Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,764 G→C 35.5% A278A (GCC→GCG arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,7640GC35.5% 93.2 / 86.7 93A278A (GCC→GCGarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (33/27);  new base C (17/16);  total (50/43)
Fisher's exact test for biased strand distribution p-value = 8.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

TAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGC  >  Exported/2595695‑2595832
                                                                     |                                                                    
tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                                                     >  1:2040723/1‑71 (MQ=255)
 aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                                                     <  1:5190734/70‑1 (MQ=255)
 aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                                                     <  1:1520409/70‑1 (MQ=255)
   tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                                                                    <  1:177354/69‑1 (MQ=255)
   tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                                                                    <  1:395201/69‑1 (MQ=255)
   tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc                                                                    <  1:553044/69‑1 (MQ=255)
        cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg                                                              >  1:311521/1‑70 (MQ=255)
        cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg                                                              >  1:1359999/1‑70 (MQ=255)
        cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg                                                              <  1:4448805/70‑1 (MQ=255)
        cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgcg                                                              >  1:5508891/1‑70 (MQ=255)
        cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgcg                                                              >  1:3923888/1‑70 (MQ=255)
        cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgcg                                                              >  1:4283970/1‑70 (MQ=255)
          ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCa                                                            >  1:1753101/1‑70 (MQ=255)
          ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg                                                           <  1:4337317/71‑1 (MQ=255)
           tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg                                                           >  1:2682276/1‑70 (MQ=255)
           tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg                                                           >  1:4959400/1‑70 (MQ=255)
            gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAgg                                                          <  1:1432804/70‑1 (MQ=255)
            gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGa                                                         >  1:2553886/1‑71 (MQ=255)
            gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                                                         >  1:3144657/1‑71 (MQ=255)
            gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                                                         >  1:5474520/1‑71 (MQ=255)
             cgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGAc                                                        <  1:3725958/71‑1 (MQ=255)
               cAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt                                                      >  1:117966/1‑71 (MQ=255)
                aGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACttt                                                     <  1:3163220/71‑1 (MQ=255)
                aGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt                                                      <  1:5676939/70‑1 (MQ=255)
                 ggCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt                                                      <  1:2847733/69‑1 (MQ=255)
                  gCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAc                                                   >  1:3936944/1‑71 (MQ=255)
                   ccATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtttt                                                    >  1:3117455/1‑68 (MQ=255)
                   ccATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtttt                                                    >  1:176605/1‑68 (MQ=255)
                     aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                                                        <  1:3387899/63‑1 (MQ=255)
                     aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                                                        <  1:1097056/63‑1 (MQ=255)
                     aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                                                        <  1:4301593/63‑1 (MQ=255)
                     aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                                                        <  1:786349/63‑1 (MQ=255)
                     aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                                                        <  1:192852/63‑1 (MQ=255)
                     aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTccgg                                                <  1:906572/71‑3 (MQ=255)
                      tAAACCACCAGATATATGCCGAGCGAGAAGATGACAATCTGCCAggggg                                                                     <  1:4049902/49‑1 (MQ=255)
                      tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc                                                 <  1:299542/69‑1 (MQ=255)
                        aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                                                                 >  1:5229832/1‑51 (MQ=255)
                        aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                                                                 >  1:1532783/1‑51 (MQ=255)
                         accaccAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAtt                                            <  1:719264/71‑1 (MQ=255)
                           caccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                                          <  1:257140/71‑1 (MQ=255)
                             ccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATc                                        <  1:2879642/71‑1 (MQ=255)
                              caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                                       <  1:3722434/71‑1 (MQ=255)
                               aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg                                                           >  1:234510/1‑50 (MQ=255)
                               aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg                                                           >  1:1036405/1‑50 (MQ=255)
                               aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                                       >  1:4186020/1‑70 (MQ=255)
                                 aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                                          >  1:2385229/1‑65 (MQ=255)
                                 aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc                                      <  1:5510319/69‑1 (MQ=255)
                                 aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCAt                                    <  1:1668657/71‑1 (MQ=255)
                                  tACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAAt                                    <  1:1845789/70‑1 (MQ=255)
                                   aCATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGa                                  >  1:5107652/1‑71 (MQ=255)
                                    cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGtg                                              >  1:5689818/1‑57 (MQ=255)
                                    cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGtg                                              >  1:4378702/1‑57 (MQ=255)
                                    cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAc                                 >  1:180481/1‑71 (MQ=255)
                                    cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAc                                 >  1:1060550/1‑71 (MQ=255)
                                     aTGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc                                                        <  1:2724969/47‑1 (MQ=255)
                                      tGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                                           <  1:2975736/59‑1 (MQ=255)
                                      tGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACttt                                                     <  1:4840439/49‑1 (MQ=255)
                                      tGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACttt                                                     <  1:4282590/49‑1 (MQ=255)
                                       gCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                                                         >  1:3895040/1‑44 (MQ=255)
                                       gCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                                                         >  1:4394685/1‑44 (MQ=255)
                                        ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg                             <  1:2361138/71‑1 (MQ=255)
                                         cgagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                            <  1:4160915/71‑1 (MQ=255)
                                           agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                            >  1:3451039/1‑69 (MQ=255)
                                           agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                            >  1:1319482/1‑69 (MQ=255)
                                               agaaaaTAACGATCTGCCATGGCGCACCGCGCAGGACtt                                                      >  1:3309099/1‑39 (MQ=19)
                                               agaaaaTAACGATCTGCCATGGCGCACCGCGCAGGACTTTTccg                                                 >  1:1081720/1‑43 (MQ=255)
                                                gaagaTGACAATCTTCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                           >  1:5332342/1‑65 (MQ=255)
                                                gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                           >  1:2317579/1‑65 (MQ=255)
                                                gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                           >  1:189617/1‑65 (MQ=255)
                                                gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga                     >  1:713254/1‑71 (MQ=255)
                                                 aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc                              >  1:1017289/1‑61 (MQ=255)
                                                    aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                                           <  1:4941705/45‑1 (MQ=255)
                                                    aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgacga                  >  1:4151440/1‑70 (MQ=255)
                                                    aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                 <  1:4033741/71‑1 (MQ=255)
                                                    aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                 <  1:1089310/71‑1 (MQ=255)
                                                     tGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa                                  >  1:3044607/1‑53 (MQ=255)
                                                     tGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa                                  >  1:2307392/1‑53 (MQ=255)
                                                     tGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa                <  1:694332/71‑1 (MQ=255)
                                                      gACAATCTGCCAGGTGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGc                       >  1:2381533/1‑63 (MQ=255)
                                                      gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGc                       >  1:2823252/1‑63 (MQ=255)
                                                      gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGc                       >  1:1600690/1‑63 (MQ=255)
                                                      gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa                >  1:1396365/1‑70 (MQ=255)
                                                       aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa              <  1:1027949/71‑1 (MQ=255)
                                                       aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa              <  1:4115735/71‑1 (MQ=255)
                                                        cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa              >  1:2115101/1‑70 (MQ=255)
                                                        cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat             <  1:3595832/71‑1 (MQ=255)
                                                          aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                                           <  1:5520304/39‑1 (MQ=255)
                                                          aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                            <  1:3955823/54‑1 (MQ=255)
                                                          aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataa           <  1:5658511/71‑1 (MQ=255)
                                                           tCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaaacag           >  1:1109487/1‑67 (MQ=255)
                                                            cTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa              >  1:3463228/1‑66 (MQ=255)
                                                               ccATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCtttt                          >  1:334792/1‑51 (MQ=255)
                                                               ccATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaaacag           <  1:3102050/66‑4 (MQ=255)
                                                               ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat          <  1:5305533/67‑1 (MQ=255)
                                                               ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat        <  1:5287046/69‑1 (MQ=255)
                                                                cAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAg     >  1:4824907/1‑71 (MQ=255)
                                                                 aGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc                                      >  1:939516/1‑37 (MQ=255)
                                                                   ggggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgcgc  <  1:5058683/71‑1 (MQ=255)
                                                                     |                                                                    
TAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGC  >  Exported/2595695‑2595832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: