Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,596,088	G→C	10.4%	L170L (CTC→CTG)	arsB ←	arsenite/antimonite transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,596,088	0	G	C	10.4%	238.5 / 14.2	86	L170L (CTC→CTG)	arsB	arsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (30/47);  new base C (6/3);  total (36/50)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

CATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTA  >  Exported/2596019‑2596154                                                                       |                                                                   cATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGa                                                                   <  1:95430/71‑1 (MQ=255)  aTCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGa                                                                   <  1:2708317/70‑1 (MQ=255)     aCCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGc                                                                >  1:1023456/1‑70 (MQ=255)     aCCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGc                                                                >  1:900574/1‑70 (MQ=255)     aCCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGc                                                                >  1:3730694/1‑70 (MQ=255)     aCCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGc                                                                >  1:1413762/1‑70 (MQ=255)         gCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCaa                                                             <  1:1658216/69‑1 (MQ=255)              gCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATc                                                      <  1:4271830/71‑1 (MQ=255)                 acaaTCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGc                                                   >  1:4656980/1‑71 (MQ=255)                    aTCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGa                                                <  1:350982/71‑1 (MQ=255)                       gcggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGaaa                                              <  1:4626850/70‑1 (MQ=255)                       gcggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGaaa                                              <  1:4620175/70‑1 (MQ=255)                         ggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGa                                           <  1:1529366/71‑1 (MQ=255)                           cgATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         >  1:5208722/1‑71 (MQ=255)                              tatCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGa                                                <  1:2569275/61‑1 (MQ=255)                               atCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCa                                     >  1:3750295/1‑71 (MQ=255)                                tCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         >  1:4246305/1‑66 (MQ=255)                                tCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         >  1:4212338/1‑66 (MQ=255)                                tCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         >  1:3468187/1‑66 (MQ=255)                                tCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTc                                      >  1:2542745/1‑69 (MQ=255)                                  cACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGaa                                                         <  1:1592809/48‑1 (MQ=255)                                  cACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAAt                                                       >  1:3275166/1‑50 (MQ=255)                                  cACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAcc                                   <  1:4238865/70‑1 (MQ=255)                                  cACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCa                                  <  1:2734764/71‑1 (MQ=255)                                   aCCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGaaa                                                        >  1:35870/1‑48 (MQ=255)                                    ccGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGaa                                                         <  1:5404506/46‑1 (MQ=255)                                    ccGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAAt                                                       >  1:1408805/1‑48 (MQ=255)                                    ccGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGa                                                <  1:245251/55‑1 (MQ=255)                                     cGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGt                               <  1:3055166/71‑1 (MQ=255)                                      ggCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGaaa                                              >  1:878055/1‑55 (MQ=255)                                      ggCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt                              <  1:2563728/71‑1 (MQ=255)                                       gCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                             <  1:3655638/71‑1 (MQ=255)                                        gatcATAACGGAGGCGTACT‑GCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGt                               >  1:1748718/4‑68 (MQ=255)                                          ccATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATc                                                      <  1:3048243/43‑1 (MQ=255)                                          ccATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATc                                                      <  1:2693380/43‑1 (MQ=255)                                          ccATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATc                                                      <  1:1598296/43‑1 (MQ=255)                                          ccATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATc                                                      <  1:642444/43‑1 (MQ=255)                                          ccATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                             <  1:3571826/52‑1 (MQ=255)                                           cATAACGGAGGCGTACT‑GCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaaa                         >  1:3207204/1‑69 (MQ=255)                                           cATAACGGAGGCGTACT‑GCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaaa                         >  1:2358490/1‑69 (MQ=255)                                           cATAACGGAGGCGTACT‑GCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaa                          >  1:4646796/1‑69 (MQ=255)                                             tCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                             <  1:2312640/49‑1 (MQ=255)                                               aCCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                             >  1:467677/1‑63 (MQ=255)                                               aCCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACaa                      >  1:2641538/1‑70 (MQ=255)                                               aCCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                     <  1:1330378/71‑1 (MQ=255)                                               aCCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                     <  1:249723/71‑1 (MQ=255)                                                 cGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAg                                                    <  1:2248939/38‑1 (MQ=255)                                                 cGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGa                                           <  1:1179556/47‑1 (MQ=255)                                                 cGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                     >  1:3443319/1‑69 (MQ=255)                                                     gCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCgg               >  1:1004157/1‑71 (MQ=255)                                                      cGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGc              <  1:4355585/71‑1 (MQ=255)                                                        tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                             <  1:2006210/38‑1 (MQ=255)                                                        tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACg                                            <  1:3665311/39‑1 (MQ=255)                                                        tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACg                                            <  1:5605021/39‑1 (MQ=255)                                                        tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACg                                            <  1:3508099/39‑1 (MQ=255)                                                        tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAg                                 <  1:1461946/50‑1 (MQ=255)                                                        tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAg                                 <  1:3006230/50‑1 (MQ=255)                                                        tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGACATTCACCAg                                 <  1:1082108/50‑1 (MQ=255)                                                         aTTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAgg                                >  1:349147/1‑50 (MQ=255)                                                         aTTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                    <  1:1196447/62‑1 (MQ=255)                                                          ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                             <  1:2061554/36‑1 (MQ=255)                                                          ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAc                                    <  1:223291/45‑1 (MQ=255)                                                          ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                             >  1:4434754/1‑52 (MQ=255)                                                          ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                             >  1:2173524/1‑52 (MQ=255)                                                          ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGAAATTCACAAGGTTGGAGACAATAAGCGGCAgg           >  1:2493236/1‑70 (MQ=255)                                                           tCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                             <  1:3576795/35‑1 (MQ=255)                                                           tCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         >  1:2091012/1‑39 (MQ=255)                                                           tCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAg                  >  1:5291843/1‑62 (MQ=255)                                                            cgcg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAc                                    >  1:5292188/1‑43 (MQ=255)                                                             gcg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         <  1:1122080/37‑1 (MQ=255)                                                              cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg       >  1:3393996/4‑71 (MQ=255)                                                              cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg       >  1:3387305/4‑71 (MQ=255)                                                              cg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc      >  1:1196194/1‑71 (MQ=255)                                                              cg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc      <  1:1877612/71‑1 (MQ=255)                                                              cg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc      <  1:3513256/71‑1 (MQ=255)                                                              cg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc      >  1:351590/1‑71 (MQ=255)                                                               g‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt                              <  1:519162/46‑1 (MQ=255)                                                               g‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt                              <  1:4460923/46‑1 (MQ=255)                                                               g‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCg     <  1:3270281/71‑1 (MQ=255)                                                                caaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACa                       <  1:5531407/52‑1 (MQ=255)                                                                 aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg               <  1:11626/60‑1 (MQ=255)                                                                 aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg               <  1:3444343/60‑1 (MQ=255)                                                                 aaaGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTTGAGACAATAAGCGGCAGGCTGGCgg    >  1:2759706/1‑71 (MQ=255)                                                                   aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCa                                  <  1:5092252/39‑1 (MQ=255)                                                                   aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTa  >  1:4325507/1‑71 (MQ=255)                                                                    gCCGAGGCCAAATAAATCAGCGGAAACGATATTCACCAGGtt                              <  1:4819783/42‑1 (MQ=255)                                                                       |                                                                   CATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTA  >  Exported/2596019‑2596154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑