Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,872,297 A→C 8.5% D183A (GAT→GCT)  ulaF → L‑ribulose 5‑phosphate 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,872,2970AC 8.5% 235.5 / 15.2 94D183A (GAT→GCT) ulaFL‑ribulose 5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (30/56);  new base C (6/2);  total (36/58)
Fisher's exact test for biased strand distribution p-value = 5.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

GGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGA  >  Exported/2872230‑2872361
                                                                   |                                                                
ggtgTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATg                                                                <  1:2067678/70‑1 (MQ=255)
 gtgtATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATgg                                                               <  1:2211007/70‑1 (MQ=255)
    tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGgaaga                                                           <  1:4604790/71‑1 (MQ=255)
    tATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGgaag                                                            >  1:4285458/1‑70 (MQ=255)
      tCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGgaagaa                                                          <  1:4547312/70‑1 (MQ=255)
        cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGgaa                                                             >  1:848685/3‑65 (MQ=255)
        cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGtc                                                       >  1:4479915/3‑70 (MQ=255)
        cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGtc                                                       >  1:2887968/3‑70 (MQ=255)
        cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGt                                                        >  1:1172157/3‑70 (MQ=255)
        aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                        <  1:4557762/70‑1 (MQ=255)
        aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                                                       >  1:957472/1‑71 (MQ=255)
        aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                                                       <  1:4289954/71‑1 (MQ=255)
        aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                                                       >  1:374889/1‑71 (MQ=255)
        aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                                                       <  1:3622580/71‑1 (MQ=255)
         ccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGtc                                                       >  1:3668662/2‑69 (MQ=255)
          cACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGTCGc                                                     <  1:1034563/71‑1 (MQ=255)
           aCGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                        >  1:3283436/1‑67 (MQ=255)
           aCGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                        >  1:5679899/1‑67 (MQ=255)
           aCGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGTCGct                                                    >  1:648511/1‑70 (MQ=255)
           aCGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGTCGct                                                    <  1:744477/71‑2 (MQ=255)
            cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCg                                                    >  1:3421720/1‑70 (MQ=255)
            cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGa                                                   <  1:394521/71‑1 (MQ=255)
            cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGa                                                   <  1:5533142/71‑1 (MQ=255)
             gggCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGa                                                   >  1:80711/1‑70 (MQ=255)
               gCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGAt                                                                 >  1:4939775/1‑54 (MQ=255)
               gCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaaa                                                <  1:4340449/71‑1 (MQ=255)
               gCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaaa                                                >  1:209393/1‑71 (MQ=255)
                ccGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaaa                                                <  1:4346794/70‑1 (MQ=255)
                  gTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATgg                                             >  1:1590732/1‑71 (MQ=255)
                     cGCCTGGGGGAAAGATGCTCAAGATGCGGTGCATAACGCGGTGGTGa                                                                  >  1:3726122/1‑47 (MQ=255)
                      gCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                         <  1:4897729/71‑1 (MQ=255)
                      gCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                         <  1:4288885/71‑1 (MQ=255)
                      gCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                         <  1:4904463/71‑1 (MQ=255)
                       ccTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGgaaga                                                           <  1:3710590/52‑1 (MQ=255)
                         tGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAt                                      >  1:3644140/1‑71 (MQ=255)
                         tGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAt                                      >  1:5132382/1‑71 (MQ=255)
                         tGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAt                                      >  1:351066/1‑71 (MQ=255)
                          gggggAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                        <  1:1929678/52‑1 (MQ=255)
                           ggggAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAt                                      <  1:1433365/69‑1 (MQ=255)
                               aaaGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                        <  1:1849441/47‑1 (MQ=255)
                                aaGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCcgc                               >  1:490196/1‑71 (MQ=255)
                                 aGATTCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                        <  1:4170256/45‑1 (MQ=255)
                                 aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                        <  1:4422703/45‑1 (MQ=255)
                                 aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                        <  1:429674/45‑1 (MQ=255)
                                 aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                        <  1:2794660/45‑1 (MQ=255)
                                 aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTgg                                                      >  1:148463/1‑47 (MQ=255)
                                 aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTgg                                                      >  1:3316616/1‑47 (MQ=255)
                                 aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTgg                                                      >  1:2661147/1‑47 (MQ=255)
                                 aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTgg                                                      >  1:4602987/1‑47 (MQ=255)
                                 aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaa                                                  <  1:4716890/51‑1 (MQ=255)
                                 aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaa                                                  <  1:1144192/51‑1 (MQ=255)
                                 aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaa                                                  <  1:1545982/51‑1 (MQ=255)
                                 aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaa                                                  <  1:4427771/51‑1 (MQ=255)
                                   aTGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCgg                             >  1:4235227/1‑70 (MQ=255)
                                   aTGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCgg                             >  1:1123374/1‑70 (MQ=255)
                                     gCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAt                          >  1:2695070/1‑71 (MQ=255)
                                      cTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt                         <  1:2629783/71‑1 (MQ=255)
                                      cTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAt                          <  1:796782/70‑1 (MQ=255)
                                       tCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt                         >  1:2634633/1‑70 (MQ=255)
                                       tCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAt                          <  1:66643/69‑1 (MQ=255)
                                         aCGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTaa                       <  1:1990982/70‑1 (MQ=255)
                                         aCGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAAc                      >  1:803648/1‑71 (MQ=255)
                                         aCGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAAc                      >  1:3027528/1‑71 (MQ=255)
                                          cGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                         <  1:1080159/51‑1 (MQ=255)
                                          cGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                         <  1:1450193/51‑1 (MQ=255)
                                          cGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                         <  1:278121/51‑1 (MQ=255)
                                            aTGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCca                   >  1:3285736/1‑71 (MQ=255)
                                            aTGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCca                   >  1:5442204/1‑71 (MQ=255)
                                             tGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcac                  <  1:5400436/71‑1 (MQ=255)
                                               cGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaa                                                  <  1:3606104/37‑1 (MQ=255)
                                               cGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCcgc                               <  1:3874169/56‑1 (MQ=255)
                                               cGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCcgc                               <  1:479265/56‑1 (MQ=255)
                                               aggTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaa                                                  <  1:2204294/36‑1 (MQ=255)
                                                ggTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAAc               <  1:4748852/71‑1 (MQ=255)
                                                 gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt                         <  1:603351/60‑1 (MQ=255)
                                                 gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt                         <  1:593340/60‑1 (MQ=255)
                                                 gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt                         <  1:5330901/60‑1 (MQ=255)
                                                 gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcac                  <  1:1541497/67‑1 (MQ=255)
                                                 gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcac                  <  1:2913053/67‑1 (MQ=255)
                                                 gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcac                  <  1:5342703/67‑1 (MQ=255)
                                                  tGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAATTGGCGTg                                         <  1:4648006/43‑1 (MQ=255)
                                                  tGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                         <  1:1786805/43‑1 (MQ=255)
                                                  tGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                         <  1:80033/43‑1 (MQ=255)
                                                  tGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                         <  1:3872119/43‑1 (MQ=255)
                                                  tGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                         <  1:4825754/43‑1 (MQ=255)
                                                    cATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa            <  1:4491733/70‑1 (MQ=255)
                                                        aCGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGccc                                 >  1:4066239/1‑45 (MQ=255)
                                                         cgcgGTTGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATcaca      <  1:1232952/71‑1 (MQ=255)
                                                         cgcgGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt                         <  1:1778596/52‑1 (MQ=255)
                                                              tggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcaca                 <  1:2918272/55‑1 (MQ=255)
                                                              tggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcaca                 <  1:1449565/55‑1 (MQ=255)
                                                              tggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcaca                 <  1:876268/55‑1 (MQ=255)
                                                              tggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGa  >  1:4586885/1‑70 (MQ=255)
                                                               ggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGa  >  1:2162000/1‑69 (MQ=255)
                                                                  gATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCcga                               >  1:1944767/1‑36 (MQ=255)
                                                                   |                                                                
GGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGA  >  Exported/2872230‑2872361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: