Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 2,952,864 | G→A | 9.3% | intergenic (‑173/+95) | leuQ ← / ← rsmC | tRNA‑Leu/16S RNA m2G1207 methylase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 2,952,864 | 0 | G | A | 9.3% | 121.4 / 2.2 | 43 | intergenic (‑173/+95) | leuQ/rsmC | tRNA‑Leu/16S RNA m2G1207 methylase |
Reads supporting (aligned to +/- strand): ref base G (19/20); new base A (2/2); total (21/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCA > Exported/2952795‑2952933 | aaaTGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGgc < 1:3931403/71‑1 (MQ=255) aaaTGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGgc > 1:1764167/1‑71 (MQ=255) cATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCtt < 1:3172218/71‑1 (MQ=255) aTCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTa > 1:3616736/1‑71 (MQ=255) aTCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTa > 1:1612340/1‑71 (MQ=255) cAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGTGGCAGATGCCAGATGCGACGCTGGCGCGTCTTAt > 1:4035718/1‑70 (MQ=255) aGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCt < 1:1496207/71‑1 (MQ=255) gAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTgg > 1:5601990/1‑71 (MQ=255) aaaCTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGc > 1:2654080/1‑71 (MQ=255) aaCTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGcc > 1:3093730/1‑71 (MQ=255) gCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACg > 1:824589/1‑70 (MQ=255) gCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACg < 1:1793427/70‑1 (MQ=255) cGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACg > 1:4445936/1‑69 (MQ=255) aGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCt < 1:1797335/71‑1 (MQ=255) aGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCt < 1:1054707/71‑1 (MQ=255) ggTTTGGTATGCATGCGGTGGCAGATGCCAGATGCGACGCTGGCGCGTCt < 1:5191639/50‑1 (MQ=255) tgcatgcGGTGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAg < 1:5685050/70‑1 (MQ=255) tgcatgcGGGGGCGGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAgg > 1:80829/1‑71 (MQ=255) tgcatgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAgg > 1:593047/1‑71 (MQ=255) tgcatgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAgg > 1:4770255/1‑71 (MQ=255) catgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCt > 1:4773053/1‑39 (MQ=255) catgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGtt > 1:1131947/1‑71 (MQ=255) catgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGta > 1:1015345/1‑70 (MQ=255) cGGGGTCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTg < 1:3284808/70‑1 (MQ=255) cGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTg < 1:1742415/70‑1 (MQ=255) cGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGt < 1:4345284/71‑1 (MQ=255) gggCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGt < 1:3067244/68‑1 (MQ=255) ttatgccagatgcGACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGTGCAGGttt < 1:3389087/59‑1 (MQ=18) agatgccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCg > 1:211415/1‑70 (MQ=255) gatgccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGa < 1:4228620/71‑1 (MQ=255) atgccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGa > 1:5637732/1‑70 (MQ=255) atgccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGAt < 1:5553128/71‑1 (MQ=255) gccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGAt > 1:1271032/1‑69 (MQ=255) ccagatgcGACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGTGCAGGt > 1:5415070/1‑54 (MQ=18) ccagatgcGACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGTGCAGGt > 1:4547068/1‑54 (MQ=18) agatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTg < 1:1369373/56‑1 (MQ=255) agatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTg < 1:1362622/56‑1 (MQ=255) gatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCg < 1:1307967/71‑1 (MQ=255) atgcGACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGTGCAgg < 1:3086673/49‑1 (MQ=18) tgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGtt < 1:1007146/71‑1 (MQ=255) tgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGtt < 1:5002663/71‑1 (MQ=255) tgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGtt > 1:1321382/1‑71 (MQ=255) tgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGt > 1:4442983/1‑70 (MQ=255) cGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGtt < 1:3756413/48‑1 (MQ=255) cGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTc < 1:2463774/70‑1 (MQ=255) gcgcGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAgg < 1:5446323/47‑1 (MQ=255) gcgcGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATa < 1:4034977/54‑1 (MQ=255) gcgcGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATa < 1:3914212/54‑1 (MQ=255) gcgcGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATa < 1:3854165/54‑1 (MQ=255) gcgcGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCa < 1:1038300/70‑1 (MQ=255) | AAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCA > Exported/2952795‑2952933 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |