| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,815,684 | C→T | 8.7% | S447L (TCG→TTG) ‡ | yjcC → | predicted signal transduction protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,815,684 | 0 | C | T | 8.7% | 360.2 / 16.5 | 126 | S447L (TCG→TTG) ‡ | yjcC | predicted signal transduction protein |
| Reads supporting (aligned to +/- strand): ref base C (63/52); new base T (6/5); total (69/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01 | |||||||||||
Only 100 of 128 total aligned reads displayed.
TTTGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACATCATCGAGCTGGCGCA > Exported/2815614‑2815753 | tGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGt > 1:2413972/1‑71 (MQ=255) ggTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGt < 1:1090777/70‑1 (MQ=255) gTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGtt < 1:3759331/70‑1 (MQ=255) tATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTttg < 1:2032062/71‑1 (MQ=255) tATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTttg < 1:2320603/71‑1 (MQ=255) tATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTttg < 1:2781343/71‑1 (MQ=255) ttGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTttgtt > 1:1088338/1‑71 (MQ=255) ttGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTttgtt > 1:4072580/1‑71 (MQ=255) cTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGa < 1:1529827/69‑1 (MQ=255) ctctAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGc > 1:3956435/1‑71 (MQ=255) tctAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGtt < 1:5502758/59‑1 (MQ=255) tctAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGtt < 1:2245627/59‑1 (MQ=255) tctAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGt < 1:479358/58‑1 (MQ=255) tctAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCt > 1:3400956/1‑71 (MQ=255) tAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGttt > 1:1420465/1‑58 (MQ=255) tAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGttt > 1:1427127/1‑58 (MQ=255) tAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTttgtt > 1:460033/1‑61 (MQ=255) tAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTttgtt > 1:592447/1‑61 (MQ=255) tAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGaaa > 1:4222603/1‑65 (MQ=255) tAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGa > 1:4399615/1‑71 (MQ=255) tAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGa > 1:3938950/1‑71 (MQ=255) tAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGa > 1:912302/1‑71 (MQ=255) tAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGa > 1:1709405/1‑71 (MQ=255) aaCTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGac > 1:463367/1‑71 (MQ=255) aCTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGac > 1:483272/1‑70 (MQ=255) aCTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTCTTGAAACGCTGac < 1:850357/70‑1 (MQ=255) ttGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGaaa > 1:561390/1‑61 (MQ=255) ttGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGaccac > 1:5203008/1‑71 (MQ=255) gCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGaccacc > 1:3727663/1‑70 (MQ=255) gCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGaccacc > 1:2993040/1‑70 (MQ=255) cATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGt < 1:3265703/49‑1 (MQ=255) cATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGt < 1:4477376/49‑1 (MQ=255) cATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGt < 1:2548269/49‑1 (MQ=255) aTAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTttgttg < 1:5201628/54‑1 (MQ=255) aTAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTttgttg < 1:3578873/54‑1 (MQ=255) aTAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTttgttg < 1:5418769/54‑1 (MQ=255) aTAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTttgttg < 1:4874957/54‑1 (MQ=255) aTAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGAccacccac < 1:498839/71‑1 (MQ=255) tAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTttgttg > 1:4663855/1‑53 (MQ=255) tAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGAccacccac > 1:3312843/1‑70 (MQ=255) aCCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACaa < 1:2115828/70‑1 (MQ=255) tatgaaatcgttgccaatcgacgtacTGAAAATCGACAAAATGTTTGTTGa > 1:4187642‑M2/27‑51 (MQ=255) ccTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGa > 1:2026041/1‑59 (MQ=255) ccTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACaaaa > 1:4903493/1‑71 (MQ=255) ttAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGAccacccac > 1:5573808/1‑65 (MQ=255) tgaaatcgttgccaatcgacgtacTGAAAATCGACAAAATGTTTGTTGa > 1:4385557‑M2/25‑49 (MQ=255) tAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTttgtt < 1:2192643/46‑1 (MQ=255) tAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTttgtt < 1:886091/46‑1 (MQ=255) gaaatcgttgccaatcgacgtacTGAAAATCGACAAAATGTttgt < 1:2889127‑M2/22‑1 (MQ=255) gaaatcgttgccaatcgacgtacTGAAAATCGACAAAATGTttg > 1:2922799‑M2/24‑44 (MQ=255) aaatcgttgccaatcgacgtacTGAAAATCGACAAAATGTTTGTTgaag < 1:4058801‑M2/27‑2 (MQ=255) aaatcgttgccaatcgacgtacTGAAAATCGACAAAATGTTTGTTgaag < 1:2269489‑M2/27‑2 (MQ=255) aaatcgttgccaatcgacgtacTGAAAATCGACAAAATGTTTGTTGaa > 1:3871146‑M2/23‑48 (MQ=255) aTCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGa < 1:1185098/45‑1 (MQ=255) aTTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGacca > 1:3370698/1‑53 (MQ=255) aTTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCAtt > 1:1173682/1‑71 (MQ=255) aTTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCAtt < 1:4760136/71‑1 (MQ=255) ttGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCAttt < 1:4652944/71‑1 (MQ=255) tGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCAttt > 1:4613681/1‑70 (MQ=255) tGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCAttt > 1:4608613/1‑70 (MQ=255) gAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTg < 1:5608972/70‑1 (MQ=255) gAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTg < 1:694757/70‑1 (MQ=255) gAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTg < 1:4652426/70‑1 (MQ=255) gAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTg < 1:5046863/70‑1 (MQ=255) caatcgacgtacTGAAAATCGACAAAATGTTTGTTGaa < 1:1671011‑M2/26‑1 (MQ=255) tGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACaaaa > 1:2593308/1‑56 (MQ=255) tGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACaaaa > 1:868876/1‑56 (MQ=255) tCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCAttt < 1:3680171/64‑1 (MQ=255) tCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGc < 1:5340932/70‑1 (MQ=255) cGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCg > 1:1347737/1‑70 (MQ=255) atatTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACaa < 1:1327556/49‑1 (MQ=255) tttGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAAcaca > 1:392349/1‑71 (MQ=255) ttGAAACTCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCTGAACAcat > 1:735192/1‑71 (MQ=255) ttGAAAATCGACAAATCGTTTGTTGAAACGCTGAccaccc > 1:3046562/1‑40 (MQ=255) ttGAAAATCGACAAATCGTTTGTTGAAACGCTGAccaccc > 1:4885020/1‑40 (MQ=255) ttGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGaa > 1:4962514/1‑66 (MQ=255) ttGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACAcat > 1:1166616/1‑71 (MQ=255) ttGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACAcat > 1:2151033/1‑71 (MQ=255) ttGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACAcat > 1:1005062/1‑71 (MQ=255) ttGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCAACAAAACCAGTCATTTGa > 1:4773964/1‑58 (MQ=255) gAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGa > 1:4116012/1‑56 (MQ=255) gAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACAcat < 1:4035606/69‑1 (MQ=255) aTCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACATCATCGa < 1:296538/71‑1 (MQ=255) tCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCAttt < 1:1133706/49‑1 (MQ=255) tCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCAttt < 1:5252856/49‑1 (MQ=255) cGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACATCATCGa > 1:101863/1‑69 (MQ=255) cGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACATCATCGa > 1:1455899/1‑69 (MQ=255) cGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTAGa > 1:1865632/1‑50 (MQ=255) gACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACATCATCGATCt < 1:4833386/71‑1 (MQ=255) aCAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGaa > 1:283417/1‑56 (MQ=255) aaaTCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACATCATCGAGCTgg > 1:4384444/1‑70 (MQ=255) aaTCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACATCATCGAGCt < 1:272342/67‑1 (MQ=255) aTCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTc < 1:1466316/38‑1 (MQ=255) aTCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTc < 1:4174442/38‑1 (MQ=255) tCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGaa > 1:3233850/1‑51 (MQ=255) tCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAac > 1:256685/1‑52 (MQ=255) tCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACATCATCGAGCTGgcgc < 1:2228258/70‑1 (MQ=255) tCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACATCATCGAGCTGGCGca > 1:1810201/1‑71 (MQ=255) tCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGAgg > 1:5526892/1‑49 (MQ=255) cGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACATCATCGAGCTGGCGca > 1:1272135/1‑70 (MQ=255) | TTTGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACATCATCGAGCTGGCGCA > Exported/2815614‑2815753 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |