Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,447 C→T 10.9% G219G (GGC→GGT cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,4470CT10.9% 169.6 / 9.3 64G219G (GGC→GGTcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base C (30/27);  new base T (3/4);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

TTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGAAAG  >  Exported/385377‑385515
                                                                      |                                                                    
ttGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGc                                                                      <  1:3186701/71‑1 (MQ=255)
   cGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGa                                                                    <  1:3754821/70‑1 (MQ=255)
       cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgt                                                               >  1:107078/1‑71 (MQ=255)
       cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgt                                                               >  1:4635427/1‑71 (MQ=255)
       cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtg                                                                >  1:5071817/1‑70 (MQ=255)
       cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtg                                                                <  1:1659745/70‑1 (MQ=255)
        aCCATTCCGCTGGCGCTGTTTATTGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc                                                              >  1:4454511/1‑71 (MQ=255)
            ttCCGCTGTCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgg                                                               <  1:163748/66‑2 (MQ=255)
            ttCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCg                                                                     <  1:2667754/60‑1 (MQ=255)
            ttCCGCTGGCGCTGTTTATGGGGATCTACCCGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCg                                                             <  1:3361179/68‑1 (MQ=255)
             tCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCa                                                         >  1:4031201/1‑71 (MQ=255)
                   tgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTc                                                          >  1:4899344/2‑64 (MQ=255)
                   tgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTc                                                          >  1:4975636/2‑64 (MQ=255)
                    gcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTGGta                                                   <  1:554944/70‑2 (MQ=255)
                    gcgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTc                                                   <  1:3730904/70‑1 (MQ=255)
                     cgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTGGtatc                                                 <  1:2715536/71‑4 (MQ=255)
                      gcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTgg                                                <  1:2204072/71‑1 (MQ=255)
                       cTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAgtct                                                               <  1:4865738/55‑3 (MQ=255)
                         gTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTgg                                                <  1:4519854/68‑1 (MQ=255)
                         gTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAt                                             >  1:814768/1‑71 (MQ=255)
                         gTTTATGGGGATCTACATGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAt                                             >  1:1186824/1‑71 (MQ=255)
                          tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAtt                                            <  1:914747/71‑1 (MQ=255)
                           ttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTa                                              >  1:671109/1‑68 (MQ=255)
                            tATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATc                                                       >  1:2601464/1‑58 (MQ=255)
                              tGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt                                                        >  1:2479440/1‑55 (MQ=255)
                              tGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTg                                        <  1:2598206/71‑1 (MQ=255)
                                gggATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAg                                                                  >  1:956833/1‑43 (MQ=255)
                                gggATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAg                                                                  >  1:5550889/1‑43 (MQ=255)
                                gggATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAg                                                                  >  1:4471752/1‑43 (MQ=255)
                                  gATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAttt                                    <  1:1930464/71‑1 (MQ=255)
                                   aTCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtg                                                                <  1:1589641/42‑1 (MQ=255)
                                   aTCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTg                                        <  1:1189991/66‑1 (MQ=255)
                                   aTCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTg                                        <  1:1950871/66‑1 (MQ=255)
                                    tCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAtttt                                   >  1:477107/1‑70 (MQ=255)
                                    tCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGaa                                                                   >  1:862661/1‑38 (MQ=255)
                                     cTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc                                                              >  1:353490/1‑42 (MQ=255)
                                     cTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc                                                              >  1:3118638/1‑42 (MQ=255)
                                      tACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGtctct                                                             <  1:2387922/42‑2 (MQ=255)
                                       aCCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTa                                              >  1:2881672/1‑56 (MQ=255)
                                         cTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCAt                             <  1:661134/71‑1 (MQ=255)
                                           gcgcTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTa                           >  1:1937530/1‑71 (MQ=255)
                                                 tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt                                                        >  1:321450/1‑36 (MQ=255)
                                                 tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAtt                                     <  1:3591105/55‑1 (MQ=255)
                                                 tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATctct                      <  1:32309/70‑1 (MQ=255)
                                                 tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATctct                      <  1:3178753/70‑1 (MQ=255)
                                                  cTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATctct                      >  1:1951846/1‑69 (MQ=255)
                                                  cTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTgg                    >  1:815464/1‑71 (MQ=255)
                                                     cGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTAtct                        >  1:943857/1‑64 (MQ=255)
                                                      gTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTggcggc                <  1:5626184/71‑1 (MQ=255)
                                                         cGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTgg             >  1:3061450/1‑71 (MQ=255)
                                                            ggCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTg              <  1:1438989/67‑1 (MQ=255)
                                                              cGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAtt                                     <  1:2619072/42‑1 (MQ=255)
                                                              cGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTggc                   >  1:2731161/1‑60 (MQ=255)
                                                                tATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCa       <  1:3305038/70‑1 (MQ=255)
                                                                  ttGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGa                                       >  1:1824701/1‑36 (MQ=255)
                                                                  ttGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGa                                       >  1:459473/1‑36 (MQ=255)
                                                                  ttGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGa                                       >  1:2394634/1‑36 (MQ=255)
                                                                  ttGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGaa    <  1:4021191/71‑1 (MQ=255)
                                                                  ttGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGa     <  1:5458560/70‑1 (MQ=255)
                                                                  ttGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGa     >  1:236611/1‑70 (MQ=255)
                                                                   tGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTggcggc                >  1:5110187/1‑58 (MQ=255)
                                                                   tGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTggg            <  1:4450582/62‑1 (MQ=255)
                                                                    ggCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGaaa   <  1:1217968/70‑1 (MQ=255)
                                                                    ggCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGAAAg  >  1:4846007/1‑71 (MQ=255)
                                                                     gCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGAAAg  <  1:4370921/70‑1 (MQ=255)
                                                                      |                                                                    
TTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGGCGGCTGGGTGGCAGAAAG  >  Exported/385377‑385515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: