| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 313,774 | G→A | 13.3% | Y105Y (TAC→TAT) | acrA ← | multidrug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 313,774 | 0 | G | A | 13.3% | 265.3 / 25.1 | 105 | Y105Y (TAC→TAT) | acrA | multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base G (35/56); new base A (6/8); total (41/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
Only 100 of 109 total aligned reads displayed.
ACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTT > Exported/313705‑313844 | aCGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGt < 1:5612005/71‑1 (MQ=255) cACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCg > 1:4604195/1‑71 (MQ=255) cGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCt < 1:4053658/71‑1 (MQ=255) tCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGa < 1:3068027/71‑1 (MQ=255) aaTTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGa > 1:1642031/1‑69 (MQ=255) aTTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAg < 1:1018523/71‑1 (MQ=255) tGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGa > 1:4796092/1‑66 (MQ=255) atatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAgg < 1:3929537/71‑1 (MQ=255) atatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAgg < 1:4099394/71‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCg < 1:3997587/47‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGcc > 1:2795501/1‑56 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTg < 1:1569277/58‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTg < 1:2777007/58‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGc < 1:3847243/67‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAgg < 1:4106053/70‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAgg > 1:3301504/1‑70 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGa > 1:5692641/1‑71 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGa > 1:2168727/1‑71 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCCCCTTTCGCACTGTCGTa > 1:2059740/1‑47 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTa > 1:5415865/1‑47 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATg < 1:2865650/49‑1 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGt < 1:972198/50‑1 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt > 1:1616252/1‑58 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt > 1:2566945/1‑58 (MQ=255) tGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTAt < 1:3406594/47‑1 (MQ=255) tGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTAt < 1:2366677/47‑1 (MQ=255) tGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTAt < 1:471907/47‑1 (MQ=255) ggCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCa < 1:2673492/70‑1 (MQ=255) ggCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCa < 1:2647068/70‑1 (MQ=255) gCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAg < 1:726102/58‑1 (MQ=255) cTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATa > 1:4880331/1‑69 (MQ=255) tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTa > 1:843830/1‑35 (MQ=255) tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTa > 1:1303687/1‑35 (MQ=255) tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATa > 1:2990771/1‑68 (MQ=255) gggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt < 1:4795491/45‑1 (MQ=255) gggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt < 1:5016708/45‑1 (MQ=255) gggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt < 1:5272139/45‑1 (MQ=255) ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagaga < 1:3043379/70‑1 (MQ=255) ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagag < 1:3598000/69‑1 (MQ=255) ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagag < 1:1591286/69‑1 (MQ=255) gCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagaga > 1:2471453/1‑69 (MQ=255) ttttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATcg < 1:4541670/55‑2 (MQ=255) cTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCaa < 1:2323984/57‑1 (MQ=255) cTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCaa < 1:1300814/57‑1 (MQ=255) tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag < 1:3241212/64‑1 (MQ=255) tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag < 1:5227791/64‑1 (MQ=255) tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag < 1:2361173/64‑1 (MQ=255) tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag < 1:4010275/64‑1 (MQ=255) ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGAt > 1:4953910/1‑56 (MQ=255) ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGAt > 1:3474709/1‑56 (MQ=255) ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggactg > 1:1668823/1‑68 (MQ=255) ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggact < 1:4523712/70‑3 (MQ=255) ttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag < 1:121238/63‑1 (MQ=255) tCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTgg < 1:1020961/59‑2 (MQ=255) tCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggactg < 1:1499681/70‑4 (MQ=255) tCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggactg < 1:762063/70‑4 (MQ=255) tCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGaca > 1:3964632/1‑71 (MQ=255) cGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga < 1:3554642/62‑1 (MQ=255) cGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGacac > 1:1295142/1‑71 (MQ=255) gCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTa > 1:23704/1‑59 (MQ=255) aGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGc > 1:4880651/1‑71 (MQ=255) aTCACCTTTCGCACTGTCGTATGTCGGCTGATAGGTCGCAGGATCAATTTGATAGAGAGAGACACCTGCt < 1:2381469/70‑1 (MQ=255) aTCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCg < 1:4235960/38‑1 (MQ=255) aTCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCg < 1:2793249/38‑1 (MQ=255) aTCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCtt > 1:499912/1‑71 (MQ=255) tCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc < 1:3853621/71‑1 (MQ=255) tCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc < 1:5018371/71‑1 (MQ=255) cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc > 1:3260999/1‑70 (MQ=255) cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc > 1:67833/1‑70 (MQ=255) cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc > 1:2200629/1‑70 (MQ=255) cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc > 1:3171989/1‑70 (MQ=255) ccTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTTATagagaga < 1:2828308/56‑1 (MQ=255) ccTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagagaga < 1:4006488/56‑1 (MQ=255) cTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagagaga < 1:304061/55‑1 (MQ=255) cTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAgagagaga > 1:3375539/1‑57 (MQ=255) tttCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacaggga < 1:2690401/54‑1 (MQ=255) tttCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacaggga < 1:5173001/54‑1 (MQ=255) tttCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacaggga < 1:367153/54‑1 (MQ=255) tttCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga < 1:4040099/50‑1 (MQ=255) tttCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga < 1:3441785/50‑1 (MQ=255) ttCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGc > 1:5028477/1‑62 (MQ=255) tCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTgg > 1:3643475/1‑43 (MQ=255) tCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTgg > 1:4448477/1‑43 (MQ=255) tCGCACTGTCGTATGTCGCCTGATAGGTCGCAGTATCAATCTGATAGAGAGAGacac > 1:512899/1‑57 (MQ=255) tCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCt > 1:4390208/1‑42 (MQ=255) tCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagag > 1:817059/1‑49 (MQ=255) tCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGacac > 1:2912821/1‑57 (MQ=255) gCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCt < 1:2331018/40‑1 (MQ=255) tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga < 1:2973631/42‑1 (MQ=255) tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga < 1:40323/42‑1 (MQ=255) tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga < 1:2967019/42‑1 (MQ=255) tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTc < 1:5569640/64‑1 (MQ=255) tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTc < 1:2271121/64‑1 (MQ=255) tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagaga > 1:5534865/1‑42 (MQ=255) tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCt < 1:5526433/65‑1 (MQ=255) tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCt < 1:1916086/65‑1 (MQ=255) tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACcttc < 1:3383050/71‑1 (MQ=255) tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACct < 1:3160888/69‑1 (MQ=255) gTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACcttctt > 1:5369406/1‑71 (MQ=255) gTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACcttct < 1:4555267/70‑1 (MQ=255) | ACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTT > Exported/313705‑313844 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |