| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 312,582 | A→C | 5.0% | G97G (GGT→GGG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 312,582 | 0 | A | C | 5.0% | 335.5 / 8.7 | 119 | G97G (GGT→GGG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (51/62); new base C (4/2); total (55/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
TTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGT > Exported/312513‑312652 | ttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:5200020/1‑71 (MQ=255) tGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:4264842/1‑70 (MQ=255) tGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:2379489/1‑70 (MQ=255) gCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccg > 1:3609367/1‑70 (MQ=16) gCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccg > 1:233171/1‑70 (MQ=16) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTa < 1:4587399/68‑1 (MQ=255) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:2560355/1‑69 (MQ=255) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:4298404/1‑69 (MQ=255) agcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGa < 1:409901/68‑1 (MQ=255) gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa < 1:3845462/71‑1 (MQ=255) gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa < 1:4628002/71‑1 (MQ=255) caACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtgga < 1:3355794/71‑4 (MQ=12) caACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGa < 1:3765029/66‑1 (MQ=255) caACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCaa < 1:1095740/71‑1 (MQ=255) aaCGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtgg > 1:4200181/1‑67 (MQ=12) aaCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCaa < 1:3928987/70‑1 (MQ=255) cGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAgg > 1:5432914/1‑71 (MQ=255) ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:3842056/1‑69 (MQ=255) ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt > 1:1006632/1‑71 (MQ=255) ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt < 1:1776993/71‑1 (MQ=255) aTCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg > 1:4784177/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:2117113/1‑71 (MQ=255) ccAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:1625862/71‑1 (MQ=255) aGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGa < 1:3901193/70‑1 (MQ=255) aGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt < 1:3479724/71‑1 (MQ=255) ggaGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGAc > 1:1986088/3‑50 (MQ=12) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGc < 1:4687738/70‑1 (MQ=255) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa < 1:3328822/71‑1 (MQ=255) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa < 1:5134130/71‑1 (MQ=255) aGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg > 1:444222/1‑59 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg < 1:165212/52‑1 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAggg < 1:159424/59‑1 (MQ=255) tGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccg > 1:5196927/1‑40 (MQ=12) tGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccg > 1:1841353/1‑40 (MQ=12) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa < 1:809661/47‑1 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:1367844/1‑71 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:2858834/1‑71 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:4119164/1‑71 (MQ=255) ttCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAGGTAAGGGTAATTGTCACGCTAcc > 1:183813/1‑71 (MQ=255) tCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACgg < 1:1061429/66‑1 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAccc < 1:5346171/70‑1 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAccc < 1:3835512/70‑1 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAccc > 1:2931164/1‑70 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCg > 1:515794/1‑71 (MQ=255) tGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCa < 1:2028869/49‑1 (MQ=255) tGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCa < 1:2099292/49‑1 (MQ=255) cACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAgg > 1:1186796/1‑49 (MQ=255) cACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTg < 1:4528965/70‑1 (MQ=255) ccTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATc < 1:4420204/53‑1 (MQ=255) tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATc > 1:342486/1‑51 (MQ=255) tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATc > 1:2754127/1‑51 (MQ=255) gAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa > 1:5319846/1‑71 (MQ=255) gAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa > 1:5314578/1‑71 (MQ=255) aCCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:5263897/45‑1 (MQ=255) aCCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:2689198/45‑1 (MQ=255) cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTgg < 1:3187097/62‑1 (MQ=255) cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTgg < 1:2092730/62‑1 (MQ=255) cTGCGCGATATCAGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTgg < 1:2477612/62‑1 (MQ=255) tGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCa < 1:2324496/37‑1 (MQ=255) tGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:4679938/1‑70 (MQ=255) gcgcGATATCCGCATCAGTACCAGACTCAAAGGTc > 1:5035813/1‑35 (MQ=255) gcgcGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa > 1:5584548/1‑48 (MQ=255) gcgcGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:887521/1‑69 (MQ=255) gcgcGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:5504038/1‑69 (MQ=255) gcgcGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:2221081/1‑69 (MQ=255) cgcgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:3921548/1‑68 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa < 1:3769156/47‑1 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa < 1:4701024/47‑1 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTCCCCGTgg > 1:3660772/1‑54 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt < 1:3029400/57‑1 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt < 1:66138/57‑1 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt < 1:3748658/57‑1 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTag > 1:5381166/1‑66 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGa > 1:2985424/1‑70 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGa < 1:4340702/70‑1 (MQ=255) atCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGtt < 1:68859/63‑1 (MQ=255) tCCTCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg < 1:4604137/38‑1 (MQ=255) tCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg < 1:4202433/38‑1 (MQ=255) tCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg < 1:5204235/38‑1 (MQ=255) tCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg < 1:2836004/38‑1 (MQ=255) tCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGc < 1:3000640/39‑1 (MQ=255) tCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAcc > 1:1789944/1‑47 (MQ=255) tCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAcc > 1:2715166/1‑47 (MQ=255) tCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACAt < 1:4856884/71‑1 (MQ=255) ccGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTag > 1:5508109/1‑63 (MQ=255) cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa > 1:4468722/1‑38 (MQ=255) cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa > 1:5402250/1‑38 (MQ=255) cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg < 1:4864189/70‑1 (MQ=255) cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg < 1:1534703/70‑1 (MQ=255) cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg < 1:2920438/70‑1 (MQ=255) cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGt < 1:4151029/71‑1 (MQ=255) tCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTa < 1:3975569/57‑1 (MQ=255) tCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTa < 1:4892709/57‑1 (MQ=255) tCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACa > 1:772375/1‑70 (MQ=255) tCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACAt > 1:1497699/1‑71 (MQ=255) aGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATc < 1:2565455/70‑1 (MQ=255) aGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCa < 1:5134257/71‑1 (MQ=255) aGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCa < 1:205582/71‑1 (MQ=255) aCCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTg < 1:5667851/40‑1 (MQ=255) aCCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGt > 1:4508417/1‑71 (MQ=255) | TTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGT > Exported/312513‑312652 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |