Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,596,109 A→G 15.9% V163V (GTT→GTC arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,596,1090AG15.9% 211.0 / 29.6 94V163V (GTT→GTCarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (35/44);  new base G (7/8);  total (42/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

GCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAT  >  Exported/2596041‑2596177
                                                                     |                                                                    
gcggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGaaa                                                                     <  1:4626850/70‑1 (MQ=255)
gcggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGaaa                                                                     <  1:4620175/70‑1 (MQ=255)
  ggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGa                                                                  <  1:1529366/71‑1 (MQ=255)
    cgATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                                                >  1:5208722/1‑71 (MQ=255)
        atCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCa                                                            >  1:3750295/1‑71 (MQ=255)
         tCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                                                >  1:4246305/1‑66 (MQ=255)
         tCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                                                >  1:3468187/1‑66 (MQ=255)
         tCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                                                >  1:4212338/1‑66 (MQ=255)
         tCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTc                                                             >  1:2542745/1‑69 (MQ=255)
           cACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAcc                                                          <  1:4238865/70‑1 (MQ=255)
           cACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCa                                                         <  1:2734764/71‑1 (MQ=255)
              cGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGt                                                      <  1:3055166/71‑1 (MQ=255)
               ggCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGaaa                                                                     >  1:878055/1‑55 (MQ=255)
               ggCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt                                                     <  1:2563728/71‑1 (MQ=255)
                gCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                                                    <  1:3655638/71‑1 (MQ=255)
                 gatcATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGt                                                      >  1:1748718/4‑68 (MQ=255)
                   ccATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                                                    <  1:3571826/52‑1 (MQ=255)
                    cATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaaa                                                >  1:2358490/1‑69 (MQ=255)
                    cATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaaa                                                >  1:3207204/1‑69 (MQ=255)
                    cATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaa                                                 >  1:4646796/1‑69 (MQ=255)
                      tCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                                                    <  1:2312640/49‑1 (MQ=255)
                        aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                                                    >  1:467677/1‑63 (MQ=255)
                        aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACaa                                             >  1:2641538/1‑70 (MQ=255)
                        aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                                            <  1:1330378/71‑1 (MQ=255)
                        aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                                            <  1:249723/71‑1 (MQ=255)
                          cGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGa                                                                  <  1:1179556/47‑1 (MQ=255)
                          cGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                                            >  1:3443319/1‑69 (MQ=255)
                              gCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCgg                                      >  1:1004157/1‑71 (MQ=255)
                               cGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGc                                     <  1:4355585/71‑1 (MQ=255)
                                 tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                                                    <  1:2006210/38‑1 (MQ=255)
                                 tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACg                                                                   <  1:3665311/39‑1 (MQ=255)
                                 tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACg                                                                   <  1:3508099/39‑1 (MQ=255)
                                 tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACg                                                                   <  1:5605021/39‑1 (MQ=255)
                                 tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAg                                                        <  1:1461946/50‑1 (MQ=255)
                                 tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAg                                                        <  1:3006230/50‑1 (MQ=255)
                                 tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGACATTCACCAg                                                        <  1:1082108/50‑1 (MQ=255)
                                  aTTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAgg                                                       >  1:349147/1‑50 (MQ=255)
                                  aTTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                                           <  1:1196447/62‑1 (MQ=255)
                                   ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                                                    <  1:2061554/36‑1 (MQ=255)
                                   ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAc                                                           <  1:223291/45‑1 (MQ=255)
                                   ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                                                    >  1:2173524/1‑52 (MQ=255)
                                   ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                                                    >  1:4434754/1‑52 (MQ=255)
                                   ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGAAATTCACAAGGTTGGAGACAATAAGCGGCAgg                                  >  1:2493236/1‑70 (MQ=255)
                                    tCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                                                    <  1:3576795/35‑1 (MQ=255)
                                    tCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                                                >  1:2091012/1‑39 (MQ=255)
                                    tCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAg                                         >  1:5291843/1‑62 (MQ=255)
                                     cgcgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAc                                                           >  1:5292188/1‑43 (MQ=255)
                                      gcgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                                                <  1:1122080/37‑1 (MQ=255)
                                       cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg                              >  1:3387305/4‑71 (MQ=255)
                                       cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg                              >  1:3393996/4‑71 (MQ=255)
                                       cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc                             <  1:1877612/71‑1 (MQ=255)
                                       cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc                             >  1:1196194/1‑71 (MQ=255)
                                       cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc                             <  1:3513256/71‑1 (MQ=255)
                                       cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc                             >  1:351590/1‑71 (MQ=255)
                                        gAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt                                                     <  1:4460923/46‑1 (MQ=255)
                                        gAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt                                                     <  1:519162/46‑1 (MQ=255)
                                        gAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCg                            <  1:3270281/71‑1 (MQ=255)
                                         caaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACa                                              <  1:5531407/52‑1 (MQ=255)
                                          aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg                                      <  1:11626/60‑1 (MQ=255)
                                          aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg                                      <  1:3444343/60‑1 (MQ=255)
                                          aaaGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTTGAGACAATAAGCGGCAGGCTGGCgg                           >  1:2759706/1‑71 (MQ=255)
                                            aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCa                                                         <  1:5092252/39‑1 (MQ=255)
                                            aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTa                         >  1:4325507/1‑71 (MQ=255)
                                             gCCGAGGCCAAATAAATCAGCGGAAACGATATTCACCAGGtt                                                     <  1:4819783/42‑1 (MQ=255)
                                                  ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga                                                  >  1:1260618/1‑40 (MQ=255)
                                                  ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga                                                  >  1:3708164/1‑40 (MQ=255)
                                                  ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga                                                  >  1:4005825/1‑40 (MQ=255)
                                                  ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga                                                  >  1:4091978/1‑40 (MQ=255)
                                                  ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga                                                  >  1:3288686/1‑40 (MQ=255)
                                                  ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga                                                  >  1:5066925/1‑40 (MQ=255)
                                                   gCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                                                    <  1:15690/37‑1 (MQ=255)
                                                   gCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                                           <  1:303257/46‑1 (MQ=255)
                                                    ccgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCaa                  <  1:4561973/67‑1 (MQ=255)
                                                    ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                                           <  1:5329182/45‑1 (MQ=255)
                                                    ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt                 <  1:61551/71‑1 (MQ=255)
                                                     cgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAta                <  1:1027594/69‑2 (MQ=255)
                                                     cgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAt                 >  1:568668/3‑70 (MQ=255)
                                                      gaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAtaa               <  1:1074104/70‑3 (MQ=255)
                                                      gaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAtaa               <  1:2907473/70‑3 (MQ=255)
                                                      aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCa                   <  1:254872/67‑1 (MQ=255)
                                                      aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGa               <  1:2147117/71‑1 (MQ=255)
                                                       aagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGc                    <  1:2102362/65‑1 (MQ=255)
                                                       aaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGaa              <  1:1532620/71‑1 (MQ=255)
                                                        aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCgg                                      <  1:2897764/46‑1 (MQ=255)
                                                        aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAAt             >  1:343780/1‑71 (MQ=255)
                                                        aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAAt             >  1:2210402/1‑71 (MQ=255)
                                                         gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                                           >  1:4757351/1‑40 (MQ=255)
                                                         gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                                           >  1:627901/1‑40 (MQ=255)
                                                         gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTgg                              >  1:5434049/1‑53 (MQ=255)
                                                         gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc            <  1:1474192/71‑1 (MQ=255)
                                                         gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc            <  1:1231880/71‑1 (MQ=255)
                                                         gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc            >  1:8113/1‑71 (MQ=255)
                                                            aTCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCgg         >  1:25208/1‑71 (MQ=255)
                                                              cAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCgg                           >  1:3068564/1‑51 (MQ=255)
                                                                 aggAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGc                    <  1:920563/54‑1 (MQ=255)
                                                                   gAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt  <  1:5361754/71‑1 (MQ=255)
                                                                   gAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt  <  1:332899/71‑1 (MQ=255)
                                                                     |                                                                    
GCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAT  >  Exported/2596041‑2596177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: