BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	313,552	C→T	8.2%	P179P (CCG→CCA)	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	313,552	0	C	T	8.2%	317.2 / 12.4	110	P179P (CCG→CCA)	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand): ref base C (53/48); new base T (2/7); total (55/55)
Fisher's exact test for biased strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 113 total aligned reads displayed.

GCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTT  >  Exported/313482‑313618

gcgcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATc                                                                    >  1:5354033/1‑71 (MQ=255)

  gcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATc                                                                    >  1:3150097/1‑69 (MQ=255)

  gcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCgg                                                                  <  1:2841279/71‑1 (MQ=255)

   cAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                                 <  1:2631577/71‑1 (MQ=255)

   cAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGA‑CGCTAATCGga                                                                 <  1:4390026/70‑1 (MQ=255)

     gTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                                 >  1:1120198/1‑69 (MQ=255)

        gCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAgg                                                             <  1:4347586/70‑1 (MQ=255)

             aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGa                                                          >  1:1387643/1‑68 (MQ=255)

             aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACtt                                                       >  1:1000774/1‑71 (MQ=255)

             aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACt                                                        <  1:4078213/70‑1 (MQ=255)

              ccGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCgg                                                                  <  1:2058600/59‑1 (MQ=255)

                gTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGgag                                                                >  1:2922155/1‑59 (MQ=255)

                 ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACttt                                                      <  1:1507394/68‑1 (MQ=255)

                 ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACttt                                                      <  1:3908390/68‑1 (MQ=255)

                 ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                                   >  1:1044453/1‑71 (MQ=255)

                 ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                                   >  1:4168834/1‑71 (MQ=255)

                 ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                                   >  1:1079468/1‑71 (MQ=255)

                  tCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                                  >  1:284897/1‑71 (MQ=255)

                  tCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                                  >  1:278007/1‑71 (MQ=255)

                      tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                                 <  1:3958640/68‑1 (MQ=255)

                      tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTa                                                <  1:1234323/69‑1 (MQ=255)

                      tACCAATGCGCCTTCCGTCACGTGCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                                 <  1:2560667/68‑1 (MQ=255)

                       aCCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGc                                             >  1:4053291/1‑71 (MQ=255)

                          aaTGCGGCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAg                                          <  1:4957902/71‑1 (MQ=255)

                            tGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                                  >  1:2424949/1‑61 (MQ=255)

                             gcgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                                 >  1:877363/1‑45 (MQ=255)

                              cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                                 >  1:609626/1‑44 (MQ=255)

                              cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                                 >  1:885729/1‑44 (MQ=255)

                              cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                       <  1:555686/70‑1 (MQ=255)

                              cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                       >  1:5604312/1‑70 (MQ=255)

                              cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                                      <  1:3291585/71‑1 (MQ=255)

                               gcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                                      <  1:345774/70‑1 (MQ=255)

                                 cTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATc                                   >  1:5429045/1‑71 (MQ=255)

                                  ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                             <  1:410310/60‑1 (MQ=255)

                                  ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                             <  1:4492323/60‑1 (MQ=255)

                                  ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                                             <  1:5609237/60‑1 (MQ=255)

                                  ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGAt                                    <  1:2698399/69‑1 (MQ=255)

                                  ttCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAg                                          <  1:333139/63‑1 (MQ=255)

                                  ttCCGTCACGTTCGACTTACCAATACGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATc                                   >  1:1768711/1‑70 (MQ=255)

                                   tcggTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGa                                     >  1:1674694/4‑67 (MQ=255)

                                    ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgc                                >  1:5309105/1‑71 (MQ=255)

                                    ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgc                                >  1:747901/1‑71 (MQ=255)

                                     cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                               >  1:3528495/1‑71 (MQ=255)

                                     cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                               <  1:2231851/71‑1 (MQ=255)

                                     cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                               <  1:2819622/71‑1 (MQ=255)

                                      gTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                                     <  1:1119593/48‑1 (MQ=255)

                                      gTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                                     <  1:4627500/48‑1 (MQ=255)

                                       tCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                                 <  1:5474630/51‑1 (MQ=255)

                                       tCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                             <  1:2370381/71‑1 (MQ=255)

                                        cACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGa                                         >  1:105230/1‑58 (MQ=255)

                                        cACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAg                            >  1:1840239/1‑71 (MQ=255)

                                         aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                                      >  1:3201007/1‑60 (MQ=255)

                                         aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGAt                                    >  1:5252971/1‑62 (MQ=255)

                                         aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGt                           >  1:3249714/1‑71 (MQ=255)

                                            ttCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                        <  1:5258923/71‑1 (MQ=255)

                                             tCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGAtt                                       <  1:905778/55‑1 (MQ=255)

                                             tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                       >  1:3141002/1‑55 (MQ=255)

                                             tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                       >  1:3766842/1‑55 (MQ=255)

                                             tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                       >  1:3422879/1‑55 (MQ=255)

                                             tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                                       >  1:2593473/1‑55 (MQ=255)

                                             tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGa                                     >  1:3952600/1‑57 (MQ=255)

                                              cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                                     >  1:28298/1‑40 (MQ=255)

                                              cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                      >  1:717827/1‑71 (MQ=255)

                                                 cTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCa                                           <  1:3053031/47‑1 (MQ=255)

                                                 cTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCTCGCAGTTTCAAc                     <  1:3527718/69‑1 (MQ=255)

                                                    aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                      >  1:2968328/1‑65 (MQ=255)

                                                    aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                      >  1:1506094/1‑65 (MQ=255)

                                                    aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAg                >  1:1059598/1‑71 (MQ=255)

                                                      cAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCt              <  1:3454192/71‑1 (MQ=255)

                                                       aaTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCtt             <  1:5120344/71‑1 (MQ=255)

                                                        aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                        <  1:5623541/59‑1 (MQ=255)

                                                        aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                        <  1:4571945/59‑1 (MQ=255)

                                                        aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc                        <  1:1477636/59‑1 (MQ=255)

                                                         tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                               >  1:1829267/1‑51 (MQ=255)

                                                         tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTc           <  1:4584586/71‑1 (MQ=255)

                                                         tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGAACcgcga                              <  1:3967292/52‑2 (MQ=255)

                                                          gCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                      >  1:2261863/1‑59 (MQ=255)

                                                          gCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                      >  1:712816/1‑59 (MQ=255)

                                                          gCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcg          >  1:5637393/1‑71 (MQ=255)

                                                           cGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGa                                         >  1:5145249/1‑39 (MQ=255)

                                                           cGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGa                                         >  1:3357281/1‑39 (MQ=255)

                                                           cGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCa                 >  1:3694373/1‑63 (MQ=255)

                                                             aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                             <  1:823128/49‑1 (MQ=255)

                                                             aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                             <  1:4850801/49‑1 (MQ=255)

                                                             aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa                             <  1:2506763/49‑1 (MQ=255)

                                                             aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccg       <  1:3844898/71‑1 (MQ=255)

                                                              ccGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccgc      <  1:4261138/71‑1 (MQ=255)

                                                              ccGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccg       >  1:159968/1‑70 (MQ=255)

                                                               cGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGATGCGAGCGCTTTCGACTGTGGCTTTtgcggc      <  1:3684844/70‑1 (MQ=255)

                                                               cGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCa     <  1:137215/71‑1 (MQ=255)

                                                                gCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGATGCGAGCGCTTTCGACTGTGGCTTTTGCGGCaa    <  1:2618231/71‑2 (MQ=255)

                                                                gCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccgc      <  1:5635521/69‑1 (MQ=255)

                                                                gCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccgc      <  1:5641863/69‑1 (MQ=255)

                                                                gCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgcc        <  1:4520171/67‑1 (MQ=255)

                                                                  tAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccgc      >  1:4573751/1‑67 (MQ=255)

                                                                  tAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTcgccgc      >  1:3958319/1‑67 (MQ=255)

                                                                  tAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGt   <  1:4904374/70‑1 (MQ=255)

                                                                    aTCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGtt  <  1:2694304/69‑1 (MQ=255)

                                                                     tCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAg                <  1:418016/54‑1 (MQ=255)

                                                                     tCGGAGAGGTGACTTAGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa                      >  1:2007864/1‑48 (MQ=255)

GCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTT  >  Exported/313482‑313618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`