Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 364,249 G→T 9.2% intergenic (‑121/+107) intD ← / ← entD predicted integrase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported364,2490GT 9.2% 269.4 / 8.8 86intergenic (‑121/+107)intD/entDpredicted integrase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
Reads supporting (aligned to +/- strand):  ref base G (26/52);  new base T (3/5);  total (29/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCA  >  Exported/364181‑364318
                                                                    |                                                                     
cGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCCACCCTTAAGTTAGCGCTTATGGGAGt                                                                      >  1:4246513/1‑70 (MQ=255)
cGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGt                                                                      >  1:2911336/1‑70 (MQ=255)
 gTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTaa                                                                    <  1:2843652/71‑1 (MQ=255)
  tCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTa                                                                     <  1:4375294/69‑1 (MQ=255)
  tCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAAt                                                                   <  1:1395355/71‑1 (MQ=255)
  tCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAAt                                                                   <  1:3955489/71‑1 (MQ=255)
     tAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATccc                                                                >  1:562200/1‑71 (MQ=255)
        cGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCg                                                              >  1:2286732/1‑70 (MQ=38)
         gAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCa                                                            <  1:2409012/71‑1 (MQ=255)
          aGGCACCGAGGCGTCGCATTATTCAGATGGTTAAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGccg                                                           >  1:836154/1‑69 (MQ=25)
           ggCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCata                                                          >  1:2950834/1‑71 (MQ=255)
            gCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCatat                                                         >  1:4952906/1‑71 (MQ=255)
               ccGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCg                                                      <  1:2431916/71‑1 (MQ=255)
               ccGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCg                                                      <  1:5560619/71‑1 (MQ=255)
                 gAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCg                                                      >  1:1810174/1‑69 (MQ=255)
                    gCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTgt                                                 <  1:2793360/71‑1 (MQ=255)
                    gCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTgt                                                 <  1:3335641/71‑1 (MQ=255)
                    gCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTgt                                                 <  1:4894170/71‑1 (MQ=255)
                     cGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTgtc                                                <  1:3089825/71‑1 (MQ=255)
                     cGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTgtc                                                <  1:3884070/71‑1 (MQ=255)
                     cGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTgtc                                                <  1:27048/71‑1 (MQ=255)
                         gCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTgtcaggt                                            <  1:1428624/71‑1 (MQ=255)
                           aTTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCgg                                                             <  1:5109511/52‑2 (MQ=21)
                           aTTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCat                                                           >  1:3028239/1‑54 (MQ=255)
                           aTTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTgtcaggtca                                          <  1:2544896/71‑1 (MQ=255)
                            ttcttcAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGt                                                                      <  1:255846/42‑1 (MQ=255)
                            ttcttcAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCa                                                            <  1:3372986/52‑1 (MQ=255)
                            ttcttcAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTgtcaggtcag                                         <  1:905760/71‑1 (MQ=255)
                              cttcAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCg                                                              <  1:1806517/48‑1 (MQ=255)
                              cttcAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCg                                                              <  1:3533528/48‑1 (MQ=255)
                              cttcAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCg                                                              <  1:1146509/48‑1 (MQ=255)
                              cttcAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCata                                                          >  1:1012614/1‑52 (MQ=255)
                              cttcAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGa                                       >  1:378251/1‑71 (MQ=255)
                                 cAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATgg                                    >  1:2876018/1‑71 (MQ=255)
                                  aGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATgg                                    <  1:5588450/70‑1 (MQ=255)
                                  aGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATgg                                    <  1:3533815/70‑1 (MQ=255)
                                  aGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATgg                                    <  1:3754195/70‑1 (MQ=255)
                                  aGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATgg                                    >  1:2151260/1‑70 (MQ=255)
                                  aGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATgg                                    <  1:144495/70‑1 (MQ=255)
                                   gATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGc                                                             <  1:5465470/44‑1 (MQ=17)
                                   gATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGc                                                             <  1:4431313/44‑1 (MQ=17)
                                   gATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGc                                                             <  1:2800937/44‑1 (MQ=17)
                                   gATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTgtc                                                <  1:2368326/57‑1 (MQ=255)
                                   gATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTg                                                  <  1:3524858/55‑1 (MQ=255)
                                   gATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTa                                  <  1:267666/71‑1 (MQ=255)
                                    aTGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGc                                                             <  1:1343803/43‑1 (MQ=17)
                                    aTGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTaa                                 <  1:5298051/71‑1 (MQ=255)
                                     tGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGccg                                                           >  1:2353590/1‑42 (MQ=17)
                                       gTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGc                              <  1:3600188/71‑1 (MQ=255)
                                       gTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGc                              <  1:146877/71‑1 (MQ=255)
                                         tCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCAc                            >  1:1017424/1‑71 (MQ=255)
                                          cAACCCTTAAGTTAGCGCTTATGGGAGTAATGCCCGCATATCCGGTTGTCAGGTCAGGATGTTa                                  <  1:2695067/64‑1 (MQ=255)
                                          cAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCAcc                           >  1:5314354/1‑71 (MQ=255)
                                           aaCCCTTAAGTTAGCGCTTATGGGAGTAATCCCAGCATATCCgg                                                     >  1:1412841/1‑44 (MQ=255)
                                            aCCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCgg                                                     >  1:4019836/1‑43 (MQ=255)
                                             cccTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCa                             <  1:3420571/66‑1 (MQ=255)
                                             cccTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGc                        >  1:3995604/1‑71 (MQ=255)
                                              ccTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGt                                                    <  1:4715684/42‑1 (MQ=255)
                                              ccTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCt                       >  1:4766369/1‑71 (MQ=255)
                                               cTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCtt                      <  1:2503859/71‑1 (MQ=255)
                                               cTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCtt                      >  1:4162522/1‑71 (MQ=255)
                                                ttAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTgtca                                               >  1:3701311/1‑45 (MQ=255)
                                                ttAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCtt                      >  1:4848505/1‑70 (MQ=255)
                                                 tAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTgtcaggtcag                                         <  1:1210304/50‑1 (MQ=255)
                                                 tAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTgtcaggtcag                                         <  1:4725854/50‑1 (MQ=255)
                                                 tAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTa                    >  1:465375/1‑71 (MQ=255)
                                                  aaGTTAGCGCTTATGGGAGTAATCCCCTCATATCCGGTTGTCAGGTCAGGATGGTAAGGc                              <  1:5327972/60‑1 (MQ=255)
                                                  aaGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGc                        <  1:4638110/66‑1 (MQ=255)
                                                  aaGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCt                       >  1:1625373/1‑67 (MQ=255)
                                                  aaGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTa                    <  1:5478611/70‑1 (MQ=255)
                                                   aGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTaca                  <  1:1440890/71‑1 (MQ=255)
                                                   aGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTac                   <  1:2728396/70‑1 (MQ=255)
                                                    gTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGa                                       <  1:2345616/49‑1 (MQ=255)
                                                    gTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTacac                 <  1:3171166/71‑1 (MQ=255)
                                                    gTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTacac                 <  1:1399369/71‑1 (MQ=255)
                                                      tAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACtt               >  1:4479509/1‑71 (MQ=255)
                                                       aGCGCTTATGGTAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACt                <  1:3006761/69‑1 (MQ=255)
                                                       aGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTAGACttt              >  1:2661505/1‑71 (MQ=255)
                                                       aGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACttt              >  1:4535145/1‑71 (MQ=255)
                                                           cTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGcc          <  1:332597/71‑1 (MQ=255)
                                                              aTGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGcc          >  1:2834742/1‑68 (MQ=255)
                                                              aTGGGAGTAATCCCCGCATATCCGGTTGTCAGGGCAGGATGGTAAGGCACCTGCTTTACACTTTc             <  1:504395/65‑1 (MQ=255)
                                                                 ggAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTgg     <  1:1982361/70‑1 (MQ=255)
                                                                 ggAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGt    <  1:2473681/71‑1 (MQ=255)
                                                                   aGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCa  <  1:2705510/71‑1 (MQ=255)
                                                                   aGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCa  <  1:3105773/71‑1 (MQ=255)
                                                                    gTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTc             >  1:5163661/1‑59 (MQ=255)
                                                                    gTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTc             >  1:1868960/1‑59 (MQ=255)
                                                                    gTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGt       >  1:1447809/1‑65 (MQ=255)
                                                                    gTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGAACCTGCTTTACACTTTc             >  1:2947952/1‑59 (MQ=255)
                                                                    |                                                                     
CGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGAGTAATCCCCGCATATCCGGTTGTCAGGTCAGGATGGTAAGGCACCTGCTTTACACTTTCGCCCGTGGTCA  >  Exported/364181‑364318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: