255)
                                aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                                 <  1:3435103/43‑1 (MQ=255)
                                aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                                 <  1:3889169/43‑1 (MQ=255)
                                aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                                 <  1:880141/43‑1 (MQ=255)
                                aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg                                                              >  1:5338130/1‑46 (MQ=255)
                                 tAAACCACCAGATATATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                                          <  1:4049902/49‑1 (MQ=255)
                                 tAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                                       <  1:1484990/36‑1 (MQ=255)
                                 tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                                 <  1:3462397/42‑1 (MQ=255)
                                 tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc                                      <  1:299542/69‑1 (MQ=255)
                                  aaaCCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                                     >  1:2089697/1‑37 (MQ=255)
                                   aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                                               >  1:5545859/1‑42 (MQ=255)
                                   aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                                               >  1:4664768/1‑42 (MQ=255)
                                   aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                                                      >  1:1532783/1‑51 (MQ=255)
                                   aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                                                      >  1:5229832/1‑51 (MQ=255)
                                    accaccAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAtt                                 <  1:719264/71‑1 (MQ=255)
                                     ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc                                                                 >  1:457586/1‑38 (MQ=255)
                                     ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc                                                                 >  1:1025090/1‑38 (MQ=255)
                                      caccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                               <  1:257140/71‑1 (MQ=255)
                                        ccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATc                             <  1:2879642/71‑1 (MQ=255)
                                         caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                            <  1:3722434/71‑1 (MQ=255)
                                          aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg                                                >  1:234510/1‑50 (MQ=255)
                                          aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                            >  1:4186020/1‑70 (MQ=255)
                                            aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                               >  1:2385229/1‑65 (MQ=255)
                                            aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCAt                         <  1:1668657/71‑1 (MQ=255)
                                             tACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAAt                         <  1:1845789/70‑1 (MQ=255)
                                              aCATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGa                       >  1:5107652/1‑71 (MQ=255)
                                               cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGtg                                   >  1:4378702/1‑57 (MQ=255)
                                               cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGtg                                   >  1:5689818/1‑57 (MQ=255)
                                               cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAc                      >  1:1060550/1‑71 (MQ=255)
                                               cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAc                      >  1:180481/1‑71 (MQ=255)
                                                 tGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACttt                                          <  1:4840439/49‑1 (MQ=255)
                                                 tGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACttt                                          <  1:4282590/49‑1 (MQ=255)
                                                  gCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                                              >  1:4394685/1‑44 (MQ=255)
                                                    cgagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                 <  1:4160915/71‑1 (MQ=255)
                                                      agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                 >  1:1319482/1‑69 (MQ=255)
                                                          agaaaaTAACGATCTGCCATGGCGCACCGCGCAGGACtt                                           >  1:3309099/1‑39 (MQ=19)
                                                          agaaaaTAACGATCTGCCATGGCGCACCGCGCAGGACTTTTccg                                      >  1:1081720/1‑43 (MQ=255)
                                                           gaagaTGACAATCTTCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                >  1:5332342/1‑65 (MQ=255)
                                                           gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                >  1:2317579/1‑65 (MQ=255)
                                                           gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga          >  1:713254/1‑71 (MQ=255)
                                                            aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc                   >  1:1017289/1‑61 (MQ=255)
                                                               aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgacga       >  1:4151440/1‑70 (MQ=255)
                                                               aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac      <  1:1089310/71‑1 (MQ=255)
                                                               aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac      <  1:4033741/71‑1 (MQ=255)
                                                                tGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa                       >  1:2307392/1‑53 (MQ=255)
                                                                tGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa                       >  1:3044607/1‑53 (MQ=255)
                                                                 gACAATCTGCCAGGTGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGc            >  1:2381533/1‑63 (MQ=255)
                                                                 gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGc            >  1:1600690/1‑63 (MQ=255)
                                                                 gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGc            >  1:2823252/1‑63 (MQ=255)
                                                                 gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa     >  1:1396365/1‑70 (MQ=255)
                                                                  aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa   <  1:1027949/71‑1 (MQ=255)
                                                                    |                                                                     
AGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAAT  >  Exported/2595684‑2595821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: