Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,872,260 G→C 10.9% E171Q (GAA→CAA)  ulaF → L‑ribulose 5‑phosphate 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,872,2600GC10.9% 196.6 / 13.2 74E171Q (GAA→CAA) ulaFL‑ribulose 5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (36/29);  new base C (6/2);  total (42/32)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

GGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAT  >  Exported/2872195‑2872325
                                                                 |                                                                 
ggCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGaaa                                                                <  1:431422/69‑1 (MQ=255)
    aCGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCt                                                          >  1:1056150/1‑71 (MQ=255)
     cGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCt                                                          >  1:412421/1‑70 (MQ=255)
      gCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCa                                                        >  1:3175748/1‑71 (MQ=255)
      gCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCa                                                        >  1:2834798/1‑71 (MQ=255)
      gCCGAGCCGCTGCATACGCCGGCAATTGTGGTGTATCAGCACGGGCCGTTGGCCTGGGGGAAAGATGCTCa                                                        >  1:2461073/1‑71 (MQ=255)
       ccGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCAc                                                       <  1:943868/71‑1 (MQ=255)
       ccGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCAc                                                       <  1:157381/71‑1 (MQ=255)
           gCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTc                                                         >  1:5437778/1‑65 (MQ=255)
           gCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATg                                                   >  1:2041557/1‑71 (MQ=255)
                 gCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGaaa                                                                >  1:2585054/1‑52 (MQ=255)
                   aTACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCAt                                           <  1:2226036/71‑1 (MQ=255)
                   aTACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCAt                                           <  1:3846571/71‑1 (MQ=255)
                   aTACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCAt                                           <  1:3823442/71‑1 (MQ=255)
                    tACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCAt                                           >  1:875985/1‑70 (MQ=255)
                            gAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATg                                                   >  1:5521392/1‑54 (MQ=255)
                            gAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATg                                                   >  1:22326/1‑54 (MQ=255)
                             aaTTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGaaa                                                                <  1:4718489/40‑1 (MQ=255)
                               ttGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCggtggt                                >  1:2306317/1‑70 (MQ=255)
                               ttGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCggtggt                                >  1:923546/1‑70 (MQ=255)
                                 gtggtgTATCAGCACGGGCCGTTCGCCTGGGGGAAAg                                                               >  1:2071874/1‑37 (MQ=255)
                                 gtggtgTATCAGCACGGGCCGTTCGCCTGGGGGAAAg                                                               >  1:3587105/1‑37 (MQ=255)
                                   ggtgTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATg                            <  1:2067678/70‑1 (MQ=255)
                                    gtgtATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATgg                           <  1:2211007/70‑1 (MQ=255)
                                     tgtATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCAc                                                       <  1:1700472/41‑1 (MQ=255)
                                       tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCa                                                        <  1:1127238/38‑1 (MQ=255)
                                       tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCa                                                        <  1:3323955/38‑1 (MQ=255)
                                       tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGc                                                  <  1:2000538/44‑1 (MQ=255)
                                       tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCggtgg                                 >  1:4805118/1‑61 (MQ=255)
                                       tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCggtgg                                 >  1:2581399/1‑61 (MQ=255)
                                       tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCggtgg                                 >  1:3860845/1‑61 (MQ=255)
                                       tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGgaaga                       <  1:4604790/71‑1 (MQ=255)
                                       tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGgaag                        >  1:4285458/1‑70 (MQ=255)
                                         tCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAAcgcg                                     >  1:1051697/1‑55 (MQ=255)
                                         tCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAAcgcg                                     >  1:1819863/1‑55 (MQ=255)
                                         tCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGgaagaa                      <  1:4547312/70‑1 (MQ=255)
                                           cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGgaa                         >  1:848685/3‑65 (MQ=255)
                                           cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGtc                   >  1:4479915/3‑70 (MQ=255)
                                           cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGtc                   >  1:2887968/3‑70 (MQ=255)
                                           cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGt                    >  1:1172157/3‑70 (MQ=255)
                                           aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                    <  1:4557762/70‑1 (MQ=255)
                                           aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                   <  1:4289954/71‑1 (MQ=255)
                                           aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                   >  1:957472/1‑71 (MQ=255)
                                           aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                   >  1:374889/1‑71 (MQ=255)
                                           aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                   <  1:3622580/71‑1 (MQ=255)
                                            ccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGtc                   >  1:3668662/2‑69 (MQ=255)
                                             cACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGTCGc                 <  1:1034563/71‑1 (MQ=255)
                                              aCGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                    >  1:5679899/1‑67 (MQ=255)
                                              aCGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                    >  1:3283436/1‑67 (MQ=255)
                                              aCGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGTCGct                >  1:648511/1‑70 (MQ=255)
                                              aCGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGTCGct                <  1:744477/71‑2 (MQ=255)
                                              aCGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAAcgccg                                    <  1:2246496/51‑3 (MQ=255)
                                               cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCg                >  1:3421720/1‑70 (MQ=255)
                                               cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGa               <  1:394521/71‑1 (MQ=255)
                                               cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGa               <  1:5533142/71‑1 (MQ=255)
                                                gggCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGa               >  1:80711/1‑70 (MQ=255)
                                                  gCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGAt                             >  1:4939775/1‑54 (MQ=255)
                                                  gCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaaa            <  1:4340449/71‑1 (MQ=255)
                                                  gCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaaa            >  1:209393/1‑71 (MQ=255)
                                                   ccGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaaa            <  1:4346794/70‑1 (MQ=255)
                                                     gTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCAt                                           <  1:3178707/37‑1 (MQ=255)
                                                     gTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCAt                                           <  1:4101342/37‑1 (MQ=255)
                                                     gTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATgg         >  1:1590732/1‑71 (MQ=255)
                                                      ttCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCggtggt                                <  1:3008695/47‑1 (MQ=255)
                                                        cGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCgg                                    >  1:3268981/1‑41 (MQ=255)
                                                        cGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCgg                                    >  1:3264103/1‑41 (MQ=255)
                                                        cGCCTGGGGGAAAGATGCTCAAGATGCGGTGCATAACGCGGTGGTGa                              >  1:3726122/1‑47 (MQ=255)
                                                         gCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg     <  1:4904463/71‑1 (MQ=255)
                                                         gCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg     <  1:4897729/71‑1 (MQ=255)
                                                         gCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg     <  1:4288885/71‑1 (MQ=255)
                                                          ccTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGgaaga                       <  1:3710590/52‑1 (MQ=255)
                                                            tGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAt  >  1:5132382/1‑71 (MQ=255)
                                                            tGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAt  >  1:351066/1‑71 (MQ=255)
                                                            tGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAt  >  1:3644140/1‑71 (MQ=255)
                                                             gggggAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                    <  1:1929678/52‑1 (MQ=255)
                                                              ggggAAAGATGCTCACGATGCGGTGCATAACGCggtggt                                <  1:5049914/39‑1 (MQ=255)
                                                              ggggAAAGATGCTCACGATGCGGTGCATAACGCggtggt                                <  1:4909594/39‑1 (MQ=255)
                                                              ggggAAAGATGCTCACGATGCGGTGCATAACGCggtggt                                <  1:2546126/39‑1 (MQ=255)
                                                              ggggAAAGATGCTCACGATGCGGTGCATAACGCggtggt                                <  1:1243594/39‑1 (MQ=255)
                                                              ggggAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAt  <  1:1433365/69‑1 (MQ=255)
                                                                 |                                                                 
GGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAT  >  Exported/2872195‑2872325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑