| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 313,756 | A→T | 10.2% | D111E (GAT→GAA) | acrA ← | multidrug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 313,756 | 0 | A | T | 10.2% | 235.7 / 14.9 | 87 | D111E (GAT→GAA) | acrA | multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base A (32/46); new base T (4/5); total (36/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01 | |||||||||||
TACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTT > Exported/313686‑313826 | tACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGa < 1:655975/71‑1 (MQ=255) ccGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGAt < 1:3206658/70‑1 (MQ=255) ccGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGAt < 1:813290/70‑1 (MQ=255) ccGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGAt < 1:5485354/70‑1 (MQ=255) ccGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTCGCTGCAGCCTGGGCTTTCGCCAGAt < 1:3415134/70‑1 (MQ=255) ccGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTAGCTGCAGCCTGGGCTTTCGCCAGAt < 1:3421530/70‑1 (MQ=255) gAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCAc < 1:4211574/71‑1 (MQ=255) gCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGAt > 1:2975709/1‑66 (MQ=255) tttCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCg > 1:2412473/1‑71 (MQ=255) tCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGc < 1:5119620/70‑1 (MQ=255) tCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGc < 1:4201905/70‑1 (MQ=255) tCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa > 1:553005/1‑71 (MQ=255) tCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa > 1:3953699/1‑71 (MQ=255) tCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa > 1:1300063/1‑71 (MQ=255) tCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa > 1:1599952/1‑71 (MQ=255) cTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCAc > 1:5335662/1‑71 (MQ=255) tGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACt > 1:2341343/1‑71 (MQ=255) aCGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGt < 1:5612005/71‑1 (MQ=255) ttCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGa > 1:4672267/1‑48 (MQ=255) ttCACAGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGt > 1:4312247/1‑71 (MQ=255) tCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGa < 1:1158925/47‑1 (MQ=255) cACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCg > 1:4604195/1‑71 (MQ=255) aCCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCAc > 1:4115660/1‑58 (MQ=255) aCCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGc > 1:984623/1‑71 (MQ=255) cGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCt < 1:4053658/71‑1 (MQ=255) cGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCt < 1:3861191/71‑1 (MQ=255) tCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGa < 1:3068027/71‑1 (MQ=255) aaTTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGa > 1:1642031/1‑69 (MQ=255) aTTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAg < 1:1018523/71‑1 (MQ=255) tGCGCGATTTTGGCTGCAGCCTGGGCTTTCGCCAGATc < 1:4517387/38‑1 (MQ=255) tGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGa > 1:4796092/1‑66 (MQ=255) atatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAgg < 1:3929537/71‑1 (MQ=255) atatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAgg < 1:4099394/71‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCg < 1:3997587/47‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCg < 1:1788707/47‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGcc > 1:2795501/1‑56 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTg < 1:2777007/58‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTg < 1:1569277/58‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGc < 1:3847243/67‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAgg < 1:4106053/70‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAgg > 1:3301504/1‑70 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGa > 1:3319957/1‑71 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGa > 1:488604/1‑71 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGa > 1:2168727/1‑71 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGa > 1:5692641/1‑71 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCCCCTTTCGCACTGTCGTa > 1:2059740/1‑47 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTa > 1:5415865/1‑47 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTAt < 1:245898/48‑1 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATg < 1:2865650/49‑1 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGt < 1:972198/50‑1 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt > 1:1616252/1‑58 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt > 1:2566945/1‑58 (MQ=255) tGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTAt < 1:3406594/47‑1 (MQ=255) tGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTAt < 1:471907/47‑1 (MQ=255) tGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTAt < 1:2366677/47‑1 (MQ=255) ggCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCa < 1:2673492/70‑1 (MQ=255) ggCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCa < 1:2647068/70‑1 (MQ=255) gCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAg < 1:726102/58‑1 (MQ=255) cTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATa > 1:4880331/1‑69 (MQ=255) tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTa > 1:1303687/1‑35 (MQ=255) tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTa > 1:843830/1‑35 (MQ=255) tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATa > 1:2990771/1‑68 (MQ=255) gggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt < 1:5016708/45‑1 (MQ=255) gggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt < 1:4795491/45‑1 (MQ=255) gggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt < 1:5272139/45‑1 (MQ=255) gggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt < 1:2917242/45‑1 (MQ=255) ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagaga < 1:3043379/70‑1 (MQ=255) ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagag < 1:1591286/69‑1 (MQ=255) ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagag < 1:3598000/69‑1 (MQ=255) gCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagaga > 1:2471453/1‑69 (MQ=255) ttttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATcg < 1:4541670/55‑2 (MQ=255) cTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCaa < 1:2323984/57‑1 (MQ=255) cTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCaa < 1:1300814/57‑1 (MQ=255) tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag < 1:3241212/64‑1 (MQ=255) tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag < 1:2361173/64‑1 (MQ=255) tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag < 1:4010275/64‑1 (MQ=255) tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag < 1:5227791/64‑1 (MQ=255) ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGAt > 1:4953910/1‑56 (MQ=255) ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGAt > 1:3474709/1‑56 (MQ=255) ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggactg > 1:1668823/1‑68 (MQ=255) ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggact < 1:4523712/70‑3 (MQ=255) ttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag < 1:121238/63‑1 (MQ=255) tCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTgg < 1:1020961/59‑2 (MQ=255) tCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggactg < 1:762063/70‑4 (MQ=255) tCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggactg < 1:1499681/70‑4 (MQ=255) tCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGaca > 1:3964632/1‑71 (MQ=255) cGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga < 1:3554642/62‑1 (MQ=255) cGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGacac > 1:1295142/1‑71 (MQ=255) gCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTa > 1:23704/1‑59 (MQ=255) aGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGc > 1:4880651/1‑71 (MQ=255) aTCACCTTTCGCACTGTCGTATGTCGGCTGATAGGTCGCAGGATCAATTTGATAGAGAGAGACACCTGCt < 1:2381469/70‑1 (MQ=255) aTCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCg < 1:4235960/38‑1 (MQ=255) aTCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCg < 1:2793249/38‑1 (MQ=255) aTCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCtt > 1:499912/1‑71 (MQ=255) | TACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTT > Exported/313686‑313826 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |