Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,084 A→T 22.4% G98G (GGA→GGT cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,0840AT22.4% 126.2 / 30.1 62G98G (GGA→GGTcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base A (18/30);  new base T (5/9);  total (23/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGC  >  Exported/385015‑385151
                                                                     |                                                                   
cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                                    <  1:2937430/71‑1 (MQ=255)
cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                                    >  1:3004488/1‑71 (MQ=255)
cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                                    <  1:2894153/71‑1 (MQ=255)
cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                                    <  1:1969869/71‑1 (MQ=255)
cgacgGACTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                                    >  1:739857/1‑71 (MQ=255)
 gacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGc                                                                   <  1:2977286/71‑1 (MQ=255)
  acgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                                    <  1:1323330/69‑1 (MQ=255)
   cgGGCTGGACTATGTGCCGACGGACAGGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg                                                                 <  1:744356/71‑1 (MQ=255)
   cgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg                                                                 <  1:5272378/71‑1 (MQ=255)
   cgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg                                                                 <  1:5164516/71‑1 (MQ=255)
    gggCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAgg                                                                <  1:19354/71‑1 (MQ=255)
    gggCTGGACTATGTGCAGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAgg                                                                <  1:2840582/71‑1 (MQ=255)
       cTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTcc                                                             <  1:3776807/71‑1 (MQ=255)
           aCTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGa                                                                     <  1:3179177/59‑1 (MQ=255)
           aCTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtg                                                         <  1:3968233/71‑1 (MQ=255)
             tATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggt                                                       >  1:1513531/1‑71 (MQ=255)
               tgtgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggtg                                                      <  1:2850864/70‑1 (MQ=255)
                   ccGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGcc                                                 >  1:489017/1‑71 (MQ=255)
                    cGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCg                                                >  1:4572003/1‑71 (MQ=255)
                     gACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCgg                                               <  1:1661392/71‑1 (MQ=255)
                     gACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCgg                                               <  1:554019/71‑1 (MQ=255)
                       ctgACAAGAAAGTGCTGTTCGGGCACCATTTTGCGGCCATTGCCGGAGCAGGGCCGCTGGTGGGGcc                                                 <  1:3712220/65‑1 (MQ=255)
                        ggACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtg                                                         >  1:2584290/1‑58 (MQ=255)
                        ggACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTAc                                            >  1:1046367/1‑71 (MQ=255)
                           cAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTgg                                         <  1:2712877/71‑1 (MQ=255)
                            aaGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTggc                                        >  1:3787673/1‑71 (MQ=255)
                                  gtgtTGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                                                       <  1:2651637/50‑1 (MQ=255)
                                  gtgtTGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                                                       <  1:1850922/50‑1 (MQ=255)
                                  gTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCa                                  <  1:1289279/71‑1 (MQ=255)
                                  gTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCa                                  <  1:1296041/71‑1 (MQ=255)
                                   tGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCg                                                            <  1:33002/44‑1 (MQ=255)
                                   tGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCg                                                            <  1:4700197/44‑1 (MQ=255)
                                    gttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCgccgcg                                    <  1:1649463/65‑1 (MQ=255)
                                     ttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                                                       >  1:4289989/2‑47 (MQ=255)
                                     ttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                                                       >  1:2677279/2‑47 (MQ=255)
                                     cTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAAt                               >  1:868934/1‑71 (MQ=255)
                                      tGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCa                                  >  1:3884430/1‑67 (MQ=255)
                                      tGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAAt                               <  1:5489501/70‑1 (MQ=255)
                                       gTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATg                              <  1:5279289/70‑1 (MQ=255)
                                       gTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATg                              <  1:5612341/70‑1 (MQ=255)
                                         ttggTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGc                           >  1:230749/3‑71 (MQ=255)
                                          cGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGc                           >  1:2273881/1‑70 (MQ=255)
                                           ggTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCt                          <  1:1631204/70‑1 (MQ=255)
                                            gTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGc                           >  1:204562/1‑68 (MQ=255)
                                              cACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGt                                              <  1:625748/47‑1 (MQ=255)
                                              cACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGt                                              <  1:1850941/47‑1 (MQ=255)
                                               aCCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTg                     >  1:4949266/1‑71 (MQ=255)
                                               aCCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCt                      <  1:3629173/70‑1 (MQ=255)
                                                ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCt                          <  1:4808523/65‑1 (MQ=255)
                                                ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCt                          <  1:273080/65‑1 (MQ=255)
                                                ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGc                    >  1:3509648/1‑71 (MQ=255)
                                                   ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCt                          <  1:647690/62‑1 (MQ=255)
                                                   ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg                 >  1:5385301/1‑71 (MQ=255)
                                                   ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg                 >  1:373704/1‑71 (MQ=255)
                                                     ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCggg                >  1:283420/1‑70 (MQ=255)
                                                     ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCggg                >  1:2082745/1‑70 (MQ=255)
                                                           ccATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATc         >  1:724685/1‑71 (MQ=255)
                                                            cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACt                                           <  1:4810106/36‑1 (MQ=255)
                                                             aTTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACt                                           <  1:3462893/35‑1 (MQ=255)
                                                             aTTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACt                                           <  1:1446467/35‑1 (MQ=255)
                                                                gCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTa                         >  1:2431413/1‑50 (MQ=255)
                                                                 ccGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgctg   <  1:3752196/71‑1 (MQ=255)
                                                                  cGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGTGCAGATGGGCTACCTGCCTGGCACGCTGTGgctgc  <  1:2802760/71‑1 (MQ=255)
                                                                     |                                                                   
CGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGC  >  Exported/385015‑385151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: