Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,771 G→C 6.0% P327P (CCG→CCC cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,7710GC 6.0% 306.9 / 6.8 99P327P (CCG→CCCcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (45/48);  new base C (4/2);  total (49/50)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 103 total aligned reads displayed.

GCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGC  >  Exported/385702‑385841
                                                                     |                                                                      
gCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGt                                                                       <  1:3114168/71‑1 (MQ=255)
gCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGt                                                                       >  1:2838784/1‑71 (MQ=255)
gCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGt                                                                       >  1:535308/1‑71 (MQ=255)
    aCCATGCCTGCGCTGACCATATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCt                                                                   <  1:1385834/71‑1 (MQ=255)
    aCCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCt                                                                   <  1:5466735/71‑1 (MQ=255)
     ccATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTg                                                                  <  1:2447508/71‑1 (MQ=255)
     ccATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTg                                                                  <  1:5018241/71‑1 (MQ=255)
      cATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGt                                                                       <  1:1014395/65‑1 (MQ=255)
      cATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGt                                                                       <  1:1150807/65‑1 (MQ=255)
        tGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGtt                                                                >  1:4092047/1‑70 (MQ=255)
        tGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGtt                                                                >  1:4400496/1‑70 (MQ=255)
        tGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGtt                                                                >  1:4470306/1‑70 (MQ=255)
         gCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGt                                                                       >  1:857077/1‑62 (MQ=255)
         gCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTa                                                              >  1:3015739/1‑71 (MQ=255)
          ccTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTAt                                                             >  1:267907/1‑71 (MQ=255)
          ccTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTAt                                                             >  1:1176196/1‑71 (MQ=255)
            tGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTAt                                                             <  1:5184631/69‑1 (MQ=255)
             gcgcTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCAc                                                          >  1:3218/1‑71 (MQ=255)
              cgcTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTg                                                                  >  1:2480994/1‑62 (MQ=255)
              cgcTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCAc                                                          >  1:5528319/1‑70 (MQ=255)
                   aCCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGc                                                    >  1:153575/1‑71 (MQ=255)
                    ccAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGcc                                                   <  1:412730/71‑1 (MQ=255)
                     cAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTAt                                                             >  1:1437279/1‑60 (MQ=255)
                       aaTTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtgt                                                >  1:704952/1‑71 (MQ=255)
                        aTTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtgtg                                               <  1:534802/71‑1 (MQ=255)
                          ttGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtga                                                >  1:5220164/1‑67 (MQ=255)
                          ttGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGgc                                             >  1:5664158/1‑71 (MQ=255)
                           tGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGgcg                                            >  1:454788/1‑71 (MQ=255)
                               gATTGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgt                                        <  1:5600569/71‑1 (MQ=255)
                               gATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGt                                                                       <  1:3974675/40‑1 (MQ=255)
                               gATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgt                                        <  1:2073417/71‑1 (MQ=255)
                                 tGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgtgt                                      <  1:381716/71‑1 (MQ=255)
                                 tGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgtg                                       >  1:757379/1‑70 (MQ=255)
                                  ggCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc                                     >  1:3297794/1‑71 (MQ=255)
                                  ggCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc                                     >  1:1851656/1‑71 (MQ=255)
                                   gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc                                     <  1:3043332/70‑1 (MQ=255)
                                   gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt                                    >  1:1195516/1‑71 (MQ=255)
                                   gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt                                    <  1:208726/71‑1 (MQ=255)
                                   gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt                                    <  1:3771173/71‑1 (MQ=255)
                                    cACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTg                                   >  1:1514140/1‑71 (MQ=255)
                                     aCTGGCCCGGTGTGGTCGGGCAATGTGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCgtc                                       <  1:5583916/66‑2 (MQ=255)
                                     aCTGGCCCGGTGTGGTCGGGCAATGTGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCgtc                                       <  1:3076186/66‑2 (MQ=255)
                                      cTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtg                                                 <  1:4638015/55‑1 (MQ=255)
                                      cTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtg                                                 <  1:3663082/55‑1 (MQ=255)
                                      cTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc                                 >  1:1656502/1‑71 (MQ=255)
                                      cTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc                                 >  1:3721522/1‑71 (MQ=255)
                                       tGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtg                                                 <  1:5119357/54‑1 (MQ=255)
                                        ggCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATc                                                            >  1:5134494/1‑42 (MQ=255)
                                         gCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCC‑‑TGGCGCGGTGTCTGGCTTc                              <  1:2628831/69‑1 (MQ=255)
                                             ggTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCt                                                  >  1:4413748/1‑47 (MQ=255)
                                               tATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCTtgtg                                               <  1:175695/48‑1 (MQ=255)
                                               tATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                              <  1:4039023/65‑1 (MQ=255)
                                                 tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgt                                        <  1:5547667/53‑1 (MQ=255)
                                                 tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc                                 <  1:5280871/60‑1 (MQ=255)
                                                   gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtg                                                 >  1:1997239/1‑42 (MQ=255)
                                                   gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg                     <  1:4515735/70‑1 (MQ=255)
                                                   gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg                     <  1:1045122/70‑1 (MQ=255)
                                                    aCCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCAGTGGCGCGGTGTCTgg                                  <  1:3608653/56‑1 (MQ=255)
                                                      cGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc                                     <  1:4457964/51‑1 (MQ=255)
                                                       ggTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAtctc                <  1:5356668/71‑1 (MQ=255)
                                                        gTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATctct               <  1:3691129/71‑1 (MQ=255)
                                                         tAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtctt              <  1:4003777/71‑1 (MQ=255)
                                                         tAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtctt              <  1:3370116/71‑1 (MQ=255)
                                                          aaCCTGTTCGCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc             >  1:3309006/1‑71 (MQ=255)
                                                          aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg                     >  1:1059454/1‑63 (MQ=255)
                                                          aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc             >  1:5359783/1‑71 (MQ=255)
                                                           aCCTGTTCCCGTTCCTGTTTATCACCATCGCCtgt                                                <  1:3837791/35‑1 (MQ=255)
                                                           aCCTGTTCCCGTTCCTGTTTATCACCATCGCCtgt                                                <  1:4042647/35‑1 (MQ=255)
                                                           aCCTGTTCCCGTTCCTGTTTATCACCATCGCCtgt                                                <  1:3098531/35‑1 (MQ=255)
                                                            tcTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGgt                                        <  1:652291/41‑1 (MQ=38)
                                                             cTGTTCCCGTTCCTGTTTTTCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                              <  1:754599/51‑1 (MQ=255)
                                                             cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                              <  1:1546709/51‑1 (MQ=255)
                                                             cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                              <  1:423090/51‑1 (MQ=255)
                                                             cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                              <  1:705027/51‑1 (MQ=255)
                                                             cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg          <  1:4010256/71‑1 (MQ=255)
                                                             cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg          <  1:3770457/71‑1 (MQ=255)
                                                             cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGgt                                        <  1:1652347/41‑1 (MQ=38)
                                                             cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGAt                   >  1:3604457/1‑62 (MQ=255)
                                                             cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg          >  1:3630361/1‑71 (MQ=255)
                                                             cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg          >  1:344535/1‑71 (MQ=255)
                                                              tGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg          >  1:316871/1‑70 (MQ=255)
                                                              tGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCtcttccg           <  1:379824/69‑3 (MQ=255)
                                                              tGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg          >  1:3299243/1‑70 (MQ=255)
                                                              tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTcc                             >  1:3721054/1‑51 (MQ=19)
                                                              tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCa                            >  1:204728/1‑52 (MQ=25)
                                                              tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCa                            >  1:4937681/1‑52 (MQ=25)
                                                              tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCa                            >  1:179118/1‑52 (MQ=25)
                                                               gTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa                            >  1:3396405/1‑51 (MQ=255)
                                                                ttCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa                            >  1:1626343/1‑50 (MQ=255)
                                                                ttCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa                            >  1:2207877/1‑50 (MQ=255)
                                                                ttCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAc       >  1:2880985/1‑71 (MQ=255)
                                                                 tcgcGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAc       >  1:2441857/4‑70 (MQ=255)
                                                                 tCCCGTTCCTGTTCATCACCATCGCCTGTGGtgcg                                          >  1:1075373/1‑35 (MQ=37)
                                                                  cccGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTc                              >  1:1554944/1‑46 (MQ=255)
                                                                  cccGTTCATGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTc                              >  1:1115606/1‑46 (MQ=255)
                                                                   ccGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCAc    <  1:5355415/71‑1 (MQ=255)
                                                                     gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGc                                 >  1:2734362/1‑40 (MQ=37)
                                                                     gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc  >  1:5588902/1‑71 (MQ=255)
                                                                     gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc  >  1:201029/1‑71 (MQ=255)
                                                                     gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc  >  1:4703036/1‑71 (MQ=255)
                                                                     |                                                                      
GCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGC  >  Exported/385702‑385841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: