| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 313,778 | G→T | 13.1% | T104K (ACA→AAA) ‡ | acrA ← | multidrug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 313,778 | 0 | G | T | 13.1% | 318.3 / 21.2 | 106 | T104K (ACA→AAA) ‡ | acrA | multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base G (33/59); new base T (6/8); total (39/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
TTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGA > Exported/313709‑313847 | ttCACAGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGt > 1:4312247/1‑71 (MQ=255) cACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCg > 1:4604195/1‑71 (MQ=255) cGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCt < 1:3861191/71‑1 (MQ=255) cGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCt < 1:4053658/71‑1 (MQ=255) tCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGa < 1:3068027/71‑1 (MQ=255) aaTTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGa > 1:1642031/1‑69 (MQ=255) aTTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAg < 1:1018523/71‑1 (MQ=255) tGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGa > 1:4796092/1‑66 (MQ=255) atatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAgg < 1:3929537/71‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGcc > 1:2795501/1‑56 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTg < 1:1569277/58‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTg < 1:2777007/58‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGc < 1:3847243/67‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAgg > 1:3301504/1‑70 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAgg < 1:4106053/70‑1 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGa > 1:3319957/1‑71 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGa > 1:488604/1‑71 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGa > 1:2168727/1‑71 (MQ=255) tatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGa > 1:5692641/1‑71 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATg < 1:2865650/49‑1 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGt < 1:972198/50‑1 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt > 1:1616252/1‑58 (MQ=255) ttGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt > 1:2566945/1‑58 (MQ=255) ggCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCa < 1:2673492/70‑1 (MQ=255) gCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAg < 1:726102/58‑1 (MQ=255) cTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATa > 1:4880331/1‑69 (MQ=255) tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATa > 1:2990771/1‑68 (MQ=255) gggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt < 1:5016708/45‑1 (MQ=255) gggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt < 1:5272139/45‑1 (MQ=255) gggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGAt < 1:2917242/45‑1 (MQ=255) ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagag < 1:3598000/69‑1 (MQ=255) ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagag < 1:1591286/69‑1 (MQ=255) gCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagaga > 1:2471453/1‑69 (MQ=255) ttttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATcg < 1:4541670/55‑2 (MQ=255) cTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCaa < 1:1300814/57‑1 (MQ=255) cTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCaa < 1:2323984/57‑1 (MQ=255) tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag < 1:2361173/64‑1 (MQ=255) tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag < 1:4010275/64‑1 (MQ=255) tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag < 1:3241212/64‑1 (MQ=255) tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag < 1:5227791/64‑1 (MQ=255) ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGAt > 1:4953910/1‑56 (MQ=255) ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGAt > 1:3474709/1‑56 (MQ=255) ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggactg > 1:1668823/1‑68 (MQ=255) ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggact < 1:4523712/70‑3 (MQ=255) ttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag < 1:121238/63‑1 (MQ=255) tCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTgg < 1:1020961/59‑2 (MQ=255) tCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggactg < 1:762063/70‑4 (MQ=255) tCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggactg < 1:1499681/70‑4 (MQ=255) tCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGaca > 1:3964632/1‑71 (MQ=255) cGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGacac > 1:1295142/1‑71 (MQ=255) gCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTa > 1:23704/1‑59 (MQ=255) aGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGc > 1:4880651/1‑71 (MQ=255) aTCACCTTTCGCACTGTCGTATGTCGGCTGATAGGTCGCAGGATCAATTTGATAGAGAGAGACACCTGCt < 1:2381469/70‑1 (MQ=255) aTCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCg < 1:4235960/38‑1 (MQ=255) aTCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCg < 1:2793249/38‑1 (MQ=255) aTCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCtt > 1:499912/1‑71 (MQ=255) tCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc < 1:5018371/71‑1 (MQ=255) tCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc < 1:3853621/71‑1 (MQ=255) cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc > 1:2200629/1‑70 (MQ=255) cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc > 1:3171989/1‑70 (MQ=255) cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc > 1:67833/1‑70 (MQ=255) cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc > 1:3260999/1‑70 (MQ=255) ccTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTTATagagaga < 1:2828308/56‑1 (MQ=255) ccTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagagaga < 1:4006488/56‑1 (MQ=255) cTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagagaga < 1:304061/55‑1 (MQ=255) cTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAgagagaga > 1:3375539/1‑57 (MQ=255) tttCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacaggga < 1:5173001/54‑1 (MQ=255) tttCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacaggga < 1:2690401/54‑1 (MQ=255) tttCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacaggga < 1:367153/54‑1 (MQ=255) tttCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga < 1:3441785/50‑1 (MQ=255) tttCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga < 1:4040099/50‑1 (MQ=255) ttCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGc > 1:5028477/1‑62 (MQ=255) tCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTgg > 1:4448477/1‑43 (MQ=255) tCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTgg > 1:3643475/1‑43 (MQ=255) tCGCACTGTCGTATGTCGCCTGATAGGTCGCAGTATCAATCTGATAGAGAGAGacac > 1:512899/1‑57 (MQ=255) tCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCt > 1:4390208/1‑42 (MQ=255) tCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagag > 1:817059/1‑49 (MQ=255) tCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGacac > 1:2912821/1‑57 (MQ=255) cGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCg < 1:5474679/70‑1 (MQ=255) gCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCt < 1:2331018/40‑1 (MQ=255) tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga < 1:2967019/42‑1 (MQ=255) tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga < 1:2973631/42‑1 (MQ=255) tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga < 1:40323/42‑1 (MQ=255) tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTc < 1:5569640/64‑1 (MQ=255) tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTc < 1:2271121/64‑1 (MQ=255) tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagaga > 1:5534865/1‑42 (MQ=255) tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCt < 1:5526433/65‑1 (MQ=255) tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACcttc < 1:3383050/71‑1 (MQ=255) tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACct < 1:3160888/69‑1 (MQ=255) gTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACcttct < 1:4555267/70‑1 (MQ=255) aTGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCttt > 1:2100569/1‑70 (MQ=255) tGTCGCCTGATAGGTCGCAGGGTCAATCGGATaga < 1:741158/35‑1 (MQ=38) tGTCGCCTGATAGGTCGCAGGATCAATCTGATaga < 1:2251736/35‑1 (MQ=255) tGTCGCCTGATAGGTCGCAGGATCAATCTGATaga < 1:4241240/35‑1 (MQ=255) tGTCGCCTGATAGGTCGCAGGATCAATCTGATaga < 1:3258420/35‑1 (MQ=255) tGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGATAGACACCTGCTTc < 1:680537/51‑1 (MQ=255) tGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc < 1:4658673/51‑1 (MQ=255) tGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGa < 1:1209712/71‑1 (MQ=255) tGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGa < 1:1669876/71‑1 (MQ=255) gTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGa > 1:4420989/1‑70 (MQ=255) | TTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGA > Exported/313709‑313847 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |