Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,627 T→C 9.5% R279R (CGT→CGC cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,6270TC 9.5% 165.9 / 7.3 63R279R (CGT→CGCcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base T (25/32);  new base C (2/4);  total (27/36)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

GACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGC  >  Exported/385558‑385696
                                                                     |                                                                     
gACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGt                                                                       >  1:5671959/1‑70 (MQ=255)
gACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGt                                                                       >  1:1315308/1‑70 (MQ=255)
  ccTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGAc                                                                    >  1:5090822/1‑71 (MQ=255)
     ggATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAc                                                                 >  1:4665770/1‑71 (MQ=255)
     ggATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCACCGCGTg                                                                      >  1:5331024/1‑66 (MQ=255)
      gATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAcc                                                                >  1:1069388/1‑71 (MQ=255)
      gATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAcc                                                                >  1:4752566/1‑71 (MQ=255)
      gATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAc                                                                 <  1:4483431/70‑1 (MQ=255)
       aTGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACct                                                               >  1:4723507/1‑71 (MQ=255)
            ggtggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTa                                                          >  1:144673/1‑71 (MQ=255)
            ggtggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTa                                                          >  1:4706999/1‑71 (MQ=255)
             gtggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTAc                                                         >  1:2173621/1‑71 (MQ=255)
                 gTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTc                                                     <  1:3594466/71‑1 (MQ=255)
                   tACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCt                                                   >  1:4062551/1‑71 (MQ=255)
                   tACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCt                                                   >  1:556715/1‑71 (MQ=255)
                    aCGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAcc                                                                >  1:4165621/1‑57 (MQ=255)
                      ggTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGaa                                                <  1:833257/71‑1 (MQ=255)
                       gTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGaaa                                               <  1:1130967/71‑1 (MQ=255)
                          ttGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTcc                                                    <  1:2931034/63‑1 (MQ=255)
                          ttGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                                            >  1:3968050/1‑71 (MQ=255)
                                  gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                                            <  1:5542898/63‑1 (MQ=255)
                                  gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                                            <  1:5671157/63‑1 (MQ=255)
                                  gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                                            <  1:3265248/63‑1 (MQ=255)
                                  gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                                            <  1:2092252/63‑1 (MQ=255)
                                   cactgctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGaaaa                                              >  1:907676/4‑60 (MQ=255)
                                      tgctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGa                                                 <  1:3358470/54‑1 (MQ=255)
                                       gctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtcg                               >  1:3116366/1‑71 (MQ=255)
                                        ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTa                                     >  1:1879722/1‑64 (MQ=255)
                                        ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg                              >  1:3163480/1‑71 (MQ=255)
                                        ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg                              <  1:4568831/71‑1 (MQ=255)
                                        ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg                              >  1:1053986/1‑71 (MQ=255)
                                        ctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtcg                               <  1:5292467/70‑1 (MQ=255)
                                        ctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtc                                <  1:5149104/69‑1 (MQ=255)
                                        ctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtc                                <  1:1211863/69‑1 (MQ=255)
                                           ccGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGa                                                 <  1:1612282/49‑1 (MQ=255)
                                           ccGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg                              <  1:1553514/68‑1 (MQ=255)
                                           ccGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg                              <  1:5232363/68‑1 (MQ=255)
                                             ggTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTg                          <  1:1446727/70‑1 (MQ=255)
                                               tgtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGc                        <  1:5483196/70‑1 (MQ=255)
                                               tgtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCg                       <  1:457091/71‑1 (MQ=255)
                                                gtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCgg                      >  1:2327305/1‑71 (MQ=255)
                                                 tgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGaa                                                <  1:2177273/44‑1 (MQ=255)
                                                   gTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATGGTCGGt                             <  1:5998/61‑1 (MQ=255)
                                                   gTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCTGTCTGGCGGTAg                   <  1:1606371/71‑1 (MQ=255)
                                                   gTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAg                   >  1:3797468/1‑71 (MQ=255)
                                                    ttACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAgg                  >  1:2483854/1‑71 (MQ=255)
                                                      aCTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                                            >  1:5690135/1‑43 (MQ=255)
                                                      aCTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGAc                                       >  1:2137469/1‑48 (MQ=255)
                                                      aCTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTg                          <  1:5039293/61‑1 (MQ=255)
                                                        tgctgGCCCCGCGTGACTACCTCTGTACCTTCCTGAAAATCGGGACTATCGTCGGt                             <  1:3512216/56‑1 (MQ=255)
                                                        tgctgGCCCCGCGTGACTACCTCTCTACCTTCCTGaaa                                               <  1:1940238/38‑1 (MQ=255)
                                                        tgctgGCCCCGCGTGACTACCTCTCTACCTTCCTGaaa                                               <  1:1899124/38‑1 (MQ=255)
                                                        tgctgGCCCCGCGTGACTACCTCTCTACCTTCCTGaaa                                               <  1:4448642/38‑1 (MQ=255)
                                                        tgctgGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGTGACTATCGTCGGt                             <  1:1516846/56‑1 (MQ=255)
                                                        tgctgGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGt                             <  1:2109879/56‑1 (MQ=255)
                                                          ctgGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCAtttt            >  1:2013277/1‑71 (MQ=255)
                                                          ctgGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCAtt              <  1:4871700/69‑1 (MQ=255)
                                                             gCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGAt         <  1:613552/71‑1 (MQ=255)
                                                             gCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGAt         <  1:3925491/71‑1 (MQ=255)
                                                                  gcgTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGAt         <  1:1150380/66‑1 (MQ=255)
                                                                  gcgTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGAt         <  1:4726105/66‑1 (MQ=255)
                                                                   cgTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCt                           >  1:4729189/1‑47 (MQ=255)
                                                                   cgTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTa       >  1:3422983/1‑67 (MQ=255)
                                                                    gTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATgcgc  >  1:2676299/1‑71 (MQ=255)
                                                                    gTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATgcgc  <  1:3111417/71‑1 (MQ=255)
                                                                     |                                                                     
GACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGC  >  Exported/385558‑385696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: