Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 312,705 T→G 20.0% T56T (ACA→ACC acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported312,7050TG20.0% 124.4 / 38.9 75T56T (ACA→ACCacrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (26/34);  new base G (6/9);  total (32/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

GGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGC  >  Exported/312636‑312774
                                                                     |                                                                     
ggACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTg                                                                      <  1:4116180/71‑1 (MQ=255)
ggACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTg                                                                      <  1:277181/71‑1 (MQ=255)
  aCATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGtt                                                                    <  1:3322075/71‑1 (MQ=255)
   cATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt                                                                   <  1:3734500/71‑1 (MQ=255)
   cATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt                                                                   <  1:2185003/71‑1 (MQ=255)
    aTGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGtttt                                                                  >  1:1843486/1‑71 (MQ=255)
       tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCg                                                                <  1:4256768/70‑1 (MQ=255)
       tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCg                                                                <  1:4250009/70‑1 (MQ=255)
       tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCg                                                                <  1:3021689/70‑1 (MQ=255)
       tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGGCCTGCACTGtttt                                                                  <  1:2766564/68‑1 (MQ=255)
         cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt                                                             <  1:1173121/71‑1 (MQ=255)
         cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt                                                             >  1:35407/1‑71 (MQ=255)
         cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt                                                             <  1:2673768/71‑1 (MQ=255)
         cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt                                                             <  1:1243728/71‑1 (MQ=255)
           tCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATc                                                            <  1:4368823/70‑1 (MQ=37)
            cAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGt                                                                     >  1:1745536/1‑60 (MQ=37)
            cAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGt                                                                     >  1:1705101/1‑60 (MQ=37)
            cAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGt                                                                     >  1:3756695/1‑60 (MQ=37)
             aGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAg                                                          <  1:5294707/70‑1 (MQ=255)
             aGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAg                                                          <  1:3614084/70‑1 (MQ=255)
             aGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAg                                                          <  1:3307584/70‑1 (MQ=255)
               gTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAgcgc                                                       <  1:3808628/71‑1 (MQ=255)
                 tATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCg                                                     >  1:979089/1‑71 (MQ=255)
                 tATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCg                                                     >  1:5689768/1‑71 (MQ=255)
                 tATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCg                                                     >  1:2702726/1‑71 (MQ=255)
                  aTCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGtc                                                                    >  1:1652766/1‑54 (MQ=37)
                    cGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCa                                                              >  1:248889/1‑59 (MQ=25)
                    agATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg                                                  >  1:3245035/2‑71 (MQ=255)
                     gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg                                                  <  1:5188206/70‑1 (MQ=255)
                     gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg                                                  <  1:4475126/70‑1 (MQ=255)
                     gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg                                                  <  1:1161302/70‑1 (MQ=255)
                     gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg                                                  <  1:269216/70‑1 (MQ=255)
                     gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg                                                  <  1:125038/70‑1 (MQ=255)
                     gATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGt                                                 <  1:388676/71‑1 (MQ=255)
                     gATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGTTCTGCGCATCAGCGCCcgga                                                  <  1:5384541/70‑2 (MQ=255)
                     gATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCcggt                                                  <  1:1290593/70‑2 (MQ=255)
                      aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt                                                                   >  1:2680137/1‑52 (MQ=255)
                      aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt                                                                   >  1:4070017/1‑52 (MQ=255)
                      aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt                                                                   >  1:45095/1‑52 (MQ=255)
                      aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg                                                  >  1:2838786/1‑69 (MQ=255)
                        aCCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTag                                               >  1:1469681/1‑70 (MQ=255)
                         ccGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTc                                                                 >  1:1595539/1‑51 (MQ=255)
                          cGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATagttt                                            <  1:3017300/71‑4 (MQ=255)
                           gTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTaggagg                                           >  1:3180705/1‑71 (MQ=255)
                            ttCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGcc                                                      <  1:3628965/59‑1 (MQ=255)
                               atatTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCgg                                        <  1:2095546/70‑1 (MQ=255)
                                tatTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATagt                                              <  1:3632931/63‑2 (MQ=255)
                                  ttCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCa                                                           >  1:4439182/1‑48 (MQ=255)
                                  ttCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTagga                                             >  1:3892778/1‑62 (MQ=255)
                                  ttCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATc                                                            <  1:3153770/47‑1 (MQ=255)
                                   tCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATagtt                                             >  1:1346194/1‑59 (MQ=255)
                                    cTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATc                                   <  1:180377/70‑1 (MQ=255)
                                    cTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCg                                  >  1:2623153/1‑71 (MQ=255)
                                         cGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGgag                                      <  1:2043722/62‑1 (MQ=255)
                                         cGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAg                                                          <  1:1227074/42‑1 (MQ=255)
                                          gATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTa                               >  1:356855/1‑68 (MQ=255)
                                           aTAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATag                                               <  1:1605184/51‑1 (MQ=255)
                                           aTAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATag                                               <  1:2063184/51‑1 (MQ=255)
                                               ccTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGt                                 <  1:3274199/61‑1 (MQ=255)
                                                  gtgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCcggcgg                     >  1:2464280/1‑70 (MQ=255)
                                                   tgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCcggc                       <  1:758756/67‑1 (MQ=255)
                                                   tgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGt                    <  1:3920667/70‑1 (MQ=255)
                                                   tgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATAGTTACTGCCGGCGGt                    <  1:4952196/70‑1 (MQ=255)
                                                       aCCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAAt               >  1:1350858/1‑71 (MQ=255)
                                                       aCCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAAt               >  1:4572112/1‑71 (MQ=255)
                                                          gtgtCCTGCACTGTTTTCGCATCAGCGCCGGTGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGt            <  1:4952369/71‑1 (MQ=255)
                                                          gtgtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCg             >  1:1962058/1‑70 (MQ=255)
                                                           tgtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTa                               >  1:2581657/1‑51 (MQ=255)
                                                               cTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATc                                   >  1:3605734/1‑43 (MQ=255)
                                                               cTGCACTGTTTTCGCATCAGCGCCGGGGTAGCAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGa        <  1:2107639/70‑1 (MQ=255)
                                                                 gCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGt                                 <  1:4156464/43‑1 (MQ=255)
                                                                 gCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGatat     >  1:2573190/1‑71 (MQ=255)
                                                                  cACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATAtt    <  1:2573076/71‑1 (MQ=255)
                                                                  cACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATAtt    >  1:1729493/1‑71 (MQ=255)
                                                                    cTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTgc  >  1:969068/1‑71 (MQ=255)
                                                                     |                                                                     
GGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGTAGGATATTGC  >  Exported/312636‑312774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: