| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,773,711 | T→G | 9.3% | intergenic (+100/‑83) | aceA → / → aceK | isocitrate lyase/isocitrate dehydrogenase kinase/phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,773,711 | 0 | T | G | 9.3% | 142.6 / 7.7 | 54 | intergenic (+100/‑83) | aceA/aceK | isocitrate lyase/isocitrate dehydrogenase kinase/phosphatase |
| Reads supporting (aligned to +/- strand): ref base T (34/15); new base G (3/2); total (37/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
ATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCTGA > Exported/2773643‑2773781 | aTAAGGCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGCTTGCGCGTCTTATCAGGcc > 1:3814546/1‑71 (MQ=11) aaGGCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGCTTGCGCGTCTTATCAGGCCTa > 1:234268/1‑71 (MQ=11) aGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCt < 1:3737784/70‑1 (MQ=255) ggCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGCTTGCGCGTCTTATCAGGCCTa > 1:3284257/1‑69 (MQ=11) ggCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGCTTGCGCGTCTTATCAGGCCTACa < 1:169435/71‑1 (MQ=11) ggCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGCTTGCGCGTCTTATCAGGCCTACa < 1:3713134/71‑1 (MQ=11) ggCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTAc < 1:423264/71‑1 (MQ=255) ttCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGcc > 1:4729389/1‑71 (MQ=255) ttCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGcc < 1:5093041/71‑1 (MQ=255) aCGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGtt > 1:4200124/1‑71 (MQ=255) aCGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGtt > 1:27977/1‑71 (MQ=255) cGGCAATCGGTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGt < 1:2852359/70‑1 (MQ=255) gCAATCGGTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAg < 1:1052830/70‑1 (MQ=255) cGGTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa > 1:423878/1‑71 (MQ=255) cGGTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa > 1:2656109/1‑71 (MQ=255) cGGTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa > 1:1168418/1‑71 (MQ=255) ggTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa > 1:1673876/1‑70 (MQ=255) ggTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt < 1:1384998/71‑1 (MQ=255) ggTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt < 1:604840/71‑1 (MQ=255) ggTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt < 1:2272111/71‑1 (MQ=255) tGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt > 1:5650249/1‑69 (MQ=255) gCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt > 1:1162796/1‑68 (MQ=255) gCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt > 1:2142608/1‑68 (MQ=255) gCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt > 1:4762110/1‑68 (MQ=255) cACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa > 1:5216082/1‑66 (MQ=255) cACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa > 1:4325146/1‑66 (MQ=255) cACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa > 1:4695927/1‑66 (MQ=255) cACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATa > 1:2144495/1‑68 (MQ=255) cACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTAAAGCCGTTGCCGAACGTAGGCTGGa > 1:3206706/1‑66 (MQ=255) aCGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGc < 1:272331/71‑1 (MQ=255) cGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCg > 1:2472219/1‑71 (MQ=255) gA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGt < 1:5626522/71‑1 (MQ=255) gA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGt > 1:2886272/1‑71 (MQ=255) a‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCg < 1:2427619/49‑1 (MQ=255) a‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGtt < 1:1874956/71‑1 (MQ=255) a‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGtt < 1:322042/71‑1 (MQ=255) gCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTa > 1:2939369/1‑71 (MQ=255) aCGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATcc < 1:3811722/71‑1 (MQ=255) tGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCaa > 1:828734/1‑71 (MQ=255) tGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCaa > 1:362289/1‑71 (MQ=255) gcgcGTCTTATCATGCCTACAGCCGTTGCCGAACGTAgg > 1:4326455/1‑39 (MQ=255) gcgcGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAAt > 1:5665366/1‑71 (MQ=255) gcgTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAAtta > 1:5341484/1‑70 (MQ=255) gcgTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATtc > 1:5077689/1‑71 (MQ=255) gTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATtct > 1:2856052/1‑70 (MQ=255) gTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATtct > 1:612470/1‑70 (MQ=255) gTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATtct > 1:1405066/1‑70 (MQ=255) cTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTg > 1:4105112/1‑71 (MQ=255) ttATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGc > 1:3984998/1‑71 (MQ=255) tATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCt > 1:3960715/1‑71 (MQ=255) aTCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTc > 1:3329012/1‑71 (MQ=255) aTGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATtctc > 1:3557179/1‑63 (MQ=255) aTGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATtctc > 1:2383481/1‑63 (MQ=255) aTGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCtg < 1:4509052/71‑1 (MQ=255) tGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCtga > 1:149999/1‑71 (MQ=255) | ATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAATTCTCTGCTCCTGA > Exported/2773643‑2773781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |