Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	313,907	C→T	19.4%	S61N (AGT→AAT)	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	313,907	0	C	T	19.4%	149.7 / 27.9	72	S61N (AGT→AAT)	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base C (25/33);  new base T (12/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 6.18e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

CCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGG  >  Exported/313838‑313976                                                                      |                                                                      ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt                                                                      >  1:1876190/1‑71 (MQ=255) ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt                                                                      >  1:1869642/1‑71 (MQ=255)     cttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTg                                                                  >  1:3984667/1‑71 (MQ=255)      tttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc                                                                 >  1:2760457/1‑71 (MQ=255)      tttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc                                                                 >  1:513687/1‑71 (MQ=255)       gtGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCTGCTATACGATAAGCATTGGTGCg                                                                <  1:2343094/70‑1 (MQ=255)        tGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCgg                                                               >  1:1375392/1‑70 (MQ=255)         gAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGa                                                               <  1:5551224/70‑1 (MQ=255)          aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGt                                                                   >  1:1981234/1‑65 (MQ=255)          aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                                                                  >  1:3001702/1‑66 (MQ=255)          aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                                                                  >  1:1572776/1‑66 (MQ=255)          aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                                                                  >  1:536100/1‑66 (MQ=255)          aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCggc                                                              >  1:4205515/1‑68 (MQ=255)          aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCGGcc                                                             >  1:2215143/1‑71 (MQ=255)          aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCGGcc                                                             <  1:4455268/71‑1 (MQ=255)          aaaTTACTCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAc                                                              <  1:5693475/70‑1 (MQ=255)          aaaTTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAcc                                                             >  1:2856905/1‑71 (MQ=255)            aTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                                                     >  1:5356662/1‑61 (MQ=255)            aTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                                                     >  1:5135467/1‑61 (MQ=255)            aTTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCg                                                           <  1:2536126/71‑1 (MQ=255)             ttACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg                                                          <  1:2171877/71‑1 (MQ=255)             ttACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg                                                          <  1:4605918/71‑1 (MQ=255)               aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGa                                                         <  1:2308429/70‑1 (MQ=255)                 gCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGc                                                      >  1:139169/1‑71 (MQ=255)                  cTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCt                                                     <  1:1138572/71‑1 (MQ=255)                    tCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTc                                                    >  1:4285273/1‑70 (MQ=255)                     cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg                                                  >  1:3156673/1‑71 (MQ=255)                     cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg                                                  >  1:723581/1‑71 (MQ=255)                     cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCg                                                   <  1:5147570/70‑1 (MQ=255)                      aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                                                                     >  1:887874/1‑51 (MQ=255)                      aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                                                                     >  1:2580969/1‑51 (MQ=255)                      aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                                                                     >  1:1081018/1‑51 (MQ=255)                       ggATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGtt                                                >  1:1133632/1‑71 (MQ=255)                        gATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtg                                               <  1:663073/71‑1 (MQ=255)                          tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                             <  1:4781529/71‑1 (MQ=255)                          tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                             <  1:2803890/71‑1 (MQ=255)                          tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                             <  1:157456/71‑1 (MQ=255)                          tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                             <  1:4601654/71‑1 (MQ=255)                            aTCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGa                                            <  1:28518/70‑1 (MQ=255)                            aTCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                                                     >  1:180262/1‑45 (MQ=255)                            aTCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                                                     >  1:3751927/1‑45 (MQ=255)                            aTCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                                                     >  1:1602933/1‑45 (MQ=255)                               ccGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTg                                        >  1:4630114/1‑71 (MQ=255)                                 gCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCa                                      <  1:5687358/71‑1 (MQ=255)                                  cTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag                                     <  1:4744225/71‑1 (MQ=255)                                  cTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag                                     <  1:2961360/71‑1 (MQ=255)                                  cTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag                                     <  1:2953582/71‑1 (MQ=255)                                   tAACTTGAGGAGGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                             <  1:4120954/62‑1 (MQ=255)                                   tAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGtt                                                >  1:464566/1‑59 (MQ=255)                                   tAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                             <  1:1502636/62‑1 (MQ=255)                                   tAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                             <  1:2541091/62‑1 (MQ=255)                                    aaCTTGAGGACGAACTTCTGCGGTCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCagag                                   >  1:5040791/1‑71 (MQ=255)                                    aaCTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                             <  1:2107542/61‑1 (MQ=255)                                      cTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGAt                                           >  1:4652778/1‑61 (MQ=255)                                       ttGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                             <  1:1048885/58‑1 (MQ=255)                                         gAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGAt                                           >  1:4345115/1‑58 (MQ=255)                                            gACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag                                     <  1:2579096/61‑1 (MQ=255)                                                     cTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGt                   <  1:3090748/70‑1 (MQ=255)                                                       gCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGtttt                         <  1:1925149/62‑1 (MQ=255)                                                        cGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTtac                <  1:4439050/70‑1 (MQ=255)                                                             cGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGcc          <  1:4939065/71‑1 (MQ=255)                                                             cGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGcc          <  1:3288105/71‑1 (MQ=255)                                                                  ggCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGtt                           <  1:108045/49‑1 (MQ=255)                                                                   gCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTc                               <  1:3546895/44‑1 (MQ=255)                                                                   gCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcggc    <  1:1012473/71‑1 (MQ=255)                                                                   gCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcggc    <  1:1390951/71‑1 (MQ=255)                                                                     aCTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCaa        >  1:3745896/1‑65 (MQ=255)                                                                     aCTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcggcgg  >  1:3806753/1‑71 (MQ=255)                                                                     aCTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcggcgg  >  1:3998447/1‑71 (MQ=255)                                                                     aCTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcggcgg  >  1:4151366/1‑71 (MQ=255)                                                                     aCTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcggcgg  >  1:4838113/1‑71 (MQ=255)                                                                     aCTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcggcgg  >  1:4494780/1‑71 (MQ=255)                                                                      |                                                                      CCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGG  >  Exported/313838‑313976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑