Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,603 G→A 9.5% P271P (CCG→CCA cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,6030GA 9.5% 171.9 / 7.1 63P271P (CCG→CCAcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (28/29);  new base A (2/4);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

CTTTGACTTTACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTG  >  Exported/385534‑385672
                                                                     |                                                                     
cTTTGACTTTACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCgg                                                                      >  1:985229/1‑71 (MQ=255)
cTTTGACTTTACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCgg                                                                      >  1:2048730/1‑71 (MQ=255)
  ttGACTTTACCGGCGTGCAGGTGACCTGGATGCTGGTGGGATACGGTTTTGTGGCGGCGGTGCTGCCGgtg                                                                    >  1:3148826/1‑71 (MQ=255)
   tGACTTTACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCg                                                                       >  1:1637274/1‑67 (MQ=255)
    gACTTTACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGtgtg                                                                  >  1:1371010/1‑71 (MQ=255)
     aCTTTACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGtgtg                                                                  <  1:3895423/70‑1 (MQ=255)
     aCTTTACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGtgtg                                                                  <  1:4125503/70‑1 (MQ=255)
      cTTTACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGt                                                                <  1:4412872/71‑1 (MQ=255)
        ttACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTa                                                              >  1:4822375/1‑71 (MQ=255)
          aCCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTAct                                                            >  1:196598/1‑71 (MQ=255)
              gCGTGCAGTTGACCTGGATGCTGGTGGGTTACTGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTActgctg                                                        >  1:3156345/1‑71 (MQ=255)
              gCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTActgctg                                                        >  1:3949890/1‑71 (MQ=255)
                   cAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGccc                                                    <  1:1764602/70‑1 (MQ=255)
                    aGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCcg                                                  <  1:3206180/71‑1 (MQ=255)
                      ttGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGATGGCCCcccg                                                >  1:852744/1‑71 (MQ=255)
                        gACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGt                                               >  1:5671959/1‑70 (MQ=255)
                        gACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGt                                               >  1:1315308/1‑70 (MQ=255)
                          ccTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGAc                                            >  1:5090822/1‑71 (MQ=255)
                           cTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGt                                                                <  1:3908492/50‑1 (MQ=255)
                           cTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTAc                                                             <  1:311343/53‑1 (MQ=255)
                           cTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTAc                                                             <  1:851523/53‑1 (MQ=255)
                           cTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTAc                                                             <  1:1617529/53‑1 (MQ=255)
                            tGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGgtgt                                                                   <  1:701869/46‑1 (MQ=255)
                            tGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGgtgt                                                                   <  1:3586568/46‑1 (MQ=255)
                            tGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGgtgt                                                                   <  1:5578655/46‑1 (MQ=255)
                            tGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGt                                                                <  1:3426460/49‑1 (MQ=255)
                             ggATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTa                                                              >  1:573667/1‑50 (MQ=255)
                             ggATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTa                                                              >  1:759079/1‑50 (MQ=255)
                             ggATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAc                                         >  1:4665770/1‑71 (MQ=255)
                             ggATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCACCGCGTg                                              >  1:5331024/1‑66 (MQ=255)
                              gATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGcccc                                                   >  1:4819517/1‑60 (MQ=255)
                              gATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAcc                                        >  1:4752566/1‑71 (MQ=255)
                              gATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAcc                                        >  1:1069388/1‑71 (MQ=255)
                              gATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAc                                         <  1:4483431/70‑1 (MQ=255)
                               aTGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACct                                       >  1:4723507/1‑71 (MQ=255)
                                    ggtggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTa                                  >  1:144673/1‑71 (MQ=255)
                                    ggtggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTa                                  >  1:4706999/1‑71 (MQ=255)
                                     gtggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTAc                                 >  1:2173621/1‑71 (MQ=255)
                                      tggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGt                                                                <  1:2747739/39‑1 (MQ=255)
                                      tggGTTACGGTTTTGTGGCAGCGGTGCTGCCGGTGTGGTTActgctg                                                        <  1:2753046/47‑1 (MQ=255)
                                         gTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTAct                                                            <  1:1890492/40‑1 (MQ=255)
                                         gTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTc                             <  1:3594466/71‑1 (MQ=255)
                                           tACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCt                           >  1:556715/1‑71 (MQ=255)
                                           tACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCt                           >  1:4062551/1‑71 (MQ=255)
                                            aCGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAcc                                        >  1:4165621/1‑57 (MQ=255)
                                              ggTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGaa                        <  1:833257/71‑1 (MQ=255)
                                               gTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGaaa                       <  1:1130967/71‑1 (MQ=255)
                                                  ttGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTcc                            <  1:2931034/63‑1 (MQ=255)
                                                  ttGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                    >  1:3968050/1‑71 (MQ=255)
                                                          gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                    <  1:2092252/63‑1 (MQ=255)
                                                          gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                    <  1:3265248/63‑1 (MQ=255)
                                                          gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                    <  1:5542898/63‑1 (MQ=255)
                                                          gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                    <  1:5671157/63‑1 (MQ=255)
                                                           cactgctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGaaaa                      >  1:907676/4‑60 (MQ=255)
                                                              tgctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGa                         <  1:3358470/54‑1 (MQ=255)
                                                               gctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtcg       >  1:3116366/1‑71 (MQ=255)
                                                                ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTa             >  1:1879722/1‑64 (MQ=255)
                                                                ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg      >  1:3163480/1‑71 (MQ=255)
                                                                ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg      <  1:4568831/71‑1 (MQ=255)
                                                                ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg      >  1:1053986/1‑71 (MQ=255)
                                                                ctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtcg       <  1:5292467/70‑1 (MQ=255)
                                                                ctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtc        <  1:1211863/69‑1 (MQ=255)
                                                                ctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtc        <  1:5149104/69‑1 (MQ=255)
                                                                   ccGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGa                         <  1:1612282/49‑1 (MQ=255)
                                                                   ccGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg      <  1:5232363/68‑1 (MQ=255)
                                                                   ccGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg      <  1:1553514/68‑1 (MQ=255)
                                                                     ggTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTg  <  1:1446727/70‑1 (MQ=255)
                                                                     |                                                                     
CTTTGACTTTACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTG  >  Exported/385534‑385672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: