Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,870,850 T→A 12.2% intergenic (+93/‑87) rnr → / → rlmB exoribonuclease R, RNase R/23S rRNA (Gm2251)‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,870,8500TA12.2% 177.9 / 13.4 65intergenic (+93/‑87)rnr/rlmBexoribonuclease R, RNase R/23S rRNA (Gm2251)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (44/13);  new base A (2/6);  total (46/19)
Fisher's exact test for biased strand distribution p-value = 6.04e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

AGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGT  >  Exported/2870780‑2870919
                                                                      |                                                                     
agGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAt                                                                       <  1:4607426/71‑1 (MQ=255)
    ttAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAAt                                                                    >  1:399353/1‑70 (MQ=255)
    ttAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAAt                                                                    >  1:4549262/1‑70 (MQ=255)
    ttAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAAt                                                                    >  1:1966575/1‑70 (MQ=255)
    ttAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAAt                                                                    >  1:5389853/1‑70 (MQ=255)
     tAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCa                                                                  >  1:3208322/1‑71 (MQ=255)
           gCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCaa                                                             <  1:2078551/70‑1 (MQ=255)
               aCAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAAtt                                                          <  1:4532247/69‑1 (MQ=255)
               aCAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTc                                                         <  1:528715/70‑1 (MQ=255)
                cAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCaa                                                       <  1:737980/71‑1 (MQ=255)
                     tGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAatatat                                                  >  1:4884821/1‑71 (MQ=255)
                     tGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAatatat                                                  <  1:4211964/71‑1 (MQ=255)
                     tGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAatatat                                                  <  1:1370511/71‑1 (MQ=255)
                            tGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCa                                              >  1:1168042/1‑68 (MQ=255)
                              ctCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGAt                                         <  1:238707/69‑1 (MQ=2)
                              ctCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATc                                        <  1:769773/70‑1 (MQ=2)
                                 aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACg                                                                >  1:4954284/1‑45 (MQ=255)
                                 aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCAt                                      >  1:3231049/1‑71 (MQ=255)
                                 aTGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATg                                     <  1:3973050/71‑1 (MQ=2)
                                 aTGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATg                                     <  1:3836467/71‑1 (MQ=2)
                                  tGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATg                                     <  1:4806689/70‑1 (MQ=2)
                                  tGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATg                                     >  1:3543351/1‑70 (MQ=2)
                                  tGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATg                                     <  1:705961/70‑1 (MQ=2)
                                   gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAatata                                                   >  1:1040324/1‑56 (MQ=255)
                                   gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCAt                                      <  1:66238/69‑1 (MQ=255)
                                   gCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATg                                     >  1:4622790/1‑69 (MQ=2)
                                   gCCAGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAatata                                                   >  1:215123/1‑56 (MQ=255)
                                        aTGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCaga                                            >  1:2936130/1‑58 (MQ=255)
                                        aTGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCaga                                            >  1:5062382/1‑58 (MQ=255)
                                        aTGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCaga                                            >  1:5315801/1‑58 (MQ=255)
                                        aTGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGcc                               <  1:2857564/71‑1 (MQ=255)
                                         tGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCaa                                                       >  1:3216614/1‑46 (MQ=255)
                                         tGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGAt                                         >  1:185567/1‑60 (MQ=255)
                                          gcggcgTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCaa                                                       >  1:1504040/1‑45 (MQ=255)
                                                tGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATa                          >  1:4364611/1‑68 (MQ=255)
                                                  aaCGCCTTATCCGGCCTACATAATCACGCAAATTCaa                                                       >  1:2510881/1‑37 (MQ=255)
                                                  aaCGCCTTATCCGGCCTACATAATCACGCAAATTCaa                                                       >  1:2024884/1‑37 (MQ=255)
                                                  aaCGCCTTATCCGGCCTACATAATCACGCAAATTCAat                                                      >  1:5474362/1‑38 (MQ=255)
                                                  aaCGCCTTATCCGGCCTACATAATCACGCAAATTCAat                                                      >  1:2760744/1‑38 (MQ=255)
                                                  aaCGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTa                                   >  1:454123/1‑57 (MQ=255)
                                                  aaCGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACg                      <  1:2576102/70‑1 (MQ=255)
                                                  aaCGCCTTATCCGGCCTACATAATCACGCAAATTAATATATTGCAGAGATCATGTa                                   >  1:2381709/1‑56 (MQ=255)
                                                     gCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGt                  >  1:5519933/1‑71 (MQ=255)
                                                        ttATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTa                                   >  1:4179547/1‑51 (MQ=255)
                                                          aTCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCAt              >  1:3344522/1‑70 (MQ=255)
                                                          aTCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCAt              >  1:3119327/1‑70 (MQ=255)
                                                          aTCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCAt              >  1:3675356/1‑70 (MQ=255)
                                                          aTCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCAt              >  1:645033/1‑70 (MQ=255)
                                                            ccGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAg           >  1:285360/1‑71 (MQ=255)
                                                            ccGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAg           >  1:2819967/1‑71 (MQ=255)
                                                             cGGCCTACATAATCACGCAAATTCAATATATTGCa                                              >  1:3558496/1‑35 (MQ=255)
                                                             cGGCCTACATAATCACGCAAATTCAATATATTGCa                                              >  1:3672851/1‑35 (MQ=255)
                                                             cGGCCTACATAATCACGCAAATTCAATATATTGCa                                              >  1:2983924/1‑35 (MQ=255)
                                                                    cATAATCACGCAAATTCAATATATTGCAGAGATCATGTAgg                                 >  1:4230332/1‑41 (MQ=255)
                                                                    cATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGcc                               >  1:485966/1‑43 (MQ=255)
                                                                    cATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAg                   >  1:648384/1‑55 (MQ=255)
                                                                    cATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAg                   >  1:5458084/1‑55 (MQ=255)
                                                                    cATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCaaaa     >  1:798657/1‑69 (MQ=255)
                                                                    cATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAAc    <  1:5144743/70‑1 (MQ=255)
                                                                    cATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACg   <  1:3719669/71‑1 (MQ=255)
                                                                    cATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACg   <  1:3272388/71‑1 (MQ=255)
                                                                     aTAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACg                      >  1:4076443/1‑51 (MQ=255)
                                                                     aTAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACg                      >  1:376366/1‑51 (MQ=255)
                                                                     aTAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACg                      >  1:2996772/1‑51 (MQ=255)
                                                                     aTAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACg                      >  1:2442516/1‑51 (MQ=255)
                                                                     aTAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGt  <  1:3400942/71‑1 (MQ=255)
                                                                      |                                                                     
AGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGT  >  Exported/2870780‑2870919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: