Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 132,196 T→C 8.8% D163D (GAT→GAC yadD → predicted transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported132,1960TC 8.8% 215.1 / 9.5 79D163D (GAT→GACyadDpredicted transposase
Reads supporting (aligned to +/- strand):  ref base T (33/39);  new base C (5/2);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

CTCGCCGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACT  >  Exported/132127‑132266
                                                                     |                                                                      
cTCGCCGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGAt                                                                        <  1:4283319/70‑1 (MQ=255)
 gcGCCGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGAt                                                                        <  1:2152463/68‑1 (MQ=255)
     cGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGaa                                                                  <  1:3473239/71‑1 (MQ=255)
       gAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGAt                                                                        <  1:1125649/63‑1 (MQ=255)
                cgACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAAc                                                        <  1:3049631/70‑1 (MQ=255)
                    gcgTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACg                                                                    <  1:5691943/54‑1 (MQ=255)
                    gcgTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACg                                                                    <  1:5569931/54‑1 (MQ=255)
                    gcgTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACg                                                                    <  1:1316281/54‑1 (MQ=255)
                      gTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATcggcg                                                 <  1:3638659/71‑1 (MQ=255)
                       tCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCTTGCAACATcggcgg                                                <  1:3914110/71‑1 (MQ=255)
                        cTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGaaa                                                                 <  1:1689273/53‑1 (MQ=255)
                         tataACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGAt                                                                        >  1:1353671/1‑45 (MQ=255)
                         tataACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGAt                                                                        >  1:2145813/1‑45 (MQ=255)
                         tataACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGAt                                                                        >  1:2034776/1‑45 (MQ=255)
                         tataACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGAt                                                                        >  1:4939152/1‑45 (MQ=255)
                         tataACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGAt                                              >  1:4600577/1‑71 (MQ=255)
                          ataACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGAtt                                             >  1:4143045/1‑71 (MQ=255)
                             aCAGTCCTTTCCCGCTGGTGGATATCACCATCACCCCGGATGACGAAATCATGCAACATcg                                                    >  1:2266476/1‑61 (MQ=255)
                             aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCaa                                                         >  1:3525250/1‑56 (MQ=255)
                             aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATcg                                                    >  1:488148/1‑61 (MQ=255)
                             aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATcg                                                    >  1:541766/1‑61 (MQ=255)
                             aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGc                                           <  1:3522708/70‑1 (MQ=255)
                             aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGc                                           <  1:2161053/70‑1 (MQ=255)
                              cAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa                                         <  1:1986915/71‑1 (MQ=255)
                                gTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGa                                                                   >  1:5162473/1‑43 (MQ=255)
                                gTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGa                                                                   >  1:1980737/1‑43 (MQ=255)
                                   cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                    <  1:1800412/71‑1 (MQ=255)
                                    tttCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                    >  1:648428/1‑70 (MQ=255)
                                     ttCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCTACATCGGCGGATTGCGATTCTCg                                   >  1:2903375/1‑70 (MQ=255)
                                      tCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                 >  1:5084483/1‑71 (MQ=255)
                                       cccGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                   <  1:4883306/68‑1 (MQ=255)
                                        ccGCTGGTGGATATCACCATCACACCGGATGACGAAAt                                                                <  1:4520174/38‑1 (MQ=255)
                                        ccGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                <  1:4287679/70‑1 (MQ=255)
                                         cGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                <  1:3269773/69‑1 (MQ=255)
                                           cTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAAc                                                        >  1:3978847/1‑43 (MQ=255)
                                           cTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                              >  1:5320433/1‑69 (MQ=255)
                                           cTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                              >  1:1291540/1‑69 (MQ=255)
                                              gtggATATCACCATCACACCGGATGACGAAATCATGCAACATcggcg                                                 <  1:152848/47‑1 (MQ=255)
                                              gtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCa                         <  1:5028866/71‑1 (MQ=255)
                                                ggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                       <  1:879856/71‑1 (MQ=255)
                                                 gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                      <  1:3186170/71‑1 (MQ=255)
                                                 gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                      <  1:2104096/71‑1 (MQ=255)
                                                 gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                       <  1:5527922/70‑1 (MQ=255)
                                                  atatCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTAtag                                            <  1:3532925/48‑3 (MQ=255)
                                                  atatCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                      <  1:2659004/70‑1 (MQ=255)
                                                     taaCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                              >  1:3366443/3‑59 (MQ=255)
                                                     tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGct                                          >  1:1639548/1‑46 (MQ=255)
                                                     tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                   >  1:4331714/1‑70 (MQ=255)
                                                     tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                              >  1:1715133/1‑59 (MQ=255)
                                                     tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                              >  1:5194850/1‑59 (MQ=255)
                                                     tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                              >  1:3654363/1‑59 (MQ=255)
                                                     tCACAATCACACCGGATGACGAAATCATGCAACATc                                                     >  1:1642589/1‑36 (MQ=255)
                                                      cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                 >  1:4456591/1‑71 (MQ=255)
                                                      cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                  >  1:3151006/1‑70 (MQ=255)
                                                      cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                  >  1:5139991/1‑70 (MQ=255)
                                                      cACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                 >  1:137935/1‑71 (MQ=255)
                                                       aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                 <  1:1682564/70‑1 (MQ=255)
                                                       aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                <  1:5411175/71‑1 (MQ=255)
                                                          aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCg             <  1:4109234/71‑1 (MQ=255)
                                                           tCACAGCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                   <  1:793773/48‑1 (MQ=255)
                                                           tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                            >  1:2371299/1‑39 (MQ=255)
                                                           tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                            >  1:1589802/1‑39 (MQ=255)
                                                           tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                            >  1:3622995/1‑39 (MQ=255)
                                                           tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                            >  1:2733784/1‑39 (MQ=255)
                                                           tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                            >  1:3342140/1‑39 (MQ=255)
                                                           tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                              <  1:1364574/53‑1 (MQ=255)
                                                           tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc            <  1:3733406/71‑1 (MQ=255)
                                                           tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc            <  1:1990572/71‑1 (MQ=255)
                                                           tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc            <  1:2811198/71‑1 (MQ=255)
                                                           tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc            >  1:1724271/1‑71 (MQ=255)
                                                           tCACACCGGATGACGAAATCATGCAACATCGGAGGATTGCGATTCTCg                                   <  1:3171776/48‑1 (MQ=255)
                                                            cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                 >  1:283599/1‑49 (MQ=255)
                                                            cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                 >  1:1119891/1‑49 (MQ=255)
                                                            cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                 >  1:1469273/1‑49 (MQ=255)
                                                            cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                 >  1:2427586/1‑49 (MQ=255)
                                                            cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGa                                  >  1:824333/1‑48 (MQ=255)
                                                             acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc           <  1:5225852/70‑1 (MQ=255)
                                                             acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc           <  1:1456262/70‑1 (MQ=255)
                                                               acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                      <  1:4922131/57‑1 (MQ=255)
                                                               acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                      <  1:1591358/57‑1 (MQ=255)
                                                                 cGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc      <  1:1982600/71‑1 (MQ=255)
                                                                 cGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc      >  1:5603944/1‑71 (MQ=255)
                                                                 cGGATGACGAAATCATGCAACATAGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcg       >  1:4314735/1‑70 (MQ=255)
                                                                  ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                   <  1:4411448/57‑1 (MQ=255)
                                                                  ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                   <  1:5499548/57‑1 (MQ=255)
                                                                    aTGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaat                      <  1:1765041/52‑2 (MQ=255)
                                                                     tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                <  1:3950972/41‑1 (MQ=255)
                                                                     tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                <  1:1419189/41‑1 (MQ=255)
                                                                     tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                <  1:2405323/41‑1 (MQ=255)
                                                                     tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                <  1:5469016/41‑1 (MQ=255)
                                                                     tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc            <  1:5480886/61‑1 (MQ=255)
                                                                     tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACt  <  1:5105414/71‑1 (MQ=255)
                                                                     |                                                                      
CTCGCCGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACT  >  Exported/132127‑132266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: