Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,075 C→T 17.1% A95A (GCC→GCT cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,0750CT17.1% 169.2 / 24.3 76A95A (GCC→GCTcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base C (22/41);  new base T (5/8);  total (27/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

CGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATC  >  Exported/385007‑385144
                                                                    |                                                                     
cgcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCa                                                                      <  1:2759067/70‑1 (MQ=255)
cgcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAt                                                                     >  1:4927011/1‑71 (MQ=255)
 gcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt                                                                    <  1:2971558/71‑1 (MQ=255)
   cATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc                                                                  <  1:525246/71‑1 (MQ=255)
   cATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc                                                                  <  1:3267131/71‑1 (MQ=255)
   cATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc                                                                  <  1:1894336/71‑1 (MQ=255)
   cATAACGACGGGCTGGACTATGTGCCGAAGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc                                                                  <  1:4955664/71‑1 (MQ=255)
    aTAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGcc                                                                 <  1:1730019/71‑1 (MQ=255)
     tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt                                                                    <  1:967651/67‑1 (MQ=255)
     tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt                                                                    <  1:5663818/67‑1 (MQ=255)
     tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt                                                                    <  1:5657472/67‑1 (MQ=255)
     tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt                                                                    <  1:5153350/67‑1 (MQ=255)
     tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCg                                                                <  1:2051506/71‑1 (MQ=255)
       acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCa                                                                      >  1:4797923/1‑63 (MQ=255)
       acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCggc                                                              >  1:1702531/1‑70 (MQ=255)
       acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCgg                                                               <  1:781360/70‑1 (MQ=255)
       acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCgg                                                               <  1:1060206/70‑1 (MQ=255)
       acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATATTGCGGCCATTGCCg                                                                >  1:370272/1‑69 (MQ=255)
        cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                             <  1:1969869/71‑1 (MQ=255)
        cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                             >  1:3004488/1‑71 (MQ=255)
        cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                             <  1:2937430/71‑1 (MQ=255)
        cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                             <  1:2894153/71‑1 (MQ=255)
        cgacgGACTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                             >  1:739857/1‑71 (MQ=255)
         gacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGc                                                            <  1:2977286/71‑1 (MQ=255)
          acgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCa                                                                      >  1:2245817/1‑60 (MQ=255)
          acgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                             <  1:1323330/69‑1 (MQ=255)
           cgGGCTGGACTATGTGCCGACGGACAGGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg                                                          <  1:744356/71‑1 (MQ=255)
           cgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg                                                          <  1:5272378/71‑1 (MQ=255)
           cgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg                                                          <  1:5164516/71‑1 (MQ=255)
            gggCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAgg                                                         <  1:19354/71‑1 (MQ=255)
            gggCTGGACTATGTGCAGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAgg                                                         <  1:2840582/71‑1 (MQ=255)
               cTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTcc                                                      <  1:3776807/71‑1 (MQ=255)
                   aCTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGa                                                              <  1:3179177/59‑1 (MQ=255)
                   aCTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtg                                                  <  1:3968233/71‑1 (MQ=255)
                     tATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggt                                                >  1:1513531/1‑71 (MQ=255)
                       tgtgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggtg                                               <  1:2850864/70‑1 (MQ=255)
                         tgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGcc                                                                       <  1:3224693/44‑1 (MQ=255)
                         tgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGcc                                                                       <  1:2117544/44‑1 (MQ=255)
                         tgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGcc                                                                       <  1:1883475/44‑1 (MQ=255)
                           ccGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGcc                                          >  1:489017/1‑71 (MQ=255)
                            cGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCg                                         >  1:4572003/1‑71 (MQ=255)
                             gACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCgg                                        <  1:1661392/71‑1 (MQ=255)
                             gACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCgg                                        <  1:554019/71‑1 (MQ=255)
                              aCGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCa                                                                      >  1:5182327/1‑40 (MQ=255)
                               ctgACAAGAAAGTGCTGTTCGGGCACCATTTTGCGGCCATTGCCGGAGCAGGGCCGCTGGTGGGGcc                                          <  1:3712220/65‑1 (MQ=255)
                                ggACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtg                                                  >  1:2584290/1‑58 (MQ=255)
                                ggACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTAc                                     >  1:1046367/1‑71 (MQ=255)
                                   cAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTgg                                  <  1:2712877/71‑1 (MQ=255)
                                    aaGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTggc                                 >  1:3787673/1‑71 (MQ=255)
                                          gtgtTGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                                                <  1:1850922/50‑1 (MQ=255)
                                          gtgtTGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                                                <  1:2651637/50‑1 (MQ=255)
                                          gTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCa                           <  1:1296041/71‑1 (MQ=255)
                                          gTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCa                           <  1:1289279/71‑1 (MQ=255)
                                           tGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCg                                                     <  1:33002/44‑1 (MQ=255)
                                           tGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCg                                                     <  1:4700197/44‑1 (MQ=255)
                                            gttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCgccgcg                             <  1:1649463/65‑1 (MQ=255)
                                             ttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                                                >  1:2677279/2‑47 (MQ=255)
                                             ttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                                                >  1:4289989/2‑47 (MQ=255)
                                             cTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAAt                        >  1:868934/1‑71 (MQ=255)
                                              tGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCa                           >  1:3884430/1‑67 (MQ=255)
                                              tGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAAt                        <  1:5489501/70‑1 (MQ=255)
                                               gTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATg                       <  1:5612341/70‑1 (MQ=255)
                                               gTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATg                       <  1:5279289/70‑1 (MQ=255)
                                                 ttggTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGc                    >  1:230749/3‑71 (MQ=255)
                                                  cGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGc                    >  1:2273881/1‑70 (MQ=255)
                                                   ggTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCt                   <  1:1631204/70‑1 (MQ=255)
                                                    gTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGc                    >  1:204562/1‑68 (MQ=255)
                                                      cACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGt                                       <  1:1850941/47‑1 (MQ=255)
                                                      cACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGt                                       <  1:625748/47‑1 (MQ=255)
                                                       aCCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTg              >  1:4949266/1‑71 (MQ=255)
                                                       aCCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCt               <  1:3629173/70‑1 (MQ=255)
                                                        ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCt                   <  1:273080/65‑1 (MQ=255)
                                                        ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCt                   <  1:4808523/65‑1 (MQ=255)
                                                        ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGc             >  1:3509648/1‑71 (MQ=255)
                                                           ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCt                   <  1:647690/62‑1 (MQ=255)
                                                           ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg          >  1:5385301/1‑71 (MQ=255)
                                                           ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg          >  1:373704/1‑71 (MQ=255)
                                                             ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCggg         >  1:2082745/1‑70 (MQ=255)
                                                             ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCggg         >  1:283420/1‑70 (MQ=255)
                                                                   ccATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATc  >  1:724685/1‑71 (MQ=255)
                                                                    cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACt                                    <  1:4810106/36‑1 (MQ=255)
                                                                    |                                                                     
CGCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATC  >  Exported/385007‑385144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: