| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 349,960 | C→T | 62.5% | P155L (CCC→CTC) | hyi → | hydroxypyruvate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 349,960 | 0 | C | T | 62.5% | 54.0 / 69.1 | 72 | P155L (CCC→CTC) | hyi | hydroxypyruvate isomerase |
| Reads supporting (aligned to +/- strand): ref base C (12/15); new base T (17/28); total (29/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAG > Exported/349891‑350024 | tGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATcc < 1:3084683/70‑1 (MQ=255) gatgaAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCg > 1:2128141/1‑71 (MQ=255) atgaAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGAAATTCCTGGTTTCCATCCCACCgg < 1:4434988/71‑1 (MQ=255) gaagaTATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCg > 1:5323918/1‑71 (MQ=255) aagaTATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCgg < 1:4510585/71‑1 (MQ=255) aagaTATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCgg < 1:1837973/71‑1 (MQ=255) agaTATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGc < 1:410145/71‑1 (MQ=255) agaTATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGc < 1:2776779/71‑1 (MQ=255) gaTATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCg > 1:4462012/1‑68 (MQ=255) gaTATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCg > 1:4171305/1‑68 (MQ=255) gaTATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCg > 1:5428596/1‑68 (MQ=255) gaTATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCa < 1:3250166/71‑1 (MQ=255) ttattaCTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCt > 1:2108580/1‑71 (MQ=255) ttattaCTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCAc > 1:4541229/1‑53 (MQ=255) ttattaCTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCAc > 1:3797272/1‑53 (MQ=255) ttattaCTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCAc > 1:106318/1‑53 (MQ=255) ttattaCTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCAc > 1:647987/1‑53 (MQ=255) ttattaCTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCAc > 1:1276846/1‑53 (MQ=255) ttattaCTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGgcgc < 1:2635219/70‑1 (MQ=255) tattaCTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCt < 1:3965963/70‑1 (MQ=255) ttaCTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGa < 1:153782/70‑1 (MQ=255) ttGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCgg < 1:238731/53‑1 (MQ=255) gAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAattgattg < 1:5645733/70‑1 (MQ=255) gAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAattgattg < 1:1561460/70‑1 (MQ=255) aaCCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATtgattgat < 1:1674394/71‑1 (MQ=255) aCCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGa < 1:2145037/43‑1 (MQ=255) aCCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGa < 1:3210566/43‑1 (MQ=255) aCCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATtgatg < 1:1315247/71‑1 (MQ=255) cTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGaaa < 1:3873809/59‑1 (MQ=255) tATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAat < 1:2055325/59‑1 (MQ=255) tATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAat < 1:4433608/59‑1 (MQ=255) aTTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgt > 1:1356333/1‑71 (MQ=255) ttAACCATTTTGATATTCCTGGTTTCCATCTCACCgg < 1:5573606/37‑1 (MQ=255) ttAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCgg < 1:444620/44‑1 (MQ=255) ttAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgtt > 1:3187932/1‑71 (MQ=255) aaCCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGc < 1:424486/43‑1 (MQ=255) aaCCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGc < 1:1291537/43‑1 (MQ=255) aaCCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGc < 1:1310847/43‑1 (MQ=255) aaCCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCa > 1:4979480/1‑44 (MQ=255) aaCCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATtgatga > 1:356831/1‑65 (MQ=255) ccATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATtgatg < 1:2255553/62‑1 (MQ=255) cATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCt < 1:2655740/47‑1 (MQ=255) aTTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGaaa > 1:2365573/1‑50 (MQ=255) ttttGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGgcg > 1:1461813/1‑43 (MQ=255) ttttGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGa < 1:4691159/47‑1 (MQ=255) ttttGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGa < 1:417159/47‑1 (MQ=255) ttttGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATtgattgat < 1:1070845/58‑1 (MQ=255) ttttGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgttggt < 1:5512542/67‑1 (MQ=255) ttttGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATtgatga < 1:3733347/60‑1 (MQ=255) tttGATATTCCTGGTTTCCATCTCACCGGAACTCGGCa < 1:819388/38‑1 (MQ=255) tttGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTtgctg > 1:2654870/1‑71 (MQ=255) tttGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAat > 1:1777536/1‑49 (MQ=255) gATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGatgttggtt < 1:1022585/61‑4 (MQ=255) gATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATAGatgttggtt < 1:4770937/61‑4 (MQ=255) atatTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAAt < 1:5195275/71‑1 (MQ=255) atatTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAAt < 1:97888/71‑1 (MQ=255) atatTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAAt < 1:4522861/71‑1 (MQ=255) tatTCCTGGTTTCCATCTCACCGGAACTCGGCAGgcgc > 1:3183470/1‑38 (MQ=255) tatTCCTGGTTTCCATCTCACCGGAACTCGGCAGgcgc > 1:1125974/1‑38 (MQ=255) ttCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAattg < 1:1283683/44‑1 (MQ=255) ccTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGa < 1:2398437/70‑1 (MQ=255) ccTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATtgattgat > 1:2635514/1‑48 (MQ=255) ccTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATtgattgat > 1:1827473/1‑48 (MQ=255) ccTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGa < 1:3029184/70‑1 (MQ=255) ggTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGaaaa > 1:2277117/1‑70 (MQ=255) ggTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAAt > 1:500080/1‑71 (MQ=255) gTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAAt < 1:5138495/70‑1 (MQ=255) tCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCa < 1:2532224/70‑1 (MQ=255) ccATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAg > 1:1544460/1‑70 (MQ=255) ccATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgttg > 1:4887503/1‑47 (MQ=255) ccATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgttg > 1:772974/1‑47 (MQ=255) aTCCCACCGGAACTCGGCAGGCGCTGAAATtgattgat < 1:5487573/38‑1 (MQ=255) tCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgt > 1:1965698/1‑42 (MQ=255) ccACCGGAACTCGGCAGGCGCTGAAATTGATtgatg > 1:5064456/1‑36 (MQ=255) ccACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGaa < 1:2259166/58‑1 (MQ=255) | TGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAG > Exported/349891‑350024 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |