Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,596,103 A→C 15.2% A165A (GCT→GCG arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,596,1030AC15.2% 196.7 / 37.1 99A165A (GCT→GCGarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (37/47);  new base C (7/8);  total (44/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

ACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGG  >  Exported/2596035‑2596170
                                                                     |                                                                   
acaaTCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGc                                                                   >  1:4656980/1‑71 (MQ=255)
   aTCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGa                                                                <  1:350982/71‑1 (MQ=255)
      gcggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGaaa                                                              <  1:4620175/70‑1 (MQ=255)
      gcggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGaaa                                                              <  1:4626850/70‑1 (MQ=255)
        ggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGa                                                           <  1:1529366/71‑1 (MQ=255)
          cgATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                                         >  1:5208722/1‑71 (MQ=255)
             tatCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGa                                                                <  1:2569275/61‑1 (MQ=255)
              atCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCa                                                     >  1:3750295/1‑71 (MQ=255)
               tCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                                         >  1:4212338/1‑66 (MQ=255)
               tCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                                         >  1:4246305/1‑66 (MQ=255)
               tCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                                         >  1:3468187/1‑66 (MQ=255)
               tCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTc                                                      >  1:2542745/1‑69 (MQ=255)
                 cACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAcc                                                   <  1:4238865/70‑1 (MQ=255)
                 cACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCa                                                  <  1:2734764/71‑1 (MQ=255)
                   ccGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGa                                                                <  1:245251/55‑1 (MQ=255)
                    cGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGt                                               <  1:3055166/71‑1 (MQ=255)
                     ggCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGaaa                                                              >  1:878055/1‑55 (MQ=255)
                     ggCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt                                              <  1:2563728/71‑1 (MQ=255)
                      gCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                                             <  1:3655638/71‑1 (MQ=255)
                       gatcATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGt                                               >  1:1748718/4‑68 (MQ=255)
                         ccATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                                             <  1:3571826/52‑1 (MQ=255)
                          cATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaaa                                         >  1:3207204/1‑69 (MQ=255)
                          cATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaaa                                         >  1:2358490/1‑69 (MQ=255)
                          cATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaa                                          >  1:4646796/1‑69 (MQ=255)
                            tCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                                             <  1:2312640/49‑1 (MQ=255)
                              aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                                             >  1:467677/1‑63 (MQ=255)
                              aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACaa                                      >  1:2641538/1‑70 (MQ=255)
                              aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                                     <  1:249723/71‑1 (MQ=255)
                              aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                                     <  1:1330378/71‑1 (MQ=255)
                                cGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAg                                                                    <  1:2248939/38‑1 (MQ=255)
                                cGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGa                                                           <  1:1179556/47‑1 (MQ=255)
                                cGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                                     >  1:3443319/1‑69 (MQ=255)
                                    gCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCgg                               >  1:1004157/1‑71 (MQ=255)
                                     cGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGc                              <  1:4355585/71‑1 (MQ=255)
                                       tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                                             <  1:2006210/38‑1 (MQ=255)
                                       tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACg                                                            <  1:3508099/39‑1 (MQ=255)
                                       tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACg                                                            <  1:5605021/39‑1 (MQ=255)
                                       tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACg                                                            <  1:3665311/39‑1 (MQ=255)
                                       tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAg                                                 <  1:1461946/50‑1 (MQ=255)
                                       tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAg                                                 <  1:3006230/50‑1 (MQ=255)
                                       tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGACATTCACCAg                                                 <  1:1082108/50‑1 (MQ=255)
                                        aTTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAgg                                                >  1:349147/1‑50 (MQ=255)
                                        aTTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                                    <  1:1196447/62‑1 (MQ=255)
                                         ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                                             <  1:2061554/36‑1 (MQ=255)
                                         ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAc                                                    <  1:223291/45‑1 (MQ=255)
                                         ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                                             >  1:2173524/1‑52 (MQ=255)
                                         ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                                             >  1:4434754/1‑52 (MQ=255)
                                         ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGAAATTCACAAGGTTGGAGACAATAAGCGGCAgg                           >  1:2493236/1‑70 (MQ=255)
                                          tCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                                             <  1:3576795/35‑1 (MQ=255)
                                          tCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                                         >  1:2091012/1‑39 (MQ=255)
                                          tCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAg                                  >  1:5291843/1‑62 (MQ=255)
                                           cgcgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAc                                                    >  1:5292188/1‑43 (MQ=255)
                                            gcgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                                         <  1:1122080/37‑1 (MQ=255)
                                             cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg                       >  1:3387305/4‑71 (MQ=255)
                                             cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg                       >  1:3393996/4‑71 (MQ=255)
                                             cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc                      >  1:351590/1‑71 (MQ=255)
                                             cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc                      <  1:3513256/71‑1 (MQ=255)
                                             cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc                      >  1:1196194/1‑71 (MQ=255)
                                             cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc                      <  1:1877612/71‑1 (MQ=255)
                                              gAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt                                              <  1:4460923/46‑1 (MQ=255)
                                              gAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt                                              <  1:519162/46‑1 (MQ=255)
                                              gAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCg                     <  1:3270281/71‑1 (MQ=255)
                                               caaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACa                                       <  1:5531407/52‑1 (MQ=255)
                                                aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg                               <  1:11626/60‑1 (MQ=255)
                                                aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg                               <  1:3444343/60‑1 (MQ=255)
                                                aaaGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTTGAGACAATAAGCGGCAGGCTGGCgg                    >  1:2759706/1‑71 (MQ=255)
                                                  aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCa                                                  <  1:5092252/39‑1 (MQ=255)
                                                  aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTa                  >  1:4325507/1‑71 (MQ=255)
                                                   gCCGAGGCCAAATAAATCAGCGGAAACGATATTCACCAGGtt                                              <  1:4819783/42‑1 (MQ=255)
                                                        ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga                                           >  1:5066925/1‑40 (MQ=255)
                                                        ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga                                           >  1:3288686/1‑40 (MQ=255)
                                                        ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga                                           >  1:4005825/1‑40 (MQ=255)
                                                        ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga                                           >  1:3708164/1‑40 (MQ=255)
                                                        ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga                                           >  1:4091978/1‑40 (MQ=255)
                                                        ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga                                           >  1:1260618/1‑40 (MQ=255)
                                                         gCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                                             <  1:15690/37‑1 (MQ=255)
                                                         gCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                                    <  1:303257/46‑1 (MQ=255)
                                                          ccgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCaa           <  1:4561973/67‑1 (MQ=255)
                                                          ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                                    <  1:5329182/45‑1 (MQ=255)
                                                          ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt          <  1:61551/71‑1 (MQ=255)
                                                           cgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAta         <  1:1027594/69‑2 (MQ=255)
                                                           cgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAt          >  1:568668/3‑70 (MQ=255)
                                                            gaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAtaa        <  1:1074104/70‑3 (MQ=255)
                                                            gaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAtaa        <  1:2907473/70‑3 (MQ=255)
                                                            aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCa            <  1:254872/67‑1 (MQ=255)
                                                            aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGa        <  1:2147117/71‑1 (MQ=255)
                                                             aagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGc             <  1:2102362/65‑1 (MQ=255)
                                                             aaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGaa       <  1:1532620/71‑1 (MQ=255)
                                                              aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCgg                               <  1:2897764/46‑1 (MQ=255)
                                                              aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAAt      >  1:343780/1‑71 (MQ=255)
                                                              aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAAt      >  1:2210402/1‑71 (MQ=255)
                                                               gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                                    >  1:4757351/1‑40 (MQ=255)
                                                               gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                                    >  1:627901/1‑40 (MQ=255)
                                                               gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTgg                       >  1:5434049/1‑53 (MQ=255)
                                                               gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc     <  1:1474192/71‑1 (MQ=255)
                                                               gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc     <  1:1231880/71‑1 (MQ=255)
                                                               gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc     >  1:8113/1‑71 (MQ=255)
                                                                  aTCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCgg  >  1:25208/1‑71 (MQ=255)
                                                                    cAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCgg                    >  1:3068564/1‑51 (MQ=255)
                                                                     |                                                                   
ACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGG  >  Exported/2596035‑2596170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: