Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,891,414 C→A 83.3% P23P (CCC→CCA ytfQ → predicted sugar transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,891,4140CA83.3% 134.5 / 29.2 72P23P (CCC→CCAytfQpredicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (3/9);  new base A (35/25);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 5.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

GTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGA  >  Exported/2891348‑2891481
                                                                  |                                                                   
gtgGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTaa                                                                 >  1:3160357/1‑71 (MQ=255)
    aaaCGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGt                                                             <  1:2771445/71‑1 (MQ=255)
     aaCGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGtt                                                            <  1:2156869/71‑1 (MQ=255)
       cGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTg                                                           >  1:793573/1‑70 (MQ=255)
        gCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGa                                                         >  1:477223/1‑71 (MQ=255)
        gCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGa                                                         >  1:1644834/1‑71 (MQ=255)
            acttaTAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGAttt                                                      <  1:499771/70‑1 (MQ=255)
            acttaTAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGAttt                                                      >  1:3172682/1‑70 (MQ=255)
               tataGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTc                                                    >  1:1988448/1‑69 (MQ=255)
               tataGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCg                                                   >  1:856054/1‑70 (MQ=255)
               tataGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCg                                                   >  1:4192863/1‑70 (MQ=255)
               tataGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCg                                                   >  1:4080021/1‑70 (MQ=255)
                   gTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGAt                                                        >  1:2968793/1‑61 (MQ=255)
                   gTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGt                                              <  1:318984/71‑1 (MQ=255)
                    tctcTGCAGTCTCGGCAGCCATGTCGTCTTTGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAgg                                               <  1:5673827/69‑1 (MQ=255)
                    tctcTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCg                                                              <  1:184488/54‑1 (MQ=255)
                    tctcTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCg                                                              <  1:3869589/54‑1 (MQ=255)
                    tctcTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAgg                                               <  1:3371476/69‑1 (MQ=255)
                    tctcTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAgg                                               <  1:1667076/69‑1 (MQ=255)
                     ctctGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGtcg                                            >  1:3043732/1‑71 (MQ=255)
                      tctGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGtcg                                            >  1:3711553/1‑70 (MQ=255)
                        tGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGAtttt                                                     >  1:1527808/1‑59 (MQ=255)
                        tGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGAtttt                                                     >  1:2525782/1‑59 (MQ=255)
                        tGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGAtt                                                       <  1:5429644/57‑1 (MQ=255)
                          cAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGtcggat                                         <  1:1386304/69‑1 (MQ=255)
                               tcGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTgg                                                          >  1:399904/1‑47 (MQ=255)
                               tcGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTgg                                                          >  1:2915460/1‑47 (MQ=255)
                               tcGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTgg                                                          >  1:5580854/1‑47 (MQ=255)
                               tcGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTg                                                           >  1:2240297/1‑46 (MQ=255)
                               tcGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGa                                                         >  1:1125283/1‑48 (MQ=255)
                                cGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGt                                              <  1:3673012/58‑1 (MQ=255)
                                 ggCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCa                                 <  1:3736948/70‑1 (MQ=255)
                                  gCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGc                                                  >  1:5578602/1‑52 (MQ=255)
                                  gCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAgg                               <  1:4750902/71‑1 (MQ=255)
                                    aGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAg                                                >  1:561895/1‑52 (MQ=255)
                                    aGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAg                                                >  1:1052720/1‑52 (MQ=255)
                                         tGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGAtt                                                       <  1:5673746/40‑1 (MQ=255)
                                         tGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGAtt                                                       <  1:5360310/40‑1 (MQ=255)
                                         tGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGAtt                                                       <  1:1094312/40‑1 (MQ=255)
                                          gtcgtcTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATc                                  <  1:606165/60‑1 (MQ=255)
                                          gtcgtcTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATc                                  <  1:4242425/60‑1 (MQ=255)
                                           tcgtcTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAg                                                >  1:1013643/1‑45 (MQ=255)
                                           tcgtcTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGc                      >  1:1054248/1‑71 (MQ=255)
                                           tcgtcTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGc                      >  1:5503427/1‑71 (MQ=255)
                                           tcgtcTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCAGGCGTGc                      >  1:3999523/1‑71 (MQ=255)
                                           tcgtcTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTAGCAGGTCGGATCGGAATCAGGCTGGCGTGc                      >  1:4359971/1‑71 (MQ=255)
                                             gtcTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGcc                     <  1:195057/70‑1 (MQ=255)
                                             gtcTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGcc                     <  1:623359/70‑1 (MQ=255)
                                             gtcTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGcc                     <  1:3563001/70‑1 (MQ=255)
                                             gtcTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCg                    <  1:4976571/71‑1 (MQ=255)
                                               cTATGGCGTTGGCCGCTCCATTAACCGTTGGAtttt                                                     <  1:2868340/36‑1 (MQ=255)
                                                  tGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCTGAATCAGGCTGGCGTGCCGCa                  <  1:2404115/68‑1 (MQ=255)
                                                  tGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCa                  <  1:1158279/68‑1 (MQ=255)
                                                  tGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGa                >  1:4566704/1‑70 (MQ=255)
                                                  tGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGa                <  1:254359/70‑1 (MQ=255)
                                                  tGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGa                <  1:4288357/70‑1 (MQ=255)
                                                  tGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGa                <  1:3900937/70‑1 (MQ=255)
                                                   ggCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGa                <  1:3805718/69‑1 (MQ=255)
                                                   ggCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCa                                                 >  1:115211/1‑36 (MQ=255)
                                                   ggCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGc                      >  1:3144295/1‑63 (MQ=255)
                                                   ggCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGc                      >  1:4882948/1‑63 (MQ=255)
                                                    gCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAAt                                   >  1:664518/1‑49 (MQ=255)
                                                    gCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAAt                                   >  1:5448093/1‑49 (MQ=255)
                                                    gCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGaaa              <  1:300072/70‑1 (MQ=255)
                                                       ttGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAAt                                   <  1:2084694/46‑1 (MQ=255)
                                                         ggCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGaa               >  1:1389528/1‑64 (MQ=255)
                                                         ggCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAAtg        >  1:2040143/1‑71 (MQ=255)
                                                         ggCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAAtg        >  1:2671392/1‑71 (MQ=255)
                                                         ggCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAAtg        >  1:135331/1‑71 (MQ=255)
                                                            cGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGa                <  1:2175515/60‑1 (MQ=255)
                                                             gCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGa                >  1:3592812/1‑59 (MQ=255)
                                                              cTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCg   <  1:5172715/71‑1 (MQ=255)
                                                                cccTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGa  >  1:2678997/1‑70 (MQ=255)
                                                                cccTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGa  >  1:251989/1‑70 (MQ=255)
                                                                cccTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGa  >  1:2543319/1‑70 (MQ=255)
                                                                  |                                                                   
GTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGA  >  Exported/2891348‑2891481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: