Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,087 A→T 24.1% A99A (GCA→GCT cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,0870AT24.1% 110.8 / 32.6 58A99A (GCA→GCTcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base A (18/26);  new base T (5/9);  total (23/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCT  >  Exported/385018‑385156
                                                                     |                                                                     
cgGGCTGGACTATGTGCCGACGGACAGGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg                                                                      <  1:744356/71‑1 (MQ=255)
cgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg                                                                      <  1:5272378/71‑1 (MQ=255)
cgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg                                                                      <  1:5164516/71‑1 (MQ=255)
 gggCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAgg                                                                     <  1:19354/71‑1 (MQ=255)
 gggCTGGACTATGTGCAGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAgg                                                                     <  1:2840582/71‑1 (MQ=255)
    cTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTcc                                                                  <  1:3776807/71‑1 (MQ=255)
        aCTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtg                                                              <  1:3968233/71‑1 (MQ=255)
          tATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggt                                                            >  1:1513531/1‑71 (MQ=255)
            tgtgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggtg                                                           <  1:2850864/70‑1 (MQ=255)
                ccGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGcc                                                      >  1:489017/1‑71 (MQ=255)
                 cGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCg                                                     >  1:4572003/1‑71 (MQ=255)
                  gACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCgg                                                    <  1:554019/71‑1 (MQ=255)
                  gACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCgg                                                    <  1:1661392/71‑1 (MQ=255)
                    ctgACAAGAAAGTGCTGTTCGGGCACCATTTTGCGGCCATTGCCGGAGCAGGGCCGCTGGTGGGGcc                                                      <  1:3712220/65‑1 (MQ=255)
                     ggACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtg                                                              >  1:2584290/1‑58 (MQ=255)
                     ggACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTAc                                                 >  1:1046367/1‑71 (MQ=255)
                        cAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTgg                                              <  1:2712877/71‑1 (MQ=255)
                         aaGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTggc                                             >  1:3787673/1‑71 (MQ=255)
                               gtgtTGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                                                            <  1:1850922/50‑1 (MQ=255)
                               gtgtTGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                                                            <  1:2651637/50‑1 (MQ=255)
                               gTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCa                                       <  1:1296041/71‑1 (MQ=255)
                               gTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCa                                       <  1:1289279/71‑1 (MQ=255)
                                tGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCg                                                                 <  1:33002/44‑1 (MQ=255)
                                tGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCg                                                                 <  1:4700197/44‑1 (MQ=255)
                                 gttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCgccgcg                                         <  1:1649463/65‑1 (MQ=255)
                                  ttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                                                            >  1:2677279/2‑47 (MQ=255)
                                  ttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                                                            >  1:4289989/2‑47 (MQ=255)
                                  cTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAAt                                    >  1:868934/1‑71 (MQ=255)
                                   tGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCa                                       >  1:3884430/1‑67 (MQ=255)
                                   tGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAAt                                    <  1:5489501/70‑1 (MQ=255)
                                    gTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATg                                   <  1:5279289/70‑1 (MQ=255)
                                    gTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATg                                   <  1:5612341/70‑1 (MQ=255)
                                      ttggTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGc                                >  1:230749/3‑71 (MQ=255)
                                       cGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGc                                >  1:2273881/1‑70 (MQ=255)
                                        ggTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCt                               <  1:1631204/70‑1 (MQ=255)
                                         gTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGc                                >  1:204562/1‑68 (MQ=255)
                                           cACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGt                                                   <  1:625748/47‑1 (MQ=255)
                                           cACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGt                                                   <  1:1850941/47‑1 (MQ=255)
                                            aCCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTg                          >  1:4949266/1‑71 (MQ=255)
                                            aCCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCt                           <  1:3629173/70‑1 (MQ=255)
                                             ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCt                               <  1:273080/65‑1 (MQ=255)
                                             ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCt                               <  1:4808523/65‑1 (MQ=255)
                                             ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGc                         >  1:3509648/1‑71 (MQ=255)
                                                ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCt                               <  1:647690/62‑1 (MQ=255)
                                                ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg                      >  1:373704/1‑71 (MQ=255)
                                                ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg                      >  1:5385301/1‑71 (MQ=255)
                                                  ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCggg                     >  1:283420/1‑70 (MQ=255)
                                                  ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCggg                     >  1:2082745/1‑70 (MQ=255)
                                                        ccATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATc              >  1:724685/1‑71 (MQ=255)
                                                         cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACt                                                <  1:4810106/36‑1 (MQ=255)
                                                          aTTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACt                                                <  1:1446467/35‑1 (MQ=255)
                                                          aTTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACt                                                <  1:3462893/35‑1 (MQ=255)
                                                             gCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTa                              >  1:2431413/1‑50 (MQ=255)
                                                              ccGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgctg        <  1:3752196/71‑1 (MQ=255)
                                                               cGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGTGCAGATGGGCTACCTGCCTGGCACGCTGTGgctgc       <  1:2802760/71‑1 (MQ=255)
                                                                   gCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGgatgat               >  1:2519884/1‑59 (MQ=255)
                                                                   gCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGgatgat               >  1:3264642/1‑59 (MQ=255)
                                                                    cAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCt  <  1:1548247/71‑1 (MQ=255)
                                                                     |                                                                     
CGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCT  >  Exported/385018‑385156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: