Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,791 A→G 8.5% I269I (ATT→ATC arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,7910AG 8.5% 261.9 / 11.1 94I269I (ATT→ATCarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (48/38);  new base G (6/2);  total (54/40)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCGG  >  Exported/2595722‑2595859
                                                                     |                                                                    
caccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                                                                     <  1:257140/71‑1 (MQ=255)
  ccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATc                                                                   <  1:2879642/71‑1 (MQ=255)
   caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                                                                  <  1:3722434/71‑1 (MQ=255)
    aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                                                                  >  1:4186020/1‑70 (MQ=255)
      aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                                                                     >  1:2385229/1‑65 (MQ=255)
      aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc                                                                 <  1:5510319/69‑1 (MQ=255)
      aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCAt                                                               <  1:1668657/71‑1 (MQ=255)
       tACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAAt                                                               <  1:1845789/70‑1 (MQ=255)
        aCATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGa                                                             >  1:5107652/1‑71 (MQ=255)
         cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAc                                                            >  1:1060550/1‑71 (MQ=255)
         cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAc                                                            >  1:180481/1‑71 (MQ=255)
           tGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                                                                      <  1:2975736/59‑1 (MQ=255)
             ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg                                                        <  1:2361138/71‑1 (MQ=255)
              cgagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                                                       <  1:4160915/71‑1 (MQ=255)
                agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                                                       >  1:3451039/1‑69 (MQ=255)
                agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                                                       >  1:1319482/1‑69 (MQ=255)
                     gaagaTGACAATCTTCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                                                      >  1:5332342/1‑65 (MQ=255)
                     gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                                                      >  1:189617/1‑65 (MQ=255)
                     gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt                                                      >  1:2317579/1‑65 (MQ=255)
                     gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga                                                >  1:713254/1‑71 (MQ=255)
                      aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc                                                         >  1:1017289/1‑61 (MQ=255)
                         aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                                                                      <  1:4941705/45‑1 (MQ=255)
                         aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgacga                                             >  1:4151440/1‑70 (MQ=255)
                         aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                                            <  1:4033741/71‑1 (MQ=255)
                         aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac                                            <  1:1089310/71‑1 (MQ=255)
                          tGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa                                                             >  1:2307392/1‑53 (MQ=255)
                          tGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa                                                             >  1:3044607/1‑53 (MQ=255)
                          tGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa                                           <  1:694332/71‑1 (MQ=255)
                           gACAATCTGCCAGGTGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGc                                                  >  1:2381533/1‑63 (MQ=255)
                           gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGc                                                  >  1:2823252/1‑63 (MQ=255)
                           gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGc                                                  >  1:1600690/1‑63 (MQ=255)
                           gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa                                           >  1:1396365/1‑70 (MQ=255)
                            aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                                         <  1:4115735/71‑1 (MQ=255)
                            aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                                         <  1:1027949/71‑1 (MQ=255)
                             cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                                         >  1:2115101/1‑70 (MQ=255)
                             cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat                                        <  1:3595832/71‑1 (MQ=255)
                               aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                                                                      <  1:5520304/39‑1 (MQ=255)
                               aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt                                                       <  1:3955823/54‑1 (MQ=255)
                               aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataa                                      <  1:5658511/71‑1 (MQ=255)
                                tCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaaacag                                      >  1:1109487/1‑67 (MQ=255)
                                 cTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa                                         >  1:3463228/1‑66 (MQ=255)
                                    ccATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCtttt                                                     >  1:334792/1‑51 (MQ=255)
                                    ccATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaaacag                                      <  1:3102050/66‑4 (MQ=255)
                                    ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat                                     <  1:5305533/67‑1 (MQ=255)
                                    ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat                                   <  1:5287046/69‑1 (MQ=255)
                                     cAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAg                                >  1:4824907/1‑71 (MQ=255)
                                      aGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc                                                                 >  1:939516/1‑37 (MQ=255)
                                        ggggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgcgc                             <  1:5058683/71‑1 (MQ=255)
                                            cACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGcc                          <  1:3093418/70‑1 (MQ=255)
                                             aCCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaaa                                         >  1:4772379/1‑54 (MQ=255)
                                              ccGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCca                        >  1:317747/1‑70 (MQ=255)
                                               cgcgCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCcaca                      <  1:3079868/71‑1 (MQ=255)
                                               cgcgCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCca                        >  1:3932763/1‑69 (MQ=255)
                                                gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataa                                      >  1:3531616/1‑54 (MQ=255)
                                                gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgcg                              >  1:4576056/1‑62 (MQ=255)
                                                gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAg                     >  1:533624/1‑71 (MQ=255)
                                                gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAg                     >  1:2575954/1‑71 (MQ=255)
                                                gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAg                     >  1:4191834/1‑71 (MQ=255)
                                                gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAg                     >  1:4817201/1‑71 (MQ=255)
                                                gcgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAg                     >  1:3221159/1‑71 (MQ=255)
                                                 cgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGc                    >  1:3481100/1‑71 (MQ=255)
                                                 cgcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGc                    >  1:2619880/1‑71 (MQ=255)
                                                  gcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGc                    >  1:4719319/1‑70 (MQ=255)
                                                  gcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGc                    >  1:1332667/1‑70 (MQ=255)
                                                  gcAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCt                   <  1:649150/71‑1 (MQ=255)
                                                   cAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTg                  >  1:2949618/1‑71 (MQ=255)
                                                   cAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTg                  >  1:4868977/1‑71 (MQ=255)
                                                   cAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTg                  >  1:4706815/1‑71 (MQ=255)
                                                     ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                                               <  1:2011494/40‑1 (MQ=255)
                                                     ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                                               <  1:1290140/40‑1 (MQ=255)
                                                     ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgac                                               <  1:5274742/40‑1 (MQ=255)
                                                     ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat                                        <  1:484439/47‑1 (MQ=255)
                                                     ggACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGc                 >  1:3915310/1‑70 (MQ=255)
                                                      gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa               <  1:548972/71‑1 (MQ=255)
                                                      gACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa               <  1:1299457/71‑1 (MQ=255)
                                                       aCTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAataata                                    >  1:3168700/1‑49 (MQ=255)
                                                       aCTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAataata                                    >  1:5628449/1‑49 (MQ=255)
                                                          ttACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAg                     <  1:2908000/61‑1 (MQ=255)
                                                           tACCCGTATTAATCGCATGACCGCGTTTAGcgacga                                             >  1:4852765/1‑36 (MQ=255)
                                                           tACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATg             >  1:1924365/1‑68 (MQ=255)
                                                            aCCCGTATTAATCGCATGACCGCGTTTAGCGACGACa                                           <  1:3747462/37‑1 (MQ=255)
                                                            aCCCGTATTAATCGCATGACCGCGTTTAGCGACGACa                                           <  1:5552354/37‑1 (MQ=255)
                                                            aCCCGTATTAATCGCATGACCGCGTTTAGCGACGACa                                           <  1:2451467/37‑1 (MQ=255)
                                                            aCCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCt                   >  1:2392865/1‑61 (MQ=255)
                                                            aCCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCt                   >  1:3693062/1‑61 (MQ=255)
                                                              ccGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTc       <  1:4287425/71‑1 (MQ=255)
                                                                 tATTAATCGCATGACCGCGTTTAGCGACGACAAataata                                    <  1:1426728/39‑1 (MQ=255)
                                                                 tATTAATCGCATGACCGCGTTTAGCGACGACAAataata                                    <  1:1089890/39‑1 (MQ=255)
                                                                 tATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat                                   <  1:2900300/40‑1 (MQ=255)
                                                                 tATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCca                        >  1:1029097/1‑51 (MQ=255)
                                                                 tATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCca                        >  1:462970/1‑51 (MQ=255)
                                                                  aTTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCg   >  1:1817278/1‑71 (MQ=255)
                                                                   ttAATCGCATGACCGCGTTTAGCGACGACAaataat                                     <  1:2693475/36‑1 (MQ=255)
                                                                   ttAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa               >  1:2064077/1‑58 (MQ=255)
                                                                   ttAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCaa               >  1:2240880/1‑58 (MQ=255)
                                                                   ttAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCgg  <  1:554643/71‑1 (MQ=255)
                                                                   ttAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCgg  <  1:2520740/71‑1 (MQ=255)
                                                                   ttAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCg   >  1:4282271/1‑70 (MQ=255)
                                                                    tAATCGCATGACCGCGTTTAGCGACGACAAataata                                    <  1:2746013/36‑1 (MQ=255)
                                                                     |                                                                    
CACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGCGCCCACAGCTGCAATGGCGCTCACCGG  >  Exported/2595722‑2595859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: