BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,677,390	T→C	10.8%	Q180Q (CAA→CAG)	yhgA ←	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,677,390	0	T	C	10.8%	270.8 / 18.4	102	Q180Q (CAA→CAG)	yhgA	predicted transposase
Reads supporting (aligned to +/- strand): ref base T (47/44); new base C (3/8); total (50/52)
Fisher's exact test for biased strand distribution p-value = 2.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 110 total aligned reads displayed.

TATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATC  >  Exported/2677322‑2677458

tATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGttttt                                                                     <  1:5301903/70‑1 (MQ=255)

tATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTg                                                                    <  1:430274/71‑1 (MQ=255)

tATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTg                                                                    <  1:3757877/71‑1 (MQ=255)

  tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa                                                                  >  1:5289948/1‑71 (MQ=255)

  tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa                                                                  <  1:4807672/71‑1 (MQ=255)

  tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa                                                                  <  1:2669028/71‑1 (MQ=255)

  tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGa                                                                   <  1:770257/70‑1 (MQ=255)

  tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGa                                                                   <  1:3618521/70‑1 (MQ=255)

   ccACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGa                                                                   <  1:3673723/69‑1 (MQ=255)

    cACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAt                                                                 >  1:2094361/1‑70 (MQ=255)

         ctTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAAc                                                             <  1:4661692/69‑1 (MQ=255)

         ctTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACt                                                            >  1:2388732/1‑70 (MQ=255)

         ctTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTc                                                           <  1:165960/71‑1 (MQ=255)

            aataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcag                                                        >  1:383153/1‑71 (MQ=255)

            aataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTAca                                                         >  1:5037432/1‑70 (MQ=255)

             ataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt                                                                <  1:4338020/62‑1 (MQ=255)

             ataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt                                                                <  1:4677594/62‑1 (MQ=255)

             ataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcag                                                        <  1:1463852/70‑1 (MQ=255)

                aGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGa                                                                   >  1:1966492/1‑56 (MQ=255)

                aGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTaa                                                              >  1:5587430/1‑61 (MQ=255)

                aGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCagcagc                                                    >  1:947605/1‑71 (MQ=255)

                 gCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAgcg                                                   <  1:4180252/71‑1 (MQ=255)

                   aGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAgcgc                                                  <  1:1133053/70‑1 (MQ=255)

                    ggCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTcc                                                          <  1:4007194/61‑1 (MQ=255)

                     gCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa                                                >  1:652662/1‑70 (MQ=255)

                       cATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAc                                             <  1:4373978/71‑1 (MQ=255)

                         tttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGttttt                                                                     <  1:3022136/45‑1 (MQ=255)

                         tttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGtttt                                                                      <  1:140259/44‑1 (MQ=255)

                         tttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTg                                                                    <  1:629521/46‑1 (MQ=255)

                           tGCTCTGCCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATa                                              >  1:728882/1‑66 (MQ=255)

                           tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGa                                                                   >  1:5650178/1‑45 (MQ=255)

                           tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGa                                                                   >  1:3003453/1‑45 (MQ=255)

                           tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGa                                                                   >  1:1475368/1‑45 (MQ=255)

                           tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt                                                                >  1:1889876/1‑48 (MQ=255)

                           tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt                                                                >  1:1226108/1‑48 (MQ=255)

                           tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTaa                                                              >  1:4003943/1‑50 (MQ=255)

                           tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCca                                                         >  1:2951029/1‑55 (MQ=255)

                           tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                         >  1:4488858/1‑71 (MQ=255)

                           tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                         >  1:1580988/1‑71 (MQ=255)

                           tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                         >  1:5485432/1‑71 (MQ=255)

                           tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                         >  1:1750307/1‑71 (MQ=255)

                           tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGAGCCATACGTCg                                         >  1:4893237/1‑71 (MQ=255)

                             ctctACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCgg                                        <  1:2703192/70‑1 (MQ=255)

                              tctACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtg                                      >  1:140882/1‑71 (MQ=255)

                                tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTcc                                                          <  1:2680006/49‑1 (MQ=255)

                                tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTcc                                                          <  1:2938500/49‑1 (MQ=255)

                                  ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGa                                                                   <  1:3916674/38‑1 (MQ=255)

                                  ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGa                                                                   <  1:2000484/38‑1 (MQ=255)

                                  ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg                                   >  1:2235050/1‑70 (MQ=255)

                                  ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg                                   >  1:15778/1‑70 (MQ=255)

                                  ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg                                   >  1:2848944/1‑70 (MQ=255)

                                  ccAGCCCCATCAGGTCGCGTAGACGAATATGTTTTTGAATTAACTc                                                           >  1:4835249/1‑46 (MQ=255)

                                   cAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa                                                <  1:5516490/56‑1 (MQ=255)

                                    aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtg                                      >  1:4615940/1‑65 (MQ=255)

                                    aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtg                                      >  1:2862998/1‑65 (MQ=255)

                                     atcccAACAGATCGCGCTGACGAATATGTTTCTGAATTaa                                                              >  1:2150796/3‑40 (MQ=16)

                                     atcccAACAGATCGCGCTGACGAATATGTTTCTGAATTaa                                                              >  1:231706/3‑40 (MQ=16)

                                     atcccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAt                                <  1:2602926/68‑1 (MQ=255)

                                      tcccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAtaa                              >  1:5350984/2‑69 (MQ=255)

                                        ccATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAtt                            <  1:1087263/71‑1 (MQ=255)

                                        ccATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAtt                            <  1:4987377/71‑1 (MQ=255)

                                           tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCca                                                         <  1:2903329/39‑1 (MQ=255)

                                           tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCca                                                         <  1:1901199/39‑1 (MQ=255)

                                           tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAc                                             <  1:55238/51‑1 (MQ=255)

                                           tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCa                                 >  1:4551637/1‑63 (MQ=255)

                                           tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtca                         >  1:2979993/1‑71 (MQ=255)

                                            cAGGTCGCGTTGACGAATATGTTTTTGAATTAACTc                                                           >  1:901676/1‑36 (MQ=255)

                                            cAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtca                         >  1:4320673/1‑70 (MQ=255)

                                             aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                        >  1:1993096/1‑70 (MQ=255)

                                             aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                        >  1:3514677/1‑70 (MQ=255)

                                             aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                        >  1:3072811/1‑70 (MQ=255)

                                               gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                           <  1:4713278/49‑1 (MQ=255)

                                               gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                     <  1:2660088/71‑1 (MQ=255)

                                                  gcgcTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCtcgtcatcc                   <  1:1036944/70‑2 (MQ=255)

                                                  gcgcTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCtcgtcatcc                   <  1:4977092/70‑2 (MQ=255)

                                                  gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCgg                                        >  1:5460968/1‑49 (MQ=255)

                                                   cgctGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCgg                 <  1:5353350/68‑1 (MQ=255)

                                                     ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                                          <  1:886975/44‑1 (MQ=255)

                                                     ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa               <  1:5202700/71‑1 (MQ=255)

                                                      tGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt              >  1:3925169/1‑71 (MQ=255)

                                                       gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt              >  1:1599291/1‑70 (MQ=255)

                                                        aCGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt              <  1:921756/69‑1 (MQ=255)

                                                          gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACg          >  1:2702693/1‑71 (MQ=255)

                                                          gAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAta                               <  1:3992153/50‑2 (MQ=255)

                                                          gAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAta                               <  1:3023090/50‑2 (MQ=255)

                                                          gAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAta                               <  1:5350784/50‑2 (MQ=255)

                                                            atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt        <  1:2240814/71‑1 (MQ=255)

                                                            atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt        >  1:5192344/1‑71 (MQ=255)

                                                            atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt        >  1:1891867/1‑71 (MQ=255)

                                                            atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt        >  1:665995/1‑71 (MQ=255)

                                                            atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt        >  1:1906093/1‑71 (MQ=255)

                                                             tatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGa                              >  1:1334354/1‑48 (MQ=255)

                                                             tatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt        <  1:889489/70‑1 (MQ=255)

                                                               tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGAc     <  1:389629/71‑1 (MQ=255)

                                                               tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGAc     <  1:1823691/71‑1 (MQ=255)

                                                                gTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACa    >  1:1055009/1‑71 (MQ=255)

                                                                 tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAg                  <  1:5547609/56‑1 (MQ=255)

                                                                  ttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACat   >  1:2246251/1‑70 (MQ=255)

                                                                  ttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACat   >  1:2630057/1‑70 (MQ=255)

                                                                   tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatc  <  1:3539959/70‑1 (MQ=255)

TATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATC  >  Exported/2677322‑2677458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`