Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,057 C→T 9.7% F89F (TTC→TTT cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,0570CT 9.7% 225.5 / 11.4 82F89F (TTC→TTTcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base C (25/49);  new base T (3/5);  total (28/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

GCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGC  >  Exported/384988‑385126
                                                                     |                                                                     
gcgTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCg                                                                      <  1:4248013/71‑1 (MQ=255)
gcgTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCg                                                                      <  1:3563430/71‑1 (MQ=255)
 cgTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCg                                                                      <  1:1807338/70‑1 (MQ=255)
  gTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGt                                                                    <  1:2043370/71‑1 (MQ=255)
     tGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCAc                                                                 >  1:575409/1‑71 (MQ=255)
     tGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCAc                                                                 >  1:4054576/1‑71 (MQ=255)
         gCCAGCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCAtt                                                             <  1:506824/71‑1 (MQ=255)
             gCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGc                                                         <  1:1271483/71‑1 (MQ=255)
                ctGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGc                                                      <  1:565044/70‑1 (MQ=255)
                   cgcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCAcc                                                                >  1:4132910/1‑58 (MQ=255)
                   cgcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCa                                                    <  1:2759067/70‑1 (MQ=255)
                   cgcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAt                                                   >  1:4927011/1‑71 (MQ=255)
                    gcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt                                                  <  1:2971558/71‑1 (MQ=255)
                     ccATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGt                                                                    >  1:5505467/1‑52 (MQ=255)
                     ccATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGc                                                      <  1:3791880/66‑1 (MQ=255)
                     ccATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGc                                                      <  1:4333570/66‑1 (MQ=255)
                      cATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc                                                <  1:525246/71‑1 (MQ=255)
                      cATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc                                                <  1:3267131/71‑1 (MQ=255)
                      cATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc                                                <  1:1894336/71‑1 (MQ=255)
                      cATAACGACGGGCTGGACTATGTGCCGAAGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc                                                <  1:4955664/71‑1 (MQ=255)
                       aTAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGcc                                               <  1:1730019/71‑1 (MQ=255)
                        tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt                                                  <  1:967651/67‑1 (MQ=255)
                        tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt                                                  <  1:5657472/67‑1 (MQ=255)
                        tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt                                                  <  1:5153350/67‑1 (MQ=255)
                        tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt                                                  <  1:5663818/67‑1 (MQ=255)
                        tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCg                                              <  1:2051506/71‑1 (MQ=255)
                          acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCg                                                                      >  1:5500027/1‑45 (MQ=255)
                          acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCAtt                                                             >  1:2071143/1‑54 (MQ=255)
                          acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCAtt                                                             >  1:1304091/1‑54 (MQ=255)
                          acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCa                                                    >  1:4797923/1‑63 (MQ=255)
                          acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCggc                                            >  1:1702531/1‑70 (MQ=255)
                          acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCgg                                             <  1:781360/70‑1 (MQ=255)
                          acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCgg                                             <  1:1060206/70‑1 (MQ=255)
                          acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATATTGCGGCCATTGCCg                                              >  1:370272/1‑69 (MQ=255)
                           cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                           >  1:3004488/1‑71 (MQ=255)
                           cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                           <  1:1969869/71‑1 (MQ=255)
                           cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                           <  1:2937430/71‑1 (MQ=255)
                           cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                           <  1:2894153/71‑1 (MQ=255)
                           cgacgGACTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                           >  1:739857/1‑71 (MQ=255)
                            gacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCAtttt                                                           >  1:5138426/1‑54 (MQ=255)
                            gacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGc                                          <  1:2977286/71‑1 (MQ=255)
                             acgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCa                                                    >  1:2245817/1‑60 (MQ=255)
                             acgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                           <  1:1323330/69‑1 (MQ=255)
                              cgGGCTGGACTATGTGCCGACGGACAGGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg                                        <  1:744356/71‑1 (MQ=255)
                              cgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg                                        <  1:5272378/71‑1 (MQ=255)
                              cgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg                                        <  1:5164516/71‑1 (MQ=255)
                               gggCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAgg                                       <  1:19354/71‑1 (MQ=255)
                               gggCTGGACTATGTGCAGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAgg                                       <  1:2840582/71‑1 (MQ=255)
                                  cTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTcc                                    <  1:3776807/71‑1 (MQ=255)
                                   tGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCAt                                                              >  1:1040396/1‑44 (MQ=255)
                                      aCTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGa                                            <  1:3179177/59‑1 (MQ=255)
                                      aCTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtg                                <  1:3968233/71‑1 (MQ=255)
                                        tATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggt                              >  1:1513531/1‑71 (MQ=255)
                                          tgtgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTg                                                          >  1:3319642/1‑41 (MQ=255)
                                          tgtgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggtg                             <  1:2850864/70‑1 (MQ=255)
                                            tgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGcc                                                     <  1:1883475/44‑1 (MQ=255)
                                            tgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGcc                                                     <  1:2117544/44‑1 (MQ=255)
                                            tgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGcc                                                     <  1:3224693/44‑1 (MQ=255)
                                              ccGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGcc                        >  1:489017/1‑71 (MQ=255)
                                               cGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCg                       >  1:4572003/1‑71 (MQ=255)
                                                gACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCgg                      <  1:1661392/71‑1 (MQ=255)
                                                gACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCgg                      <  1:554019/71‑1 (MQ=255)
                                                 aCGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCa                                                    >  1:5182327/1‑40 (MQ=255)
                                                  ctgACAAGAAAGTGCTGTTCGGGCACCATTTTGCGGCCATTGCCGGAGCAGGGCCGCTGGTGGGGcc                        <  1:3712220/65‑1 (MQ=255)
                                                   ggACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtg                                >  1:2584290/1‑58 (MQ=255)
                                                   ggACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTAc                   >  1:1046367/1‑71 (MQ=255)
                                                      cAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTgg                <  1:2712877/71‑1 (MQ=255)
                                                       aaGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTggc               >  1:3787673/1‑71 (MQ=255)
                                                             gtgtTGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                              <  1:2651637/50‑1 (MQ=255)
                                                             gtgtTGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                              <  1:1850922/50‑1 (MQ=255)
                                                             gTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCa         <  1:1296041/71‑1 (MQ=255)
                                                             gTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCa         <  1:1289279/71‑1 (MQ=255)
                                                              tGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCg                                   <  1:33002/44‑1 (MQ=255)
                                                              tGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCg                                   <  1:4700197/44‑1 (MQ=255)
                                                               gttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCgccgcg           <  1:1649463/65‑1 (MQ=255)
                                                                ttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                              >  1:2677279/2‑47 (MQ=255)
                                                                ttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                              >  1:4289989/2‑47 (MQ=255)
                                                                cTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAAt      >  1:868934/1‑71 (MQ=255)
                                                                 tGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCa         >  1:3884430/1‑67 (MQ=255)
                                                                 tGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAAt      <  1:5489501/70‑1 (MQ=255)
                                                                  gTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATg     <  1:5612341/70‑1 (MQ=255)
                                                                  gTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATg     <  1:5279289/70‑1 (MQ=255)
                                                                     cGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGc  >  1:2273881/1‑70 (MQ=255)
                                                                     |                                                                     
GCGTATGACGCCAGCGGTGCGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGC  >  Exported/384988‑385126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: