Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,114 G→C 34.8% A108A (GCG→GCC cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,1140GC34.8% 44.3 / 39.7 46A108A (GCG→GCCcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (15/15);  new base C (7/9);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 7.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.34e-01

GTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTG  >  Exported/385049‑385180
                                                                 |                                                                  
gTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCa                                                               <  1:1296041/71‑1 (MQ=255)
gTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCa                                                               <  1:1289279/71‑1 (MQ=255)
  gttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCgccgcg                                                                 <  1:1649463/65‑1 (MQ=255)
   cTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAAt                                                            >  1:868934/1‑71 (MQ=255)
    tGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCa                                                               >  1:3884430/1‑67 (MQ=255)
    tGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAAt                                                            <  1:5489501/70‑1 (MQ=255)
     gTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATg                                                           <  1:5279289/70‑1 (MQ=255)
     gTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATg                                                           <  1:5612341/70‑1 (MQ=255)
       ttggTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGc                                                        >  1:230749/3‑71 (MQ=255)
        cGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGc                                                        >  1:2273881/1‑70 (MQ=255)
         ggTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCt                                                       <  1:1631204/70‑1 (MQ=255)
          gTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGc                                                        >  1:204562/1‑68 (MQ=255)
             aCCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTg                                                  >  1:4949266/1‑71 (MQ=255)
             aCCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCt                                                   <  1:3629173/70‑1 (MQ=255)
              ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCt                                                       <  1:4808523/65‑1 (MQ=255)
              ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCt                                                       <  1:273080/65‑1 (MQ=255)
              ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGc                                                 >  1:3509648/1‑71 (MQ=255)
                 ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCt                                                       <  1:647690/62‑1 (MQ=255)
                 ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg                                              >  1:5385301/1‑71 (MQ=255)
                 ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg                                              >  1:373704/1‑71 (MQ=255)
                   ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCggg                                             >  1:283420/1‑70 (MQ=255)
                   ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCggg                                             >  1:2082745/1‑70 (MQ=255)
                         ccATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATc                                      >  1:724685/1‑71 (MQ=255)
                              gCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTa                                                      >  1:2431413/1‑50 (MQ=255)
                               ccGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgctg                                <  1:3752196/71‑1 (MQ=255)
                                cGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGTGCAGATGGGCTACCTGCCTGGCACGCTGTGgctgc                               <  1:2802760/71‑1 (MQ=255)
                                    gCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGgatgat                                       >  1:2519884/1‑59 (MQ=255)
                                    gCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGgatgat                                       >  1:3264642/1‑59 (MQ=255)
                                     cAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCt                          <  1:1548247/71‑1 (MQ=255)
                                       ggTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgggg                                            <  1:455446/51‑1 (MQ=255)
                                         tCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGcg                          >  1:4242236/1‑66 (MQ=255)
                                           cGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTAGCTGGggt                     <  1:4945212/70‑1 (MQ=255)
                                            gCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtgg                   <  1:2738382/71‑1 (MQ=255)
                                             cTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGggtcgt                  <  1:3437747/71‑1 (MQ=255)
                                                gtggGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGgct                                 <  1:499600/53‑1 (MQ=255)
                                                gtggGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTgct               >  1:449019/1‑71 (MQ=255)
                                                gtggGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTgct               >  1:3439234/1‑71 (MQ=255)
                                                gtggGTCCGGTTCGCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGgct                                 <  1:3781756/53‑1 (MQ=255)
                                                   ggtccGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGc            >  1:3274463/4‑71 (MQ=255)
                                                    ggCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGcc           <  1:5507184/71‑1 (MQ=255)
                                                         gTACTGGCGGCGCAAATGGGCTACCTGCCGGGgatg                                         <  1:1668014/36‑1 (MQ=255)
                                                         gTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCg                            <  1:1648172/49‑1 (MQ=255)
                                                          tACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtgg                   <  1:5656408/57‑1 (MQ=255)
                                                          tACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGCCTGCTCGCTGGggtgg                   <  1:3690331/57‑1 (MQ=255)
                                                           aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTgg                        >  1:4122070/1‑51 (MQ=255)
                                                           aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcg     >  1:4901219/1‑70 (MQ=255)
                                                            cTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTtctc              >  1:2271265/1‑60 (MQ=255)
                                                              ggcggcGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTtctc              >  1:3012489/1‑58 (MQ=255)
                                                               gcggcgCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcggtg  >  1:1625793/1‑69 (MQ=255)
                                                                 |                                                                  
GTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTG  >  Exported/385049‑385180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: