Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 132,220 G→T 24.1% R171R (CGG→CGT yadD → predicted transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported132,2200GT24.1% 214.1 / 45.9 99R171R (CGG→CGTyadDpredicted transposase
Reads supporting (aligned to +/- strand):  ref base G (33/42);  new base T (15/9);  total (48/51)
Fisher's exact test for biased strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 109 total aligned reads displayed.

TCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACT  >  Exported/132150‑132288
                                                                      |                                                                    
tCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCTTGCAACATcggcgg                                                                      <  1:3914110/71‑1 (MQ=255)
  tataACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGAt                                                                    >  1:4600577/1‑71 (MQ=255)
   ataACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGAtt                                                                   >  1:4143045/1‑71 (MQ=255)
      aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGc                                                                 <  1:3522708/70‑1 (MQ=255)
      aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGc                                                                 <  1:2161053/70‑1 (MQ=255)
       cAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa                                                               <  1:1986915/71‑1 (MQ=255)
            cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                                          <  1:1800412/71‑1 (MQ=255)
             tttCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                                          >  1:648428/1‑70 (MQ=255)
              ttCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCTACATCGGCGGATTGCGATTCTCg                                                         >  1:2903375/1‑70 (MQ=255)
               tCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                       >  1:5084483/1‑71 (MQ=255)
                cccGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                         <  1:4883306/68‑1 (MQ=255)
                 ccGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:4287679/70‑1 (MQ=255)
                  cGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:3269773/69‑1 (MQ=255)
                    cTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:5320433/1‑69 (MQ=255)
                    cTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:1291540/1‑69 (MQ=255)
                       gtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCa                                               <  1:5028866/71‑1 (MQ=255)
                          gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                            <  1:2104096/71‑1 (MQ=255)
                          gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                            <  1:3186170/71‑1 (MQ=255)
                          gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                             <  1:5527922/70‑1 (MQ=255)
                           atatCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTAtag                                                                  <  1:3532925/48‑3 (MQ=255)
                           atatCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                            <  1:2659004/70‑1 (MQ=255)
                              taaCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:3366443/3‑59 (MQ=255)
                              tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGct                                                                >  1:1639548/1‑46 (MQ=255)
                              tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:5194850/1‑59 (MQ=255)
                              tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:1715133/1‑59 (MQ=255)
                              tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:3654363/1‑59 (MQ=255)
                               cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                                       >  1:4456591/1‑71 (MQ=255)
                               cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                        >  1:5139991/1‑70 (MQ=255)
                               cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                        >  1:3151006/1‑70 (MQ=255)
                               cACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                       >  1:137935/1‑71 (MQ=255)
                                aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                                       <  1:1682564/70‑1 (MQ=255)
                                aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                      <  1:5411175/71‑1 (MQ=255)
                                   aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCg                                   <  1:4109234/71‑1 (MQ=255)
                                    tCACAGCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                         <  1:793773/48‑1 (MQ=255)
                                    tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                                                  >  1:2371299/1‑39 (MQ=255)
                                    tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                                                  >  1:1589802/1‑39 (MQ=255)
                                    tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                                                  >  1:3622995/1‑39 (MQ=255)
                                    tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                                                  >  1:2733784/1‑39 (MQ=255)
                                    tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                                                  >  1:3342140/1‑39 (MQ=255)
                                    tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    <  1:1364574/53‑1 (MQ=255)
                                    tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                  >  1:1724271/1‑71 (MQ=255)
                                    tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                  <  1:2811198/71‑1 (MQ=255)
                                    tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                  <  1:1990572/71‑1 (MQ=255)
                                     cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                       >  1:1119891/1‑49 (MQ=255)
                                     cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                       >  1:1469273/1‑49 (MQ=255)
                                     cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                       >  1:2427586/1‑49 (MQ=255)
                                     cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                       >  1:283599/1‑49 (MQ=255)
                                     cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGa                                                        >  1:824333/1‑48 (MQ=255)
                                      acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                 <  1:5225852/70‑1 (MQ=255)
                                      acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                 <  1:1456262/70‑1 (MQ=255)
                                        acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                            <  1:1591358/57‑1 (MQ=255)
                                        acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                            <  1:4922131/57‑1 (MQ=255)
                                          cGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc                            >  1:5603944/1‑71 (MQ=255)
                                          cGGATGACGAAATCATGCAACATAGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcg                             >  1:4314735/1‑70 (MQ=255)
                                           ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                         <  1:5499548/57‑1 (MQ=255)
                                           ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                         <  1:4411448/57‑1 (MQ=255)
                                             aTGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaat                                            <  1:1765041/52‑2 (MQ=255)
                                              tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:1419189/41‑1 (MQ=255)
                                              tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:3950972/41‑1 (MQ=255)
                                              tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:2405323/41‑1 (MQ=255)
                                              tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:5469016/41‑1 (MQ=255)
                                              tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                  <  1:5480886/61‑1 (MQ=255)
                                              tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACt                        <  1:5105414/71‑1 (MQ=255)
                                                aCGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACtt                       >  1:4311932/1‑70 (MQ=255)
                                                   agaTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtc                   >  1:5264174/3‑70 (MQ=255)
                                                    aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                  <  1:4346016/71‑1 (MQ=255)
                                                     aTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACcgcg                           <  1:1981471/61‑1 (MQ=255)
                                                     aTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtcgg                 <  1:3144933/71‑4 (MQ=255)
                                                      tCATGCAACATCGGCGTATAGCTCTGCTTGAACTGATTCAAAAGCATATTCGCGACCGCGat                         <  1:437409/62‑2 (MQ=255)
                                                      tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                         <  1:5381492/62‑2 (MQ=255)
                                                      tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                         <  1:3389478/62‑2 (MQ=255)
                                                      tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGAttt                       <  1:4614380/64‑1 (MQ=255)
                                                      tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtcgg                 <  1:4086207/70‑4 (MQ=255)
                                                      tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                           <  1:5304759/44‑1 (MQ=255)
                                                      tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                           <  1:2386059/44‑1 (MQ=255)
                                                      tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                           <  1:3964826/44‑1 (MQ=255)
                                                       cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                                       >  1:1096838/1‑47 (MQ=255)
                                                       cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                        >  1:3949494/1‑46 (MQ=255)
                                                       cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                        >  1:3643736/1‑46 (MQ=255)
                                                       cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                        >  1:3007904/1‑46 (MQ=255)
                                                       cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                         >  1:2371657/1‑60 (MQ=255)
                                                       cATGCAACATAGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                         >  1:3248330/1‑60 (MQ=255)
                                                        aTGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                         >  1:176267/1‑44 (MQ=255)
                                                         tGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                             <  1:202621/39‑1 (MQ=255)
                                                          gCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGcga                                >  1:2947347/1‑49 (MQ=255)
                                                           cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt           <  1:4014833/71‑1 (MQ=255)
                                                           cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt           >  1:121453/1‑71 (MQ=255)
                                                           cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt           >  1:1755295/1‑71 (MQ=255)
                                                            aaCATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                      >  1:2550432/1‑59 (MQ=255)
                                                            aaCATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                      >  1:2398642/1‑59 (MQ=255)
                                                             aCATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc                            <  1:825848/52‑1 (MQ=255)
                                                               aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                     >  1:4756399/1‑57 (MQ=255)
                                                               aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg       >  1:3152257/1‑71 (MQ=255)
                                                                 cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                    >  1:4727016/1‑56 (MQ=255)
                                                                 cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                    >  1:5388259/1‑56 (MQ=255)
                                                                 cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                    >  1:4733468/1‑56 (MQ=255)
                                                                  ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa    >  1:549698/1‑71 (MQ=255)
                                                                  ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa    <  1:1932043/71‑1 (MQ=255)
                                                                  ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa    <  1:1726524/71‑1 (MQ=255)
                                                                     ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt  <  1:1854585/70‑1 (MQ=255)
                                                                      |                                                                    
TCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACT  >  Exported/132150‑132288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: