Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,788 A→G 8.7% N270N (AAT→AAC arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,7880AG 8.7% 252.5 / 11.1 92N270N (AAT→AACarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (45/39);  new base G (6/2);  total (51/41)
Fisher's exact test for biased strand distribution p-value = 2.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.28e-01

ACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCG  >  Exported/2595720‑2595858
                                                                    |                                                                      
accaccAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAtt                                                                      <  1:719264/71‑1 (MQ=255)
  caccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                                                                    <  1:257140/71‑1 (MQ=255)
    ccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATc                                                                  <  1:2879642/71‑1 (MQ=255)
     caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                                                                 <  1:3722434/71‑1 (MQ=255)
      aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                                                                 >  1:4186020/1‑70 (MQ=255)
        aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                                                                    >  1:2385229/1‑65 (MQ=255)
        aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc                                                                <  1:5510319/69‑1 (MQ=255)
        aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCAt                                                              <  1:1668657/71‑1 (MQ=255)
         tACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAAt                                                              <  1:1845789/70‑1 (MQ=255)
          aCATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGa                                                            >  1:5107652/1‑71 (MQ=255)
           cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAc                                                           >  1:1060550/1‑71 (MQ=255)
           cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAc                                                           >  1:180481/1‑71 (MQ=255)
             tGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                                                                     <  1:2975736/59‑1 (MQ=255)
               ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg                                                       <  1:2361138/71‑1 (MQ=255)
                cgagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                                                      <  1:4160915/71‑1 (MQ=255)
                  agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                                                      >  1:3451039/1‑69 (MQ=255)
                  agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                                                      >  1:1319482/1‑69 (MQ=255)
                       gaagaTGACAATCTTCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                                                     >  1:5332342/1‑65 (MQ=255)
                       gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                                                     >  1:189617/1‑65 (MQ=255)
                       gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                                                     >  1:2317579/1‑65 (MQ=255)
                       gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga                                               >  1:713254/1‑71 (MQ=255)
                        aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc                                                        >  1:1017289/1‑61 (MQ=255)
                           aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                                                                     <  1:4941705/45‑1 (MQ=255)
                           aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgacga                                            >  1:4151440/1‑70 (MQ=255)
                           aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                                           <  1:4033741/71‑1 (MQ=255)
                           aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                                           <  1:1089310/71‑1 (MQ=255)
                            tGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa                                                            >  1:2307392/1‑53 (MQ=255)
                            tGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa                                                            >  1:3044607/1‑53 (MQ=255)
                            tGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa                                          <  1:694332/71‑1 (MQ=255)
                             gACAATCTGCCAGGTGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGc                                                 >  1:2381533/1‑63 (MQ=255)
                             gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGc                                                 >  1:2823252/1‑63 (MQ=255)
                             gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGc                                                 >  1:1600690/1‑63 (MQ=255)
                             gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa                                          >  1:1396365/1‑70 (MQ=255)
                              aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                                        <  1:4115735/71‑1 (MQ=255)
                              aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                                        <  1:1027949/71‑1 (MQ=255)
                               cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                                        >  1:2115101/1‑70 (MQ=255)
                               cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat                                       <  1:3595832/71‑1 (MQ=255)
                                 aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                                                                     <  1:5520304/39‑1 (MQ=255)
                                 aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                                                      <  1:3955823/54‑1 (MQ=255)
                                 aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataa                                     <  1:5658511/71‑1 (MQ=255)
                                  tCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaaacag                                     >  1:1109487/1‑67 (MQ=255)
                                   cTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                                        >  1:3463228/1‑66 (MQ=255)
                                      ccATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCtttt                                                    >  1:334792/1‑51 (MQ=255)
                                      ccATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaaacag                                     <  1:3102050/66‑4 (MQ=255)
                                      ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat                                    <  1:5305533/67‑1 (MQ=255)
                                      ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat                                  <  1:5287046/69‑1 (MQ=255)
                                       cAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAg                               >  1:4824907/1‑71 (MQ=255)
                                        aGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc                                                                >  1:939516/1‑37 (MQ=255)
                                          ggggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgcgc                            <  1:5058683/71‑1 (MQ=255)
                                              cACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGcc                         <  1:3093418/70‑1 (MQ=255)
                                               aCCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaaa                                        >  1:4772379/1‑54 (MQ=255)
                                                ccGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCca                       >  1:317747/1‑70 (MQ=255)
                                                 cgcgCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCcaca                     <  1:3079868/71‑1 (MQ=255)
                                                 cgcgCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCca                       >  1:3932763/1‑69 (MQ=255)
                                                  gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataa                                     >  1:3531616/1‑54 (MQ=255)
                                                  gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgcg                             >  1:4576056/1‑62 (MQ=255)
                                                  gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAg                    >  1:2575954/1‑71 (MQ=255)
                                                  gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAg                    >  1:3221159/1‑71 (MQ=255)
                                                  gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAg                    >  1:4191834/1‑71 (MQ=255)
                                                  gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAg                    >  1:533624/1‑71 (MQ=255)
                                                  gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAg                    >  1:4817201/1‑71 (MQ=255)
                                                   cgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGc                   >  1:3481100/1‑71 (MQ=255)
                                                   cgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGc                   >  1:2619880/1‑71 (MQ=255)
                                                    gcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGc                   >  1:1332667/1‑70 (MQ=255)
                                                    gcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGc                   >  1:4719319/1‑70 (MQ=255)
                                                    gcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCt                  <  1:649150/71‑1 (MQ=255)
                                                     cAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTg                 >  1:4706815/1‑71 (MQ=255)
                                                     cAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTg                 >  1:4868977/1‑71 (MQ=255)
                                                     cAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTg                 >  1:2949618/1‑71 (MQ=255)
                                                       ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                                              <  1:2011494/40‑1 (MQ=255)
                                                       ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                                              <  1:5274742/40‑1 (MQ=255)
                                                       ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                                              <  1:1290140/40‑1 (MQ=255)
                                                       ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat                                       <  1:484439/47‑1 (MQ=255)
                                                       ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGc                >  1:3915310/1‑70 (MQ=255)
                                                        gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa              <  1:1299457/71‑1 (MQ=255)
                                                        gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa              <  1:548972/71‑1 (MQ=255)
                                                         aCTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAataata                                   >  1:3168700/1‑49 (MQ=255)
                                                         aCTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAataata                                   >  1:5628449/1‑49 (MQ=255)
                                                            ttACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAg                    <  1:2908000/61‑1 (MQ=255)
                                                             tACCCGTATTAATCGCATGACCGCGTTTAGcgacga                                            >  1:4852765/1‑36 (MQ=255)
                                                             tACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATg            >  1:1924365/1‑68 (MQ=255)
                                                              aCCCGTATTAATCGCATGACCGCGTTTAGCGACGACa                                          <  1:5552354/37‑1 (MQ=255)
                                                              aCCCGTATTAATCGCATGACCGCGTTTAGCGACGACa                                          <  1:2451467/37‑1 (MQ=255)
                                                              aCCCGTATTAATCGCATGACCGCGTTTAGCGACGACa                                          <  1:3747462/37‑1 (MQ=255)
                                                              aCCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCt                  >  1:2392865/1‑61 (MQ=255)
                                                              aCCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCt                  >  1:3693062/1‑61 (MQ=255)
                                                                ccGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTc      <  1:4287425/71‑1 (MQ=255)
                                                                   tATTAATCGCATGACCGCGTTTAGCGACGACAAataata                                   <  1:1426728/39‑1 (MQ=255)
                                                                   tATTAATCGCATGACCGCGTTTAGCGACGACAAataata                                   <  1:1089890/39‑1 (MQ=255)
                                                                   tATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat                                  <  1:2900300/40‑1 (MQ=255)
                                                                   tATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCca                       >  1:462970/1‑51 (MQ=255)
                                                                   tATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCca                       >  1:1029097/1‑51 (MQ=255)
                                                                    aTTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCg  >  1:1817278/1‑71 (MQ=255)
                                                                    |                                                                      
ACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCG  >  Exported/2595720‑2595858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: