Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,186 T→C 14.8% D132D (GAT→GAC cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,1860TC14.8% 122.5 / 12.6 54D132D (GAT→GACcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base T (23/23);  new base C (2/6);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

GCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGG  >  Exported/385117‑385256
                                                                     |                                                                       
gcAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAtt                                                                        >  1:5187398/1‑71 (MQ=255)
 cagaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActt                                                                       <  1:4055455/68‑1 (MQ=255)
 cAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                                                                       <  1:1112480/71‑1 (MQ=255)
   gaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActtta                                                                     >  1:3683789/2‑69 (MQ=255)
   gaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActtta                                                                     <  1:5612495/70‑3 (MQ=255)
   gaTGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGActtta                                                                     <  1:1917284/70‑3 (MQ=255)
     tGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATg                                                                   <  1:4973445/71‑1 (MQ=255)
     tGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTAGCCGGTGCGGTGCAGGATTTCATg                                                                   >  1:5038970/1‑71 (MQ=255)
          tACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCt                                                              >  1:268300/1‑71 (MQ=255)
          tACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCt                                                              >  1:2429487/1‑71 (MQ=255)
            ccTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgt                                                            <  1:3102020/71‑1 (MQ=255)
             cTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtt                                                           >  1:4242997/1‑71 (MQ=255)
                ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                                                                       <  1:808785/56‑1 (MQ=255)
                ccGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgt                                                        >  1:4970713/1‑71 (MQ=255)
                  ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCa                                                                     >  1:890358/1‑56 (MQ=255)
                  ggggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCa                                                                     >  1:3114377/1‑56 (MQ=255)
                    ggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCt                                                    <  1:416204/71‑1 (MQ=255)
                    ggATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCt                                                    <  1:3221073/71‑1 (MQ=255)
                     gatgatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCt                                                    <  1:356398/70‑1 (MQ=255)
                        gatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTAcgc                                                >  1:4146158/1‑71 (MQ=255)
                         atCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                                                                       <  1:3721332/47‑1 (MQ=255)
                         atCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGAttt                                                                       <  1:3860205/47‑1 (MQ=255)
                            tGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGTTGCAGGAtt                                                                        <  1:3479728/43‑1 (MQ=255)
                              gctgctGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGt                                           <  1:1542498/70‑1 (MQ=255)
                              gctgctGGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGt                                           <  1:3090677/70‑1 (MQ=255)
                              gctgctGGCGGGGGGCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCTGTTTATCTCCTCTCGCCGt                                           <  1:500199/70‑1 (MQ=255)
                              gctgctCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGcc                                             <  1:4305117/68‑1 (MQ=255)
                              gctgctCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGt                                           <  1:3200192/70‑1 (MQ=255)
                              gctgctCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGt                                           <  1:4418362/70‑1 (MQ=255)
                              gctgctCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGt                                           <  1:2458152/70‑1 (MQ=255)
                               ctgctgGCGGGGGTCGTGCTGGCCGGTGCGGTTCAGGACTTTATGGTGCtgttt                                                          <  1:1922410/54‑1 (MQ=255)
                                    cGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtc                                    >  1:5490728/1‑71 (MQ=255)
                                     gCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcg                                   >  1:3046235/1‑71 (MQ=255)
                                        ggggTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGt                                     <  1:5310234/66‑1 (MQ=255)
                                         gggTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgctc                               <  1:1595512/70‑1 (MQ=255)
                                          ggtggtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGtcgctcgc                             >  1:3698662/1‑71 (MQ=255)
                                           gtggtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTcgctcgct                            <  1:1056214/71‑1 (MQ=255)
                                              gtTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTa                                                   <  1:1259021/46‑1 (MQ=255)
                                                tctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                        >  1:1874767/1‑70 (MQ=255)
                                                tctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                        >  1:4562062/1‑70 (MQ=255)
                                                tctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                        >  1:4414246/1‑70 (MQ=255)
                                                 ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTa                                                   >  1:5014995/1‑43 (MQ=255)
                                                 ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGt                        >  1:2785190/1‑69 (MQ=255)
                                                 ctcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTg                       <  1:614695/70‑1 (MQ=255)
                                                  tcGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTc                                                     <  1:365446/40‑1 (MQ=255)
                                                    gCCGGTGCGGTGCAGGATTTCATGGTGCtgtttgtt                                                       >  1:372742/1‑36 (MQ=255)
                                                    gCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt                                                      >  1:2460449/1‑37 (MQ=255)
                                                    gCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt                                                      >  1:2593068/1‑37 (MQ=255)
                                                    gCCGGTGCGGTGCAGGATTTCATGGTGCTgtttgttt                                                      >  1:400031/1‑37 (MQ=255)
                                                    gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTc                                                     <  1:5469044/38‑1 (MQ=255)
                                                    gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGc                    <  1:2879691/70‑1 (MQ=255)
                                                    gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCt                   >  1:2012251/1‑71 (MQ=255)
                                                     ccGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTg                  <  1:5132589/71‑1 (MQ=255)
                                                       ggtgcggtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGt                >  1:3763399/1‑71 (MQ=255)
                                                        gtgcggtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTc               <  1:1909713/71‑1 (MQ=255)
                                                         tgcggtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACgg                                      <  1:2536175/48‑1 (MQ=255)
                                                            ggtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAaag           <  1:1055205/71‑1 (MQ=255)
                                                             gtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGCTCGCTCGCTGGGTGAGCTGGTCAaaga          <  1:1892611/71‑1 (MQ=255)
                                                                 aGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTg                       <  1:5526870/54‑1 (MQ=255)
                                                                  ggATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTg                       <  1:3461564/53‑1 (MQ=255)
                                                                  ggACTTTATGGTGCTGTTTATCTCCTCTCGCCGTAATGGCGCATCTCTTGGTGAGATGATCAAAGAagag      <  1:1528555/70‑1 (MQ=255)
                                                                   gATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAg                     >  1:5634270/1‑54 (MQ=255)
                                                                   gATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAg                     >  1:2791026/1‑54 (MQ=255)
                                                                    aTTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATg   >  1:3716572/1‑71 (MQ=255)
                                                                     tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGt                                     >  1:3688679/1‑37 (MQ=255)
                                                                     tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGt                                     >  1:56949/1‑37 (MQ=255)
                                                                     tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGt                                     >  1:881978/1‑37 (MQ=255)
                                                                     tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg  >  1:3351490/1‑71 (MQ=255)
                                                                     tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATgg  <  1:3741813/71‑1 (MQ=255)
                                                                     |                                                                       
GCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGG  >  Exported/385117‑385256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: