BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	313,807	A→G	9.3%	Y94Y (TAT→TAC)	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	313,807	0	A	G	9.3%	261.4 / 13.1	96	Y94Y (TAT→TAC)	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand): ref base A (37/50); new base G (2/7); total (39/57)
Fisher's exact test for biased strand distribution p-value = 3.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

CTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTT  >  Exported/313741‑313877

cTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATa                                                                      >  1:4880331/1‑69 (MQ=255)

 tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATa                                                                      >  1:2990771/1‑68 (MQ=255)

   ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagaga                                                                  <  1:3043379/70‑1 (MQ=255)

   ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagag                                                                   <  1:1591286/69‑1 (MQ=255)

   ggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagag                                                                   <  1:3598000/69‑1 (MQ=255)

    gCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagaga                                                                  >  1:2471453/1‑69 (MQ=255)

      tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag                                                                     <  1:5227791/64‑1 (MQ=255)

      tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag                                                                     <  1:4010275/64‑1 (MQ=255)

      tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag                                                                     <  1:3241212/64‑1 (MQ=255)

      tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag                                                                     <  1:2361173/64‑1 (MQ=255)

       ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggactg                                                             >  1:1668823/1‑68 (MQ=255)

       ttCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggact                                                              <  1:4523712/70‑3 (MQ=255)

       ttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATag                                                                     <  1:121238/63‑1 (MQ=255)

        tCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggactg                                                             <  1:762063/70‑4 (MQ=255)

        tCGCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacagggactg                                                             <  1:1499681/70‑4 (MQ=255)

        tCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGaca                                                            >  1:3964632/1‑71 (MQ=255)

         cGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga                                                                    <  1:3554642/62‑1 (MQ=255)

         cGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGacac                                                           >  1:1295142/1‑71 (MQ=255)

          gCCAGTTCGCCTTTCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTa                                                                      >  1:23704/1‑59 (MQ=255)

             aGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGc                                                       >  1:4880651/1‑71 (MQ=255)

               aTCACCTTTCGCACTGTCGTATGTCGGCTGATAGGTCGCAGGATCAATTTGATAGAGAGAGACACCTGCt                                                      <  1:2381469/70‑1 (MQ=255)

               aTCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCtt                                                     >  1:499912/1‑71 (MQ=255)

                tCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc                                                    <  1:3853621/71‑1 (MQ=255)

                tCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc                                                    <  1:5018371/71‑1 (MQ=255)

                 cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc                                                    >  1:3260999/1‑70 (MQ=255)

                 cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc                                                    >  1:67833/1‑70 (MQ=255)

                 cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc                                                    >  1:3171989/1‑70 (MQ=255)

                 cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc                                                    >  1:2200629/1‑70 (MQ=255)

                   ccTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTTATagagaga                                                                <  1:2828308/56‑1 (MQ=255)

                   ccTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagagaga                                                                <  1:4006488/56‑1 (MQ=255)

                    cTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagagaga                                                                <  1:304061/55‑1 (MQ=255)

                    cTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAgagagaga                                                              >  1:3375539/1‑57 (MQ=255)

                     tttCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacaggga                                                                <  1:367153/54‑1 (MQ=255)

                     tttCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacaggga                                                                <  1:2690401/54‑1 (MQ=255)

                     tttCGCGCTGTCATAATTTGCCTGATAGGTCGCGGGATCGATCTGGTacaggga                                                                <  1:5173001/54‑1 (MQ=255)

                     tttCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga                                                                    <  1:4040099/50‑1 (MQ=255)

                     tttCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga                                                                    <  1:3441785/50‑1 (MQ=255)

                      ttCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGc                                                       >  1:5028477/1‑62 (MQ=255)

                       tCGCACTGTCGTATGTCGCCTGATAGGTCGCAGTATCAATCTGATAGAGAGAGacac                                                           >  1:512899/1‑57 (MQ=255)

                       tCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagag                                                                   >  1:817059/1‑49 (MQ=255)

                       tCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGacac                                                           >  1:2912821/1‑57 (MQ=255)

                        cGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCg                                             <  1:5474679/70‑1 (MQ=255)

                             tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga                                                                    <  1:2973631/42‑1 (MQ=255)

                             tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga                                                                    <  1:2967019/42‑1 (MQ=255)

                             tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATaga                                                                    <  1:40323/42‑1 (MQ=255)

                             tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTc                                              <  1:5569640/64‑1 (MQ=255)

                             tGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTc                                              <  1:2271121/64‑1 (MQ=255)

                               tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATagaga                                                                  >  1:5534865/1‑42 (MQ=255)

                               tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCt                                           <  1:5526433/65‑1 (MQ=255)

                               tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCt                                           <  1:1916086/65‑1 (MQ=255)

                               tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACcttc                                     <  1:3383050/71‑1 (MQ=255)

                               tCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACct                                       <  1:3160888/69‑1 (MQ=255)

                                 gTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACcttctt                                   >  1:5369406/1‑71 (MQ=255)

                                 gTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACcttct                                    <  1:4555267/70‑1 (MQ=255)

                                   aTGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCttt                                  >  1:2100569/1‑70 (MQ=255)

                                    tGTCGCCTGATAGGTCGCAGGGTCAATCGGATaga                                                                    <  1:741158/35‑1 (MQ=38)

                                    tGTCGCCTGATAGGTCGCAGGATCAATCTGATaga                                                                    <  1:2251736/35‑1 (MQ=255)

                                    tGTCGCCTGATAGGTCGCAGGATCAATCTGATaga                                                                    <  1:3258420/35‑1 (MQ=255)

                                    tGTCGCCTGATAGGTCGCAGGATCAATCTGATaga                                                                    <  1:4241240/35‑1 (MQ=255)

                                    tGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGATAGACACCTGCTTc                                                    <  1:680537/51‑1 (MQ=255)

                                    tGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc                                                    <  1:4658673/51‑1 (MQ=255)

                                    tGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGa                                <  1:1209712/71‑1 (MQ=255)

                                    tGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGa                                <  1:1669876/71‑1 (MQ=255)

                                     gTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGa                                >  1:4420989/1‑70 (MQ=255)

                                      tCGCCTGATAGGTCGCAGGATCAATCTGATagaga                                                                  <  1:529635/35‑1 (MQ=255)

                                      tCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc                                                    <  1:1296612/49‑1 (MQ=255)

                                      tCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTc                                                    <  1:3872741/49‑1 (MQ=255)

                                      tCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTg                                 >  1:4620502/1‑68 (MQ=255)

                                      tCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTg                                 >  1:4657609/1‑68 (MQ=255)

                                      tCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGaaa                              >  1:4658335/1‑71 (MQ=255)

                                      tCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGaaa                              >  1:4796847/1‑71 (MQ=255)

                                      tCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGaaa                              >  1:4803369/1‑71 (MQ=255)

                                      tCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGaaa                              >  1:2910723/1‑71 (MQ=255)

                                      tCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGaaa                              >  1:884865/1‑71 (MQ=255)

                                        gCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAAt                             >  1:4009006/1‑70 (MQ=255)

                                        gCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAAt                             <  1:1600183/70‑1 (MQ=255)

                                        gCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAAt                             <  1:5370912/70‑1 (MQ=255)

                                          cTGATAGGTCGCGGGATCGATCTGGTACAGGGACTGGCCTGCTTGCACATCGCTGCCTTCAGTGAAAttgc                          <  1:1309565/71‑3 (MQ=255)

                                          cTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTAc                          <  1:2489528/71‑1 (MQ=255)

                                          cTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTAc                          <  1:2847420/71‑1 (MQ=255)

                                            gATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGa                                <  1:1383000/63‑1 (MQ=255)

                                            gATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGc                        >  1:5075756/1‑71 (MQ=255)

                                            gATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGc                        >  1:1979692/1‑71 (MQ=255)

                                                    gCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGAt                <  1:772659/71‑1 (MQ=255)

                                                    gCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGAt                <  1:261144/71‑1 (MQ=255)

                                                      aGGATCAATCTGAAAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGTTTCAGGATaa              <  1:2256437/71‑1 (MQ=255)

                                                        gATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGa                                <  1:4522382/51‑1 (MQ=255)

                                                         aTCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACcttctt                                   <  1:4295037/47‑1 (MQ=255)

                                                         aTCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGa                                <  1:3180622/50‑1 (MQ=255)

                                                          tCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAAt                             >  1:4254792/1‑52 (MQ=255)

                                                          tCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAAt                             >  1:5443208/1‑52 (MQ=255)

                                                          tCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATccc          >  1:3630095/1‑71 (MQ=255)

                                                          tCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATcc           >  1:88970/1‑70 (MQ=255)

                                                           cAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCa                    >  1:637433/1‑60 (MQ=255)

                                                                tGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAAc    >  1:5186021/1‑71 (MQ=255)

                                                                  aTAGAGAGAGACACCTGCTTCGATGTCGCTACcttc                                     <  1:4130064/36‑1 (MQ=255)

                                                                  aTAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGaaa                              >  1:2656605/1‑43 (MQ=255)

                                                                  aTAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTa      <  1:472724/67‑1 (MQ=255)

                                                                  aTAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACtt  <  1:4302614/71‑1 (MQ=255)

                                                                  aTAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACt   <  1:4365912/70‑1 (MQ=255)

CTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTT  >  Exported/313741‑313877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`