BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	313,768	A→G	14.0%	S107S (AGT→AGC)	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	313,768	0	A	G	14.0%	240.0 / 26.0	100	S107S (AGT→AGC)	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand): ref base A (37/49); new base G (6/8); total (43/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 104 total aligned reads displayed.

TCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCG  >  Exported/313698‑313834

tCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa                                                                    >  1:1300063/1‑71 (MQ=255)

tCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa                                                                    >  1:3953699/1‑71 (MQ=255)

tCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa                                                                    >  1:1599952/1‑71 (MQ=255)

tCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCa                                                                    >  1:553005/1‑71 (MQ=255)

 cTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCAc                                                                   >  1:5335662/1‑71 (MQ=255)

  tGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACt                                                                  >  1:2341343/1‑71 (MQ=255)

       aCGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGt                                                             <  1:5612005/71‑1 (MQ=255)

           ttCACAGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGt                                                         >  1:4312247/1‑71 (MQ=255)

             cACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCg                                                       >  1:4604195/1‑71 (MQ=255)

              aCCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCAc                                                                   >  1:4115660/1‑58 (MQ=255)

                cGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCt                                                    <  1:4053658/71‑1 (MQ=255)

                cGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCt                                                    <  1:3861191/71‑1 (MQ=255)

                  tCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGa                                                  <  1:3068027/71‑1 (MQ=255)

                    aaTTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGa                                                  >  1:1642031/1‑69 (MQ=255)

                     aTTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAg                                               <  1:1018523/71‑1 (MQ=255)

                       tGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGa                                                  >  1:4796092/1‑66 (MQ=255)

                             atatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAgg                                       <  1:4099394/71‑1 (MQ=255)

                             atatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAgg                                       <  1:3929537/71‑1 (MQ=255)

                              tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCg                                                              <  1:1788707/47‑1 (MQ=255)

                              tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCg                                                              <  1:3997587/47‑1 (MQ=255)

                              tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGcc                                                     >  1:2795501/1‑56 (MQ=255)

                              tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTg                                                   <  1:1569277/58‑1 (MQ=255)

                              tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTg                                                   <  1:2777007/58‑1 (MQ=255)

                              tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGc                                          <  1:3847243/67‑1 (MQ=255)

                              tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAgg                                       <  1:4106053/70‑1 (MQ=255)

                              tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAgg                                       >  1:3301504/1‑70 (MQ=255)

                              tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGa                                      >  1:3319957/1‑71 (MQ=255)

                              tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGa                                      >  1:488604/1‑71 (MQ=255)

                              tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGa                                      >  1:5692641/1‑71 (MQ=255)

                              tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGa                                      >  1:2168727/1‑71 (MQ=255)

                                ttGGCTGCAGCCTGGGCTTTCGCCAGATCCCCTTTCGCACTGTCGTa                                                            >  1:2059740/1‑47 (MQ=255)

                                ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTa                                                            >  1:5415865/1‑47 (MQ=255)

                                ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATg                                                          <  1:2865650/49‑1 (MQ=255)

                                ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGt                                                         <  1:972198/50‑1 (MQ=255)

                                ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt                                                 >  1:1616252/1‑58 (MQ=255)

                                ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt                                                 >  1:2566945/1‑58 (MQ=255)

                                 tGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTAt                                                           <  1:3406594/47‑1 (MQ=255)

                                 tGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTAt                                                           <  1:2366677/47‑1 (MQ=255)

                                 tGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTAt                                                           <  1:471907/47‑1 (MQ=255)

                                  ggCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCa                                   <  1:2647068/70‑1 (MQ=255)

                                  ggCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCa                                   <  1:2673492/70‑1 (MQ=255)

                                         gCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAg                                        <  1:726102/58‑1 (MQ=255)

                                           cTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATa                           >  1:4880331/1‑69 (MQ=255)

                                            tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTa                                                            >  1:1303687/1‑35 (MQ=255)

                                            tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTa                                                            >  1:843830/1‑35 (MQ=255)

                                            tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATa                           >  1:2990771/1‑68 (MQ=255)

                                             gggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt                                                 <  1:5016708/45‑1 (MQ=255)

                                             gggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt                                                 <  1:5272139/45‑1 (MQ=255)

                                             gggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt                                                 <  1:2917242/45‑1 (MQ=255)

                                              ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagaga                       <  1:3043379/70‑1 (MQ=255)

                                              ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagag                        <  1:3598000/69‑1 (MQ=255)

                                              ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagag                        <  1:1591286/69‑1 (MQ=255)

                                               gCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagaga                       >  1:2471453/1‑69 (MQ=255)

                                                ttttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATcg                                   <  1:4541670/55‑2 (MQ=255)

                                                cTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCaa                                  <  1:1300814/57‑1 (MQ=255)

                                                cTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCaa                                  <  1:2323984/57‑1 (MQ=255)

                                                 tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag                          <  1:3241212/64‑1 (MQ=255)

                                                 tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag                          <  1:2361173/64‑1 (MQ=255)

                                                 tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag                          <  1:4010275/64‑1 (MQ=255)

                                                 tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag                          <  1:5227791/64‑1 (MQ=255)

                                                  ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGAt                                 >  1:3474709/1‑56 (MQ=255)

                                                  ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGAt                                 >  1:4953910/1‑56 (MQ=255)

                                                  ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggactg                  >  1:1668823/1‑68 (MQ=255)

                                                  ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggact                   <  1:4523712/70‑3 (MQ=255)

                                                  ttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag                          <  1:121238/63‑1 (MQ=255)

                                                   tCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTgg                             <  1:1020961/59‑2 (MQ=255)

                                                   tCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggactg                  <  1:762063/70‑4 (MQ=255)

                                                   tCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggactg                  <  1:1499681/70‑4 (MQ=255)

                                                   tCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGaca                 >  1:3964632/1‑71 (MQ=255)

                                                    cGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGacac                >  1:1295142/1‑71 (MQ=255)

                                                     gCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTa                           >  1:23704/1‑59 (MQ=255)

                                                        aGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGc            >  1:4880651/1‑71 (MQ=255)

                                                          aTCACCTTTCGCACTGTCGTATGTCGGCTGATAGGTCGCAGGATCAATTTGATAGAGAGAGACACCTGCt           <  1:2381469/70‑1 (MQ=255)

                                                          aTCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCg                                           <  1:2793249/38‑1 (MQ=255)

                                                          aTCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCg                                           <  1:4235960/38‑1 (MQ=255)

                                                          aTCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCtt          >  1:499912/1‑71 (MQ=255)

                                                           tCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc         <  1:3853621/71‑1 (MQ=255)

                                                           tCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc         <  1:5018371/71‑1 (MQ=255)

                                                            cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc         >  1:3260999/1‑70 (MQ=255)

                                                            cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc         >  1:3171989/1‑70 (MQ=255)

                                                            cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc         >  1:2200629/1‑70 (MQ=255)

                                                            cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc         >  1:67833/1‑70 (MQ=255)

                                                              ccTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTTATagagaga                     <  1:2828308/56‑1 (MQ=255)

                                                              ccTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagagaga                     <  1:4006488/56‑1 (MQ=255)

                                                               cTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagagaga                     <  1:304061/55‑1 (MQ=255)

                                                               cTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAgagagaga                   >  1:3375539/1‑57 (MQ=255)

                                                                tttCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacaggga                     <  1:2690401/54‑1 (MQ=255)

                                                                tttCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacaggga                     <  1:5173001/54‑1 (MQ=255)

                                                                tttCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacaggga                     <  1:367153/54‑1 (MQ=255)

                                                                tttCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga                         <  1:3441785/50‑1 (MQ=255)

                                                                tttCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga                         <  1:4040099/50‑1 (MQ=255)

                                                                 ttCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGc            >  1:5028477/1‑62 (MQ=255)

                                                                  tCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTgg                             >  1:4448477/1‑43 (MQ=255)

                                                                  tCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTgg                             >  1:3643475/1‑43 (MQ=255)

                                                                  tCGCACTGTCGTATGTCGCCTGATAGGTCGCAGTATCAATCTGATAGAGAGAGacac                >  1:512899/1‑57 (MQ=255)

                                                                  tCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCt                               >  1:4390208/1‑42 (MQ=255)

                                                                  tCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagag                        >  1:817059/1‑49 (MQ=255)

                                                                  tCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGacac                >  1:2912821/1‑57 (MQ=255)

                                                                   cGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCg  <  1:5474679/70‑1 (MQ=255)

                                                                    gCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCt                               <  1:2331018/40‑1 (MQ=255)

TCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCG  >  Exported/313698‑313834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`